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41.
Solvent extraction and evaporative concentration steps are often used in procedures for the measurement of butyltins in environmental samples. As part of a larger study utilizing radiolabeled butyltins, the loss and fractionation of butyltins during sample preparation was investigated. TBT, DBT, and MBT were extracted from acidified seawater by hexane with efficiencies of about 95–99, 50–60 and 11% respectively. In addition, losses of about 70% of DBT were found during evaporative concentration of hexane. A variety of sediment extraction procedures were tested and none were found to be highly efficient for total butyltin extraction. 相似文献
42.
Protein that makes sense in the Argentine ant 总被引:1,自引:0,他引:1
With a protein-based approach, we have identified and cloned the cDNA encoding a chemosensory protein (LhumCSP) in the Argentine ant, Linepithema humile. The open reading frame of the cloned cDNA encoded a signal peptide (20 residues), and a mature protein (pI 4.62) of 106 amino acid residues. The calculated molecular mass (12,453 Da) was in agreement with the molecular mass measured by on-line chromatography-electrospray ionization mass spectrometry (12,448 Da), given the formation of two disulfide bridges. LhumCSP shared sequence similarity with various CSPs, particularly those identified and/or cloned from moth species. Also, LhumCSP showed the hallmark of the chemosensory proteins, i.e., four well conserved cysteine residues. The antennal protein was not detected in non-olfactory tissues (leg and thorax) contrary to a putative pheromone-binding protein isolated from the thorax of the red imported fire ant, Solenopsis invicta. In addition, these findings suggest that, as in Orthopterans and Phasmids, the protein that makes sense in the Argentine ant is not an odorant-binding protein, but rather a chemosensory protein. 相似文献
43.
Nectar acquisition in the honeybee Apis mellifera is a partitioned task in which foragers gather nectar and bring it to the hive, where nest mates unload via trophallaxis
(i.e. mouth-to-mouth transfer) the collected food for further storage. Because forager mates exploit different feeding places
simultaneously, this study addresses the question of whether nectar unloading interactions between foragers and hive-bees
are established randomly, as it is commonly assumed. Two groups of foragers were trained to exploit a different scented food
source for 5 days. We recorded their trophallaxes with hive-mates, marking the latter ones according to the forager group
they were unloading. We found non-random probabilities for the occurrence of trophallaxes between experimental foragers and
hive-bees, instead, we found that trophallactic interactions were more likely to involve groups of individuals which had formerly
interacted orally. We propose that olfactory cues present in the transferred nectar promoted the observed bias, and we discuss
this bias in the context of the organization of nectar acquisition: a partitioned task carried out in a decentralized insect
society. 相似文献
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48.
A Simulation of Temporal and Spatial Variations in Carbon at Landscape Level: A Case Study for Lake Abitibi Model Forest in Ontario,Canada 总被引:2,自引:0,他引:2
Xiaolu Zhou Changhui Peng Qing-Lai Dang Jiaxin Chen Sue Parton 《Mitigation and Adaptation Strategies for Global Change》2007,12(4):525-543
Using a case study of the Lake Abitibi Model Forest (LAMF), this study aims to assess the temporal and spatial variability
in carbon storage during 1990–2000, and to present a comprehensive estimation of the carbon budget for LAMF's ecosystems.
As well, it provided the information needed by local forest managers to develop ecological and carbon-based indicators and
monitor the sustainability of forest ecosystems. Temporal and spatial carbon dynamics were simulated at the landscape level
using ecosystem model TRIPLEX1.0 and Geographical Information System (GIS). The simulated net primary productivity (NPP) and
carbon storage in forest biomass and soil were compared with field data and results from other studies for Canada's boreal
forests. The results show that simulated NPP ranged from 3.26 to 3.34 tC ha−1 yr−1 in the 1990s and was consistent with the range measured during the Boreal Ecosystem-Atmosphere Studies (BOREAS) in central
Canada. Modeled NPP was also compared with the estimation from remote sensing data. The density of total above-and belowground
biomass was 125.3, 111.8, and 106.5 tC ha−1 for black spruce, trembling aspen, and jack pine in the LAMF ecosystem, respectively. The total carbon density of forested
land was estimated at 154.4 tC ha−1 with the proportion of 4:6 for total biomass and soil. The analysis of net carbon balance of ecosystem suggested that the
LAMF forest ecosystem was acting as a carbon sink with an allowable harvest in the 1990s. 相似文献
49.
In angiosperms, archesporial cells in the anther primordium undergo meiosis to form haploid pollen, the sole occupants of anther sacs. Anther sacs are held together by a matrix of parenchyma cells, the connective tissue. Cells of the connective tissue are not known to differentiate. We report the differentiation of parenchyma cells in the connective tissue of two Gordonia species into pollen-like structures (described as pseudopollen), which migrate into the anther sacs before dehiscence. Pollen and pseudopollen were distinguishable by morphology and staining. Pollen were tricolpate to spherical while pseudopollen were less rigid and transparent with a ribbed surface. Both types were different in size, shape, staining and surface architecture. The ratio of the number of pseudopollen to pollen was 1:3. During ontogeny in the connective tissue, neither cell division nor tetrad formation was observed and hence pseudopollen were presumed to be diploid. Only normal pollen germinated on a germination medium. Fixed preparations in time seemed to indicate that pseudopollen migrate from the connective tissue into the anther sac. 相似文献
50.