Using Public Participation Geographic Information Systems to Identify Places of Watershed Service Provisioning

In this study, we used public participation geographic information systems methods to collect spatial data identifying places that stakeholders in Mobile Bay, Alabama think are important providers of watershed services. These methods allowed us to spatially analyze participatory data from general public respondents and directly compare them with other scientific data in a geographic information systems database. This study identified which places in the region participants believe are important providers of specific watershed services, including fish nurseries, storm protection, flood protection, and water quality protection, which would likely have public support for conservation. Additionally, we assessed the accuracy of participant watershed service identification using land cover data to identify inconsistencies and participant knowledge gaps. This information can be used to target outreach education efforts. We found that the accuracy with which participants correctly identified places with the necessary land cover to provide each service varied considerably. We believe this to be a useful tool for managers to elicit stakeholder input and to identify knowledge gaps regarding the provisioning of watershed services.     

Influx,transport, and accumulation of cadmium in plant species grown at different Cd2+ activities

Abstract

Cadmium (Cd) has no known essential biological function, but it is toxic to plants, animals, and humans. A promising approach to prevent Cd from entering the food chain would be to select and/or create Cd‐accumulating plants to remediate contaminated soils or to develop Cd‐excluding plants to reduce Cd flow from soils into foods. The present study was undertaken to examine the differences in Cd influx, transport, and accumulation among five plant species in relation to plant tolerance to Cd toxicity. Ryegrass (Lolium perenne L.) had the least reduction in dry matter which may be due to its lowest Cd transport rate (TR) to shoots at all Cd levels among the plant species tested. White‐clover (Trifolium repens L.) was the most sensitive species to Cd toxicity, likely because of its highest Cd influx rate (IR) and high TR when plants were grown at low Cd²⁺ activity (≤8 μM). The high tolerance of cabbage (Brassica oleracea var. capitata L.) to moderate Cd toxicity (≤14 μM) appeared to be mainly due to the detoxification of Cd inside plant tissue since it recorded the highest TR and relatively high IR for Cd among the tested species. At Cd²⁺ activities up to 28 uM, the Cd uptake ratios of shoot/root for ryegrass were, on average, about 50‐fold and 27‐fold lower than that for cabbage and maize (Zea mays L.), respectively. These results showed that Cd could be easily transported into shoots of cabbage and maize, but was mainly confined to roots of ryegrass. We suggest that influx and transport rates, especially transport rate, could be used as plant physiological parameters for screening Cd‐excluding genotypes among monocotyledonous plants.     

Linking abundance and diversity of sponge-associated microbial communities to metabolic differences in host sponges

Many sponge species contain large and diverse communities of microorganisms. Some of these microbes are suggested to be in a mutualistic interaction with their host sponges, but there is little evidence to support these hypotheses. Stable nitrogen isotope ratios of sponges in the Key Largo, Florida (USA) area grouped sponges into species with relatively low δ¹⁵N ratios and species with relatively high δ¹⁵N ratios. Using samples collected in June 2002 from Three Sisters Reef and Conch Reef in the Key Largo, Florida area, transmission electron microscopy (TEM) and denaturing gradient gel electrophoresis were performed on tissues of the sponges Ircinia felix and Aplysina cauliformis, which are in the low δ¹⁵N group, and on tissue of the sponge Niphates erecta, which is in the high δ¹⁵N group. Results showed that I. felix and A. cauliformis have large and diverse microbial communities, while N. erecta has a low biomass of one bacterial strain. As the low δ¹⁵N ratios indicated a microbial input of nitrogen, these results suggested that I. felix and A. cauliformis were receiving nitrogen from their associated microbial community, while N. erecta was obtaining nitrogen solely from external sources. Sequence analysis of the microbial communities showed a diversity of metabolic capabilities among the microbes of the low δ¹⁵N group, which are lacking in the high δ¹⁵N group, further supporting metabolic differences between the two groups. This research provides support for hypotheses of mutualisms between sponges and their associated microbial communities.