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881.
Various developmental stages (early larvae to adults) of Euphausia superba have been collected in different seasons in the Weddell Sea, the Lazarev Sea and off the Antarctic Peninsula to investigate
the role of lipids and fatty acids in the life cycle of the Antarctic krill. The total-lipid data for E. superba exhibited seasonal variations, with low lipid levels in late winter/early spring and the highest levels in autumn. Seasonal
changes were most pronounced in the immature and adult specimens, increasing from about 10% lipid of dry mass to more than
40%. The fatty-acid compositions of the younger stages were dominated by 20:5(n-3), 22:6(n-3) and 16:0. These are typical
phospholipid fatty acids, which are major biomembrane constituents. The phospholipid composition was similar in the older
stages. With increasing storage of triacylglycerols in the lipid-rich immature and adult stages, the fatty acids 14:0, 16:0
and 18:1(n-9) prevailed, comprising about 70% of total triacylglycerol fatty acids. The trophic-marker fatty acids 16:1(n-7)
and 18:4(n-3), indicating phytoplankton ingestion, were less abundant. They reflected, however, the dependence of the larvae
on phytoplankton as well as the seasonal changes in algal composition. The generally close linear relationships between fatty
acids and lipid suggest that the fatty-acid compositions of the collected specimens were largely independent of the respective
developmental stage, season and region. The linear fit indicates that triacylglycerol accumulation started at a level of about
5% of total lipid. Considering the various overwintering scenarios under discussion, the life cycle and reproductive strategies
of krill are discussed in the context of the lipid metabolism and fatty-acid composition of E. superba. Lipid production is effective enough to accumulate large energy reserves for the dark season, but E. superba does not exhibit the sophisticated biosynthetic pathways known from other Antarctic euphausiids and copepods. Although important,
lipid utilisation appears to be just one of several strategies of E. superba to thrive under the extreme Antarctic conditions, and this pronounced versatility may explain the success of this species
in the Southern Ocean.
Received: 16 June 2000 / Accepted: 18 December 2000 相似文献
882.
Spatial and temporal pattern in seagrass community composition and productivity in south Florida 总被引:4,自引:1,他引:3
We document the distribution and abundance of seagrasses, as well as the intra-annual temporal patterns in the abundance
of seagrasses and the productivity of the nearshore dominant seagrass (Thalassia testudinum) in the south Florida region. At least one species of seagrass was present at 80.8% of 874 randomly chosen mapping sites,
delimiting 12,800 km2 of seagrass beds in the 17,000-km2 survey area. Halophila decipiens had the greatest range in the study area; it was found to occur over 7,500 km2. The range of T. testudinum was almost as extensive (6,400 km2), followed by Syringodium filiforme (4,400 km2), Halodule wrightii (3,000 km2) and Halophila engelmanni (50 km2 ). The seasonal maxima of standing crop was about 32% higher than the yearly mean. The productivity of T. testudinum was both temporally and spatially variable. Yearly mean areal productivity averaged 0.70 g m−2day−1, with a range of 0.05–3.29 g m−2 day−1. Specific productivity ranged between 3.2 and 34.2 mg g−1 day−1, with a mean of 18.3 mg g−1 day−1. Annual peaks in specific productivity occurred in August, and minima in February. Integrating the standing crop for the
study area gives an estimate of 1.4 × 1011 g T. testudinum and 3.6 × 1010 g S. filiforme, which translate to a yearly production of 9.4 × 1011 g T. testudinum leaves and 2.4 × 1011 g S. filiforme leaves. We assessed the efficacy of rapid visual surveys for estimating abundance of seagrasses in south Florida by comparing
these results to measures of leaf biomass for T. testudinum and S. filiforme. Our rapid visual surveys proved useful for quantifying seagrass abundance, and the data presented in this paper serve as
a benchmark against which future change in the system can be quantified.
Received: 30 January 2000 / Accepted: 24 July 2000 相似文献
883.
