Coagulation is the best available method for removing intracellular organic matter (IOM), which is released from algae cells and is an important precursor to disinfection by-products in drinking water treatment. To gain insight into the best strategy to optimize IOM removal, the coagulation performance of two Al salts, i.e., aluminum chloride (AlCl3) and polyaluminum chloride (PACl, containing 81.2% Al13), was investigated to illuminate the effect of Al species distribution on IOM removal. PACl showed better removal efficiency than AlCl3 with regard to the removal of turbidity and dissolved organic carbon (DOC), owing to the higher charge neutralization effect and greater stability of pre-formed Al13 species. High pressure size exclusion chromatography analysis indicated that the superiority of PACl in DOC removal could be ascribed to the higher binding affinity between Al13 polymer and the low and medium molecular weight (MW) fractions of IOM. The results of differential log-transformed absorbance at 254 and 350?nm indicated more significant formation of complexes between AlCl3 and IOM, which benefits the removal of tryptophan-like proteins thereafter. Additionally, PACl showed more significant superiority compared to AlCl3 in the removal of < 5?kDa and hydrophilic fractions, which are widely viewed as the most difficult to remove by coagulation. This study provides insight into the interactions between Al species and IOM, and advances the optimization of coagulation for the removal of IOM in eutrophic water. 相似文献
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 103 to 7.5 × 103 genome copies per liter (GC l−1), using the N1 RT-qPCR assay, and from 2.4 × 102 to 5.9 × 103 GC l−1 using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area.
The Soil and Water Assessment Tool (SWAT) model (Arnold et al., 1998) is a popular watershed management tool. Currently, the SWAT model, actively supported by the U.S. Department of Agriculture and Texas A&M, operates only on Microsoft® Windows, which hinders modelers that use other operating systems (OS). This technical note introduces the Comprehensive R Archive Network (CRAN) distributed “SWATmodel” package which allows SWAT 2005 and 2012 to be widely distributed and run as a linear model‐like function on multiple OS and processor platforms. This allows researchers anywhere in the world using virtually any OS to run SWAT. In addition to simplifying the use of SWAT across computational platforms, the SWATmodel package allows SWAT modelers to utilize the analytical capabilities, statistical libraries, modeling tools, and programming flexibility inherent to R. The software allows watershed modelers to develop a simple hydrological watershed model conceptualization of the SWAT model and to obtain a first approximation of the minimum expected results a more complicated model should deliver. As a proof of concept, we test the SWAT model by initializing and calibrating 314 U.S. Geological Survey stream gages in the Chesapeake Bay watershed and present the results. 相似文献
Sustainability science is at an early stage of development. Among many other obstacles, there are two prominent issues hindering its advance. There is both a lack of a set of principles for knowledge construction, and a need to implement research to solve real problems. This paper proposes a typology of scientific reflections for meeting these two challenges and contributing to sustainability science development. This typology is made up of four kinds of reflection: practical, instrumental-methodological, theoretical-conceptual, and onto-epistemological. Each kind of reflection is based on a different type of question and gives shape to its respective type of research. 相似文献
Increased reliance on the Internet for critical infrastructure and the global nature of supply chains provides an opportunity for adversaries to leverage dependencies and gain access to vital infrastructure. Traditional approaches to assessing risk in the cyber domain, including estimation of impacts, fall short due to uncertainty in how interconnected systems react to cyber attack. This paper describes a method to represent the pathways of disruption propagation, evaluate the macroeconomic impact of cyber threats and aid in selecting among various cybersecurity policies. Based on state of the art agent-based modeling, multicriteria decision analysis, and macroeconomic modeling tools, this framework provides dynamic macroeconomic, demographic and fiscal insights regarding shocks caused by cyber attacks to the regional economy over time. The interlinkage of these models will provide a robust and adaptive system that allows policy makers to evaluate complex issues such as cybersecurity threats and their impacts on the geopolitical, social, environmental, and macroeconomic landscape. 相似文献