Low nutrient levels as drinking water conditions can reduce the fitness cost of efflux pump-mediated ciprofloxacin resistance in Pseudomonas aeruginosa

The long-term persistence of antibiotic resistance in the environment, especially in drinking water, is a public health concern. Expression of an efflux pump, an important mechanism of resistance to antibiotics, usually confers a fitness cost in bacteria. In this study, we aimed to determine why antibiotic resistance conferred by overexpression of an efflux pump persisted in low-nutrient environments(TOC 10 mg/L) such as drinking and source water in which antibiotic selective pressure might be very low or even absent.Competition experiments between wild-type Pseudomonas aeruginosa and ciprofloxacinresistant mutants revealed that the fitness cost of ciprofloxacin resistance significantly decreased(p 0.05) under low-nutrient(0.5 mg/L total organic carbon(TOC)) relative to high-nutrient(500 mg/L TOC) conditions. Mechanisms underlying this fitness cost were analyzed. The mexD gene expression in resistant bacteria(cip_3 strain) was significantly lower(p 0.05) in low-nutrient conditions, with 10 mg/L TOC((8.01 ± 0.82)-fold), than in high-nutrient conditions, with 500 mg/L TOC((48.89 ± 4.16)-fold). Moreover, rpoS gene expression in resistant bacteria((1.36 ± 0.13)-fold) was significantly lower(p 0.05) than that in the wild-type strain((2.78 ± 0.29)-fold) under low-nutrient conditions(10 mg/L TOC),suggesting a growth advantage. Furthermore, the difference in metabolic activity between the two competing strains was significantly smaller(p 0.05) in low-nutrient conditions(5 and 0.5 mg/L TOC). These results suggest that nutrient levels are a key factor in determining the persistence of antibiotic resistance conferred by efflux pumps in the natural environment with trace amounts or no antibiotics.     

Transport and humification of dissolved organic matter within a semi-arid floodplain

In order to understand the transport and humification processes of dissolved organic matter(DOM) within sediments of a semi-arid floodplain at Rifle,Colorado,fluorescence excitation–emission matrix(EEM) spectroscopy,humification index(HIX) and specific UV absorbance(SUVA) at 254 nm were applied for characterizing depth and seasonal variations of DOM composition.Results revealed that late spring snowmelt leached relatively fresh DOM from plant residue and soil organic matter down into the deeper vadose zone(VZ).More humified DOM is preferentially adsorbed by upper VZ sediments,while non-or lesshumified DOM was transported into the deeper VZ.Interestingly,DOM at all depths undergoes rapid biological humification process evidenced by the products of microbial by-product-like(i.e.,tyrosine-like and tryptophan-like) matter in late spring and early summer,particularly in the deeper VZ,resulting in more humified DOM(e.g.,fulvic-acid-like and humic-acid-like substances) at the end of year.This indicates that DOM transport is dominated by spring snowmelt,and DOM humification is controlled by microbial degradation,with seasonal variations.It is expected that these relatively simple spectroscopic measurements(e.g.,EEM spectroscopy,HIX and SUVA) applied to depth-and temporally-distributed pore-water samples can provide useful insights into transport and humification of DOM in other subsurface environments as well.     

生态保护红线在实践过程中的问题剖析——以湖北宜昌为例

宜昌市生态保护红线于2015年1月正式颁布实施,文章结合宜昌市生态保护红线的应用情况,剖析了生态功能红线、水环境质量红线及大气环境质量红线在实践过程中存在的主要问题,同时,对生态保护红线管理机制需要解决的重点问题进行了阐述。     

烟气对民航安全的评估技术研究

工业设施排放的烟气对民航具有潜在影响,开展烟气对民航影响方面的研究,对维护机场净空安全具有重要意义.结合国内外烟气对民航安全评估的相关研究现状,归纳了烟气自身及其排放源的特点.确定烟气主要影响机场附近航行安全,同时烟气排放源可视为一般障碍物,并从烟气流和烟气成分角度分析烟气自身对航行安全的影响.最后对实测、软件仿真、风洞试验等现有评估方法进行了对比分析,归纳了烟气评估技术现实情况,并展望了评估技术未来的发展趋势.     