M. Böhm U. Hentschel A. Friedrich L. Fieseler R. Steffen V. Gamulin I. Müller W. Müller 《Marine Biology》2001,139(6):1037-1045
The aim of this study was the documentation of the molecular immune response of Suberites domuncula upon bacterial infection. Additionally, the bacteria that are naturally present in the sponge after prolonged aquarium maintenance were characterized. After 6 months of maintenance of S. domuncula in seawater aquaria, only one bacterial 16S rDNA sequence could be recovered, which belongs to the genus Pseudomonas. Concomitantly, morphologically uniform bacteria were found encapsulated in bacteriocytes. These findings indicate that certain bacteria, possibly of the genus Pseudomonas, are able to persist for long periods in host bacteriocytes. Subsequent to performing a previously established infection assay with S. domuncula, a potentially pathogenic Vibrio sp. was isolated from the tissues. Furthermore, the host tissue disintegrated and asexual propagation bodies (gemmules) were formed. In order to gain insights into the molecular events occurring after bacterial infection, the stress-response kinases, p38 protein kinase and JNK protein kinase, were analyzed. It is demonstrated that these two kinases are activated (phosphorylated) upon incubation of the tissue with the bacterial endotoxin lipopolysaccharide (LPS). Moreover, LPS strongly inhibits protein synthesis. It is concluded that there are many functionally different interactions between S. domuncula and bacteria and that the animal possesses mechanisms to differentiate between bacteria and to respond accordingly. 相似文献
884.
Effects of salinity on endogenous rhythm of the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae) 总被引:1,自引:0,他引:1
The Manila clam Ruditapes philippinarum, an intertidal bivalve, was exposed to different salinity regimes (from 31.0–31.7‰ down to 20‰, 15‰, 10‰, 5‰), and the endogenous
rhythm in its oxygen consumption was studied using an automatic intermittent-flow respirometer. When exposed to salinities
reduced from 31.5‰ to 20‰ and 15‰ under otherwise constant conditions, the clams recovered a clear endogenous circatidal rhythm
in their oxygen-consumption rate after having dampened periods of 12 h and 48 h, respectively. At salinities less than 10‰,
however, the oxygen-consumption rate was depressed greatly at the beginning of the experiment for about 36 h and then increased
to a level higher than normal, but the rhythm of oxygen consumption was not recovered. The results of this study indicate
that the Manila clam, a euryhaline organism, cannot maintain a normal metabolic activity at a salinity lower than 15‰. All
clams were dead after exposure at a salinity of 5‰ for 7 days.
Received: 28 February 2000 / Accepted: 26 August 2000 相似文献
885.
The seaweed Sargassum polyceratium Montagne inhabits a broad spectrum of subtidal and intertidal habitats. Genetic diversity and spatial genetic structure were examined within and among 12 stands using random amplified polymorphic DNA (RAPD) phenotypes. Data were analyzed using analysis of molecular variance (AMOVA) and Shannon's information measure. In both analyses, 60-75% of the variation occurred within stands and 25-40% between stands. These values are consistent with out-crossing, high-dispersal species. Significant differentiation was found among bays ca. 25 km apart (Shannon's G'st averaged 0.37 and pairwise AMOVA Kst values averaged 0.272) and among stands 150-200 m apart within bays (AMOVA Kst values averaged 0.149). Effects of shore (windward vs. leeward), depth, and bay on population structure were tested. These analyses revealed that the factor depth is confounded with shore, and that bays show significant differentiation from each other but are not completely isolated from one another. Mantel tests for differentiation-by-distance were significant along both sides of the island but stronger along the windward side. A neighbor-joining analysis of genetic distances among stands showed that the effects of currents around both tips of the island were especially important for shallow populations. For S. polyceratium, depth and bay promote population differentiation along shores, yet dispersal around the tips of the island simultaneously connects these populations to varying degrees. This study highlights the importance of investigating the relative contribution of habitat factorsin relation to island-scale population structure. 相似文献
886.
