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671.
四环素对大肠杆菌抗生素抗性基因进化的影响 总被引:1,自引:0,他引:1
为了解环境中低浓度抗生素对抗生素抗性发展的影响,以大肠杆菌(Escherichia coli,E.coli)为基础材料,通过亚抑菌浓度四环素处理,得到由敏感到抗性的菌株,研究了四环素对大肠杆菌产生抗生素抗性基因(antibiotic resistance genes,ARGs)的影响,采用同位素标记相对和绝对定量技术(isobaric tags for relative and absolute quantification,iTRAQ),分析四环素处理前后,大肠杆菌总蛋白差异表达情况.结果表明,大肠杆菌经1/2MIC浓度的四环素诱导,20 d后可由敏感转至耐药,表明环境中的低浓度四环素的刺激对大肠杆菌耐药性的形成影响较大.并且,检测到四环素抗性基因tetA、tetE和tetZ,β-内酰胺类抗性基因blaTEM和blaFOX,多重耐药性基因acrA、marA和marR.蛋白结果表明大肠杆菌在四环素胁迫下以特定的节能存活模式,通过诱导细胞防御和修复系统来应对环境压力,并表现出对离子传输的严格调节,来抵抗四环素毒性的影响.研究结果为进一步解析环境中抗生素胁迫的抗性机制提供了参考. 相似文献
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Guocheng Hu Xiaojun Luo Fengchao Li Jiayin Dai Jianyang Guo Shejun Chen Cao Hong Bixian Mai Muqi Xu 《环境科学学报(英文版)》2010,22(2):176-183
Organochlorine pesticides(OCPs),polychlorinated biphenyls(PCBs),and polycyclic aromatic hydrocarbons(PAHs) were determined in nineteen surface sediment samples collected from Baiyangdian Lake and its inflowing river(Fuhe River) in North China.Total concentrations of OCPs,PCBs and PAHs in sediments ranged from 5.4 to 707.6 ng/g,2.3 to 197.8 ng/g,and 101.3 to 6360.5 ng/g,respectively.The levels of contaminants in Fuhe River were significantly higher than those in Baiyandian Lake.For hexachlorocyclohexane(HCHs) and dichlorodiphenytrichloroethanes(DDTs),α-HCH and p,p-DDT were predominant isomers;while for PCBs,PCB 28/31,PCB 40/103,PCB 60,PCB 101,and PCB 118 were predominant congeners.Possible sources derived from historical usage for OCPs and incomplete combustion fuel,wood,and coal and exhaustion of boats or cars for PAHs.Risk assessment of sediment indicated that sediments in Fuhe River were likely to pose potential biological adverse impact. 相似文献
673.
采用垂直流人工湿地模拟装置对新疆油田外排含盐稠油废水进行了处理。实验表明:对于进水CODCr为402~406mg/L,盐度5701~5712mg/L,石油类40.62mg/L的含盐稠油废水,该系统的出水指标为CODCr35~38mg/L,盐度1535~1542mg/L,湿地系统对CODCr和盐分的去除率达到91%和73%;当水力停留时间为11d以上,出水石油类<5mg/L,处理后出水CODCr、石油类达到《污水综合排放标准》(GB8978-1996)的一级标准。 相似文献
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Fang Fang Yan Yang Jinsong Guo Hong Zhou Chuan Fu Zhe Li 《Frontiers of Environmental Science & Engineering in China》2011,5(3):426-434
Three-dimensional fluorescence spectroscopy was used to investigate the fluorescent properties of soil dissolved organic matter
(DOM) in the water-level-fluctuation zone (WLFZ) of Kai County, Three Gorges Reservoir (TGR). Most of the soil DOM analyzed
in this study was found to contain four fluorescence peaks. Peaks A and C represent humic-like fluorescence, whereas peaks
B and D represent tryptophan-like fluorescence. Peaks E and F, which represent tyrosine-like fluorescence, only appeared in
certain soils. Soil humus was the main source of DOM in soil, and higher concentration of soil DOM was found in the exposed
soil than submerged soil. Compared to the peaks A and B, the fluorescence intensities of peaks C and D were strongly influenced
by the fluctuating water level. Analysis of fluorescence intensities of different peaks in soil DOM showed that WLFZ soil
was not contaminated significantly. Soil DOM contained at least two types of humic-like fluorescence groups and two types
of protein-like fluorescence groups. The proportion of the content of peak A in soil organic matter was quite stable. The
soil DOM in exposed soil had relatively high humification and aromaticity, and periodic submerging and exposure of soil had
an impact on the humification of soil DOM. 相似文献
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Arsenic(As) and antimony(Sb) are usually coexistent in mine wastes and pose a great threat to human health. The As immobilization by nano zero-valent iron(n ZVI) is promising, however, the stabilization for co-occurring As and Sb is not known. Herein, the immobilization and transformation of As and Sb in n ZVI-treated sediments were evaluated using complementary leaching experiments and characterization techniques. Raw sediment samples from a gold-antimony deposit revealed the co-existence of ul... 相似文献