Distribution and chemical partitioning of heavy metals in marine near-shore sediment cores: a case study from the Xugou, Lianyungang, China

The concentrations and chemical partitioning of heavy metals (Co, Cr, Ni, Zn, Cu, and Pb) in the marine near-shore sediment cores were investigated. Typically, the mean concentrations from Core B sediment samples were 98.6, 21.1, 47.0, 46.4, 107.6, and 31.9 mg kg^???1 for Cr, Co, Ni, Cu, Zn, and Pb, respectively. The heavy metal concentrations were normalized to commonly used reference elements Al, Li, Sc, and total organic carbon. Based on Pearson coefficients, Li was found to be a good normalizer for Co (r?= 0.974), Cr (r?= 0.967), Ni (r?= 0.898), and Zn (r?= 0.929) in 80 sediment samples from three sampling sites. However, the correlation coefficients between Li and Cu, and Li and Pb were relatively low. Multivariate statistic approaches (Principal Component Analysis and Cluster Analysis) were adopted for data treatment, allowing the identification of two main factors controlling the heavy metal variability in the sediments. Heavy metals in the enrichment sections were evaluated by a sequential extraction method for possible chemical forms in sediments. The results showed that the residual, Fe/Mn oxides and Organic/sulfide fractions were dominant geochemical phases in the enriched sections, indicating low bioavailability of heavy metals in sediments.     

三峡库区土地自然坡度和高程对经济发展的影响

坡度和高程是土地资源固有的二个重要环境因子 ,对土地利用和土地生产力有直接作用。在山区 ,坡度和高程基本上决定了土地利用方向和利用方式 ,从而影响到经济效益和发展潜力。三峡库区面积 6 2 6 4 0km2 ,为丘陵、低中山区 ,山高坡陡。按坡度和高程对农业生产的影响程度进行分级 :0～ 7°、7～ 15°、15～ 2 5°、>2 5° ;80 0m以下 ,80 0m以上。在编制的 1:10万土地自然坡度和高程分级图及土地利用图的基础上获取各坡度、高程级及其土地利用类型和数据。 4个坡度级土地面积随坡度的增加而加大 ,随海拔的增高而减小。耕地大部分集中在 80 0m以下 7～ 15°和 15～ 2 5°的坡度上 ,优质耕地少 ,中、低产地多 ,并存在大量的陡坡耕地。森林大多分布在 80 0m以上 >2 5°陡坡上。根据对 1980～ 1996年库区主要县、市农业年产值与自然坡度和高程的定量分析比较 ,可以看出它们之间存在着明显的反比例关系。综合提高库区土地生产力 ,必须遵循“有所为 ,有所不为”原则 ,进行农业结构调整 ,不片面强调增加粮食产量而盲目扩大耕地面积 ,大力改造 15～ 2 5°的中、低产地 ,有计划、按步骤并在尊重农民意愿的基础上进行退陡坡旱地还林 (草 ) ,改善生态环境。     

城市政府建立环境管理体系的绩效分析

从城市的可持续发展和建立循环经济社会框架的需求出发．阐述了城市政府建立环境管理体系的必要性和意义；并分别从构筑沟通、监督、服务、链接四大平台．提高政府对突发事故的应急能力。建立循环经济社会框架，提高招商引资的竞争力和培养全员环境保护意识从五个方面分析论证了建立环境管理体系可以取得的环境绩效，最终做到规范政府管理，实现环境与经济，社会的协调发展。     

“限塑令”的公众行为响应与白色污染控制对策——以咸宁市为例

以咸宁市消费者人群为研究对象,通过问卷调查统计并分析了消费者对政策（塑料购物袋收费）的行为响应。与此同时根据咸宁市统计年鉴和环境公报.统计估算了城市生活垃圾及白色污染的产生量及其变化趋势,研究结果表明：政府强制措施的出台限制了白色垃圾的产生量,但公众配合意愿尚不高。在此基础上,基于系统化原理,本研究提出了城市生活垃圾及白色污染治理的对策措施。     

间接原子吸收法测定水中硫化物

通过将水样酸化后转化成硫化氢,用氮气带出,被含有定量且过量的铜离子吸收液吸收。分离沉淀后,通过测定上清液中剩余的铜离子,对硫进行间接定量。方法检出限为0.2 mg·L^-1,对实际水样进行分析,加标回收率在90%～95%之间,结果令人满意。