The focus of this paper is on the dynamics of a dense current flowing along the continental slope, and the frictionally induced downward motion it experiences. In particular, the movement of the lateral boundaries where the isopycnals meet the bottom are considered. The current is taken to be wide compared to the Rossby radius, which is in accordance with observations and makes the dynamics quasigeostrophic. The time development of the plume thickness is studied, using three different parameterisations for the bottom friction.Independently of the choice of parameterisation, the following results are obtained: In the central part of the plume friction acts as a diffusive process to minimise the curvature of the upper surface of the plume. At the upper edge the plume quickly approaches a state with small slope, i.e. small geostrophic velocity and small frictionally induced downward flow. At the lower edge a tongue of dense water shoots out creating a widening layer. The thickness of the migrating layer is approximately equal to the boundary layer depth and its downward speed is comparable to the along-slope geostrophic velocity. The downslope end of the migrating layer may form a steep front which requires some precautions in the numerical procedure. 相似文献
887.
Green sea urchins, Strongylocentrotus droebachiensis OF Müller, collected off the coast of New Hampshire, USA, in late February 1995, were brought into the laboratory and fed
an artificial diet ad libitum, and subjected to a photoperiod advanced by 4 months. During this study, temperatures and salinities
for experimental urchins mirrored those recorded at the collection site. We examined the effects of changes in feeding regime
and photoperiod on gametogenesis and compared the experimental urchins with those from the source population. During the 7-month
period, experimental urchins showed no detectable changes in mean test height or diameter. Experimental urchins had a significantly
higher gonad index (GI) in March, April and May (18 ± 6%) compared with field urchins in March (11 ± 3%). Subsequently, experimental
urchins had a mean monthly GI of 25 to 30%, while the mean GI for field urchins was 11 to 13%. Gonial cell mitosis and gametogenesis
occurred earlier in experimental male and female urchins compared with field urchins. Stereological and histological observations
and stage–frequency data showed that the ovaries of experimental urchins were large because of the accelerated development
of nutritive phagocytes, the volume fraction (V
v) of which was 89 to 90% of the gonad, while new vitellogenic primary oocytes occupied <1% V
v. In males, stereological and histological observations and stage–frequency data suggested a mobilization of materials from
the nutritive phagocytes beginning between June and August, i.e. earlier than in females, and, by September, new gametes occupied
a V
v of 49 ± 3% of the testes. Oocyte size–frequency distributions demonstrated that most primary oocytes were <80 μm in diameter
between March and September, suggesting that cold temperatures may be needed for completion of vitellogenesis. We describe
changes in the two principal cell types in the germinal epithelium of urchin gonads and indicate how knowledge of their population
dynamics may be useful in aquaculture applications.
Received: 21 February 1997 / Accepted: 3 June 1998 相似文献
888.
The predation impact of the two chaetognaths Eukrohnia hamata and Sagitta gazellae on mesozooplankton standing stock were investigated in three depth layers during two 24 h stations occupied in the vicinity
of Marion Island in late austral summer (April/May) 1986. The zooplankton community at both stations was dominated by small
copepods (Oithona spp., Microcalanus spp.), which accounted for >95% of total zooplankton abundance. Chaetognaths comprised <2% of total zooplankton abundance.
E. hamata constituted >95% of the total chaetognath stock. The general trend in both species was decreasing abundance with increasing
depth, which appeared to be correlated to the distribution of copepods (r
2 = 0.45; P <0.05). Gut-content analysis showed that copepods (mainly Oithona spp., Calanus spp. and Rhincalanus gigas) and ostracods were the main prey of both species, accounting for 87 and 61% of the total number of prey in E. hamata and S.␣gazellae stomachs, respectively. In the guts of S.␣gazellae, pteropods (Limacina spp.) and chaetognaths were also well represented. The mean number of prey items (NPC) for E. hamata ranged from 0.02 to 0.06 prey individual−1 which corresponds to an individual feeding rate (Fr) of between 0.05 and 0.12 prey d−1. For S.␣gazellae, the NPC values were higher, varying between 0.04␣and 0.20 prey individual−1, or between 0.15 and 0.76 prey d−1. The daily predation impact of the two chaetognaths was estimated at between 0.3 and 1.2% of the copepod standing stock or
between 7 and 16% of the daily copepod production. Predation by S. gazellae on chaetognaths accounted for up to 1.6% of the chaetognath standing stock per day.
