A new method for specific antibody production was developed using antibody (Ab)-pesticide complex as a unique immunogen. Parathion (PA) was the targeted pesticide, and rabbit polyclonal antibody (Pab) and mouse monoclonal antibody (Mab) were used as carrier proteins. The Ab-PA complexes were generated by conjugating the two antibodies with an excessive dosage of PA. It was shown that the sensitivity of homologous enzyme-linked immunosorbent assay (ELISA) using the new antibodies was similar to that using original antibodies. However, the new mouse Pab had not only similar positive recognizing spectrum as the original Mab, but also a significantly improved sensitivity in heterologous ELISA when some recognizable competitors were applied. IC50 value of ELISA based on a combination of the new mouse Pab and hapten 9 was 0.24 ng/mL, which was 445.54 times as that of the homologous ELISA. An Ab-pesticide complex may be a suitable alternative immunogen for producing highly specific antibody to improve the immunoassay sensitivity. 相似文献
Human activities have increased anthropogenic CO2 emissions, which are believed to play important roles in global warming. The spatiotemporal variations of CO2 concentration and flux at fine spatial scales in aquaculture ponds remain unclear, particularly in China, the country with the largest aquaculture. In this study, the plot-scale spatiotemporal variations of water CO2 concentration and flux, both within and among ponds, were researched in shrimp ponds in Shanyutan Wetland, Min River Estuary, Southeast China. The average water CO2 concentration and flux across the water–air interface in the shrimp ponds over the shrimp farming period varied from 22.79?±?0.54 to 186.66?±?8.71 μmol L?1 and from ??0.50?±?0.04 to 2.87?±?0.78 mol m?2 day?1, respectively. There was no remarkable difference in CO2 concentration and flux within the ponds, but significantly spatiotemporal differences in CO2 flux were observed between shrimp ponds. Chlorophyll a, pH, salinity, air temperature, and morphometry were the important factors driving the spatiotemporal patterns of CO2 flux in the shrimp ponds. Our findings highlighted the importance and spatiotemporal variations of CO2 flux in the important coastal ecosystems.
Hexahedron-like BiPO4 microcrystals were sucessfully synthesized via a template-free hydrothermal method. The resulting samples were characterized by Xray diffraction (XRD), field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and UV-vis spectroscopy. The BiPO4 samples were of pure monoclinic phase, and the initial amount of PO3–4 during synthesis did not show obvious effect on the phase properties of the materials. The hexahedron-like BiPO4 microcrystal had explicitly cut edges, and its thickness was about 1 μm. The photocatalytic performance of the BiPO4 catalysts was evaluated by photodegradation of RhB under UV light irradiation with commerial Degussa P25 TiO2 as reference. Compared with P25, the BiPO4 catalysts displayed higher photocatalytic activity, with 98.7% of RhB degraded during 60-min experiment. Cost evaluation analysis was adopted to describe the energy consumption of the degradation process, and the results suggested the potential application of this material in the field of dye-contaminated wastewater treatment or environmental matrices remediation. 相似文献
China's policy on structural adjustment of its agriculture is an effective instrument for increasing agricultural production and developing the rural economy. The policy interventions used included converting cultivated land from growing grain crops to cash crops and increasing the production of meat, eggs and milk. The impacts of these changes on grain supply between 1998 and 2004 are identified and quantified at national and regional levels. The data on cultivated land diverted to other purposes are presented for the benefit of the decision maker. The paper concludes that the policy had significant impacts on China's grain supply, which declined by 14.4% of the total grain output in 1998. However, nearly 40% of the cultivated land at national level could potentially be converted by 2010. Regional disparities in the extent of the reduction in grain supply and in land conversion are highlighted with reference to different economic conditions and policy directions. 相似文献
Crassostrea ariakensis is an important aquacultured oyster species in Asia, its native region. During the past decade, consideration was given to
introducing C. ariakensis into Chesapeake Bay, in the United States, to help revive the declining native oyster industry and bolster the local ecosystem.
Little is known about the ecology and biology of this species in Asia due to confusion with nomenclature and difficulty in
accurately identifying the species of wild populations in their natural environment. Even less research has been done on the
population genetics of native populations of C. ariakensis in Asia. We examined the magnitude and pattern of genetic differentiation among 10 wild populations of C. ariakensis from its confirmed distribution range using eight polymorphic microsatellite markers. Results showed a small but significant
global θST (0.018), indicating genetic heterogeneity among populations. Eight genetically distinct populations were further distinguished
based on population pairwise θST comparisons, including one in Japan, four in China, and three populations along the coast of South Korea. A significant positive
association was detected between genetic and geographic distances among populations, suggesting a genetic pattern of isolation
by distance. This research represents a novel observation on wild genetic population structuring in a coastal bivalve species
along the coast of the northwest Pacific. 相似文献
Journal of Material Cycles and Waste Management - The reclamation of waste rigid polyurethane foam (WRPUF) is capable of realizing both circulations of waste and protection of the environment. In... 相似文献