In this research, supercritical carbon dioxide extraction (SFE) showed better extraction effect when compared with Solid- liquid extraction (SLE), Soxhlet extraction (SE) and Ultrasonic extraction (UE), not only in the rate but also the time. The comparison among these three extraction modifiers, including acetone, ethanol and methanol demonstrated that ethanol was preferred to SFE due to its high extraction effect and low toxicology. In addition, parameter of SFE, influence of temperature and pressure were investigated, and the best extraction effect was achieved at the optima conditions, temperature of 40°C and the pressure of 35 MPa. Thus, SFE is a highly effective method for flavonols extraction, requiring minimum energy and producing non-toxic byproduct. SFE-GC system is applied for the evaluation on flavonols that plays a key role in plant resistance to heavy metal, with its content and synthetase gene expression significantly increasing in plant when threatened by heavy metal. Besides, results indicated that flavonols can improve plant resistance to oxidative stress by quenching the redundant ROS in matrix.
In this study, the current situation of five types of toxic organics and endocrine disrupters in the sediments of rivers around Beijing, i.e., polycyclic aromatic hydrocarbons (PAHs), phthalic acid esters (PAEs), organic chlorinated pesticides (OCPs), estrogens (Es), and bisphenol A (BPA), which included 56 contaminants, was analyzed and compared with that registered by the historical literatures. The ecological risks were also assessed. The total concentration of PAHs, PAEs, OCPs, Es, and BPA ranged from 232.5 ng·g–1 to 5429.7 ng·g–1, 2047.2 ng·g–1 to 18051.5 ng·g–1, 4.5 ng·g–1 to 11.7 ng·g–1, 18.1 ng·g–1 to 105.2 ng·g–1, and 36.3 ng·g–1 to 69.6 ng·g–1, respectively. Among these five types of organic compounds, the concentration levels of PAHs and OCPs have decreased significantly in the last ten years, while those of PAEs and Es had an upward trend compared with the previous studies. BPA still remained at a moderately high level, as it was ten years ago. The risks of the PAEs in all of the sample sites, and fluoranthene, benzo[a]anthrene, and benzo[a]pyrene in the Wenyu River sediment, were relatively high. These results supplemented the database of toxic organics’ concentration levels in the sediments of Beijing rivers.
While multi-stakeholder collaboration is critical for effective community post-disaster reconstruction (CPDR), it is often very difficult in practice. The Longmen Shan Fault active seismic zone in China has experienced several recent earthquakes with the 2008 Wenchuan earthquake and 2013 Lushan earthquake, both of which caused extensive and widespread damage to many communities, presenting unprecedented challenges for post-disaster reconstruction. This paper develops a multi-cycle field research method that involves three interconnected cycles: internet research, field visits, and extensive surveys: to examine multi-stakeholder collaboration for the CPDR following the Wenchuan earthquake. It was found that there were 12 types of active stakeholders engaged across four main areas: infrastructure rebuilding, psychological recovery, socio-economic rehabilitation, and ecological restoration. Specifically, local community participation and effective collaboration between the community and the external stakeholders were found to be the most crucial elements for successful reconstruction. Multi-stage field research after the Lushan earthquake verified that CPDR was effective and that multi-stakeholder collaboration had improved from the lessons learnt from the Wenchuan earthquake reconstruction experience. Some advantages and limitations of this research are also given. 相似文献