Received: 26 November 1996 / Accepted: 31 October 1997 相似文献
889.
Common shrimp, Crangon crangon (L.), were exposed to inorganic arsenic (arsenate), trimethylarsine oxide, or arsenobetaine in sea water (100 μg As l−1) or in food (1 mg As g−1 wet wt) for up to 24 d, followed by 16 d depuration in clean sea water with undosed food, in order to determine the efficiency
of uptake and retention of the compounds. Accumulation of arsenic in the tail muscle, gills, midgut gland, exoskeleton, and
remaining tissues was found to depend on the chemical form of the arsenic and the route of exposure. No arsenic was accumulated
by C. crangon exposed to arsenate or trimethylarsine oxide in sea water. Shrimps exposed to waterborne arsenobetaine initially accumulated
a small amount of arsenic in their tail muscle and gills. After 16 d, C. crangon fed arsenate, trimethylarsine oxide, or arsenobetaine had accumulated arsenic in their tail muscle to levels ∼2-, 2-, or
40-times, respectively, that of the control group. A roughly linear rate of accumulation was shown by shrimps fed trimethylarsine
oxide or arsenobetaine, but C. crangon fed arsenate accumulated arsenic for 16 d, then lost arsenic such that their concentration on Day 24 was not significantly
different from that of the control group. Patterns of arsenic accumulation in the gills of shrimps fed the compounds were
similar to those seen in the tail muscle. On a whole animal basis, C. crangon retained ∼1.2% of the arsenate, 1.6% of the trimethylarsine oxide, and 42% of the arsenobetaine consumed over the first 16 d
of exposure, with roughly half present in the tail muscle in each case. Data obtained support the view that the direct uptake
of arsenobetaine from sea water does not make a significant contribution to the relatively high concentrations of this compound
in marine crustaceans, and that food is the primary source. Naturally occurring arsenic compounds in C. crangon and possible transformations of the administered arsenic compounds were examined by high performance liquid chromatography
using an inductively coupled plasma mass spectrometer as the arsenic-specific detector. Control C. crangon contained arsenobetaine as the major arsenic compound (>95% of total arsenic); tetramethylarsonium ion (0.7%) and an unknown
arsenic compound (1.7%) were also present as minor constituents. Shrimp ingesting arsenobetaine accumulated it unchanged.
Shrimp ingesting arsenate did not form methylated arsenic compounds; they appeared to contain their accumulated arsenic as
unchanged arsenate only, although the possibility that some of the arsenic was reduced to arsenite could not be excluded.
C. crangon ingesting trimethylarsine oxide biotransformed the compound predominantly to dimethylarsinate.
Received: 9 October 1997 / Accepted: 11 February 1998 相似文献
890.
Larval development in Amphiglena mediterranea is direct in the parental tube, and larvae crawl away as young juveniles with a radiolar crown capable of feeding. The non-feeding
larvae of A. nathae are initially brooded in the parental tube, and then have a swimming phase before settling and developing a radiolar crown.
Developmental data and reproductive features such as sperm structure are combined with other morphological data for an assessment
of the cladistic relationships of the species in the genus Amphiglena. Two species of Laonome are included as part of the ingroup. Six genera, representing a sister group to the Laonome/Amphiglena clade, are used as an outgroup. Two most-parsimonious cladograms were found, and the evolution of reproductive features are
discussed. The monophyly of Amphiglena is indicated by features previously identified, as well as two features based on sperm morphology. At present a plesiomorphic
larval form cannot be identified for the genus Amphiglena.
Received: 16 June 1997 / Accepted: 29 January 1998 相似文献