Bewertung heterogener Altlasten durch Probenahme, statistische und geostatistische Modellierung

A slack heap in Saxony serves as an example for the model procedure of evaluation heterogeneous waste sites via sampling as well as statistical and geostatistical data analysis. This paper demonstrates the usefulness of the known regulations for sampling and of the tried and proven geostatistical methods developed for assessing homogeneous wastes and the concornitantly contaminated soil. Emphasized here are the contents of iron and sulfur. Considered in addition are the concentrations of arsenic and the heat loss. To establish a conclusion, the variograms of the original data are used to evaluate the waste site in different directions and at determined depths. With the aid of a spot interpolation, further data can be calculated for additional sites which have not been sampled and can be used consequently for displaying the spatial variability. Finally, the statistical data analysis is applied using the standard methods of random sampling as an alternative to the geostatistical modeling. In this manner, the consequences for the sampling strategy can be demonstrated.     

Do changes in risk-taking affect habitat shifts of sticklebacks?

The distribution of individuals is often the outcome of conflicting demands, such as between predator avoidance and reproduction. A factor that has seldom been considered in studies on habitat choice is time-dependent changes in risk-taking. We investigated the distribution of threespine sticklebacks, Gasterosteus aculeatus, over two breeding seasons and found it to change with time towards shallower areas with a more open habitat structure. Shallow and structurally less complex habitats were probably favorable due to a higher reproductive rate, but costly due to an increased risk of predation. Contrary to expectation, changing predation pressure was not a predictor of the shift in habitat use and, thus, not the proximate cue. Instead date was the main predictor. This suggests that increased risk-taking in relation to predation contributed to the habitat shift. The possibility was supported by a laboratory experiment that showed sticklebacks to take larger risks and prefer more predator-exposed areas at the end of the season than at the start of the season. These results demonstrate that temporal changes in risk-taking occur and can influence habitat choice, which points to the importance of considering risk-taking, in addition to predation pressure, when studying the effect of predators on distribution.Communicated by J. Krause     

Rapid karyotyping in prenatal diagnosis: A comparative study of the ‘pipette method’ and the ‘in situ’ technique for chromosome harvesting

Rapid karyotyping in the second and third trimesters has important implications for the management of pregnancies at risk. From September 1985 to March 1992, 735 amniotic fluid samples sent to our laboratory for rapid karyotyping from 64 different diagnostic centres of the Federal Republic of Germany were included in a comparative study on harvesting for chromosome analysis using the ‘pipette method’ or the ‘in situ’ technique. The average time between preparation of the amniotic fluid and verbal notification of the analysed karyotype was 5·41 days. The ‘pipette method’ needed on average 4·65 days, and the ‘in situ’ technique 5·97 days. In comparison with other more invasive techniques available for rapid karyotyping such as cordocentesis and placental biopsy, amniocentesis and subsequent chromosome harvesting using the ‘pipette method’ and/or the ‘in situ’ technique proved very useful and efficient. The overall incidence of chromosome aberrations was 15·3 per cent. The high rate of structural chromosome aberrations and uncommon aneuploidies found in our investigation (12 per cent) indicates that for rapid karyotyping in the second and third trimesters, conventional cytogenetic techniques cannot be replaced by faster techniques based on fluorescent in situ hybridization on interphase cells in the near future.     

The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana

We investigated the role of glutathione(GSH) and phytochelatins(PCs) on the detoxification of selenite using Arabidopsis thaliana. The wild-type(WT) of Arabidopsis thaliana and its mutants(glutathione deficient Cad 2–1 and phytochelatins deficient Cad 1–3) were separately exposed to varying concentrations of selenite and arsenate and jointly to both toxicants to determine their sensitivities. The results of the study revealed that, the mutants were about 20-fold more sensitive to arsenate than the WT, an indication that the GSH and PCs affect arsenate detoxification. On the contrary, the WT and both mutants showed a similar level of sensitivity to selenite, an indication that the GSH and PCs do not significantly affect selenite detoxification. However, the WT is about 8 times more sensitive to selenite than to arsenate, and the mutants were more resistant to selenite than arsenate by a factor of 2. This could not be explained by the accumulation of both elements in roots and shoots in exposure experiments. The co-exposure of the WT indicates a synergistic effect with regards to toxicity since selenite did not induce PCs but arsenic and selenium compete in their PC binding as revealed by speciation analysis of the root extracts using HPLC–ICP–MS/ESI–MS. In the absence of PCs an antagonistic effect has been detected which might suggest indirectly that the formation of Se glutathione complex prevent the formation of detrimental selenopeptides. This study, therefore, revealed that PC and GSH have only a subordinate role in the detoxification of selenite.