The effect of cadmium on stage II naupliar larvae of barnacle (Balanus amphitrite Darwin) was investigated. Barnacle larvae were exposed to 0-15 microM CdCl(2) for 24 h. Apoptotic cells were stained by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling (TUNEL) method. Incidence of apoptosis, as measured by numbers of animals with ectopic pattern of apoptosis as well as numbers of apoptotic cells per animal, was assessed using confocal microscopy. An increase in incidence of apoptosis was observed in the experimental animals with an increase in cadmium concentration. Mortality increased, and motility decreased, when barnacle larvae were exposed to an increasing concentration of cadmium. The relationship between the occurrence of apoptosis and swimming behaviour was investigated in larvae exposed to 10 microM CdCl(2). Significant increases in apoptosis were detected in the non-motile and dead nauplii. This study suggested that whole-mount in situ TUNEL method may be used to study increased occurrence of apoptotic cells in crustacean larvae. 相似文献
Informal trails created or perpetuated by visitors is a management challenge in many protected natural areas such as Yosemite
National Park. This is a significant issue as informal trail networks penetrate and proliferate into protected landscapes
and habitats, threatening ecological integrity, aesthetics, and visitor experiences. In order to develop effective strategies
for addressing this problem under an adaptive management framework, indicators must be developed and monitoring protocol must
be established to gather timely and relevant data about the condition, extent, and distribution of these undesired trail segments.
This article illustrates a process of developing and evaluating informal trail indicators for meadows in Yosemite Valley.
Indicator measures developed in past research were reviewed to identify their appropriateness for the current application.
Information gaps in existing indicator measures were addressed by creating two new indices to quantify the degree of informal
trailing based on its land fragmentation effects. The selected indicator measures were applied to monitoring data collected
between 2006 and 2008. The selected measures and indices were evaluated for their ability to characterize informal trail impacts
at site and landscape scales. Results demonstrate the utility of indicator measures in capturing different characteristics
of the informal trail problem, though several metrics are strongly related to each other. The two fragmentation indices were
able to depict fragmentation without being too sensitive to changes in one constituent parameter. This study points to the
need for a multiparameter approach to informal trail monitoring and integration with other monitoring data. Implications for
monitoring programs and research are discussed. 相似文献
This study was carried out to evaluate longevity of available organic materials used for sulfate-reducing bacteria (SRB) activity in vertical flow ponds (VFPs) to treat mine drainage in South Korea. Spent mushroom compost samples (SMC) were tested as substrates in VFPs and analyzed for total organic carbon in VFPs, and were collected to analyze total organic carbon (TOC), T-N, T-P, K, metals and residual cellulose to check the longevity assessment. Chemical analysis revealed that the average contents of Fe, Al and Mn in SMC of VFPs were 19,907, 32,137 and 434 mg/kg, respectively. The contents of Fe and Al in SMC of VFPs were much higher than those of the unused SMC (control), but to the contrary, those of Mn showed a reversed tendency. Average TOC content of the controls was 64.19% but in one of the VFP substrates was as low as 15.92%. This might be resulted from SRB consumed the available organic carbon in SMC as VFPs system aged. Contents of T-N in VFPs tended to decrease as VFPs aged. The residual cellulose ranged from 3.88 to 6.72% (g/g). There existed a negative relationship between residual cellulose contents and ages of VFPs. Assuming that SMC in all VFPs had similar compositions when the VFPs were initially established, trend analysis predicted that the amount of carbon source for SRB might be available for 12–15 years further, depending on VFPs.