河流型饮用水水源保护区划分技术方法研究--以淮河淮南段某水厂为例

划定饮用水水源地保护区是保护环境、确保供水质量的有效手段。通过对国内外饮用水水源保护区划分方法的研究进程分析,以HJ/T 338—2007《饮用水水源保护区划分技术规范》为依据,通过实例研究河流型水源保护区划分技术方法。     

Cloning and expression analysis of geosmin synthase gene from Aphanizomenon gracile北大核心CSCD

Geosmin is a secondary metabolite responsible for earthy odors. The occurrence of geosmin has great impact on the quality of water environment. The gene essential for geosmin biosynthesis have been identified in several species. But little is known about the mechanism of geosmin synthesis in Aphanizomenon gracile. This study attempted to clone the gene involved in geosmin biosynthesis of Aphanizomenon gracile a nd a nalyze t he geosmin production u nder d ifferent e nvironments. T he high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR) was used to amplify the full-length of geosmin synthase gene from Aphanizomenon gracile (WH-1). Real time PCR (RT-PCR) was applied to quantify the geosmin production in different light and temperature. As a result, geo, a geosmin synthase gene from Aphanizomenon gracile (WH-1) was cloned by hiTAIL-PCR. The full-length open reading frame (ORF) of geo was 2 262 bp, coding for a protein of 753 amino acids. Meanwhile, WH-1 was treated with different environment conditions and mRNA expression levels of geo were quantified by RT-PCR. It was found that low temperature (15 °C), high light intensity (35 μmol m-2 s-1) and continuous light illumination were beneficial to the expression of geo. The successful amplification of geosmin synthase gene verified that hiTAIL-PCR is an effective and simple procedure of low cost. The result provides fundamental knowledge on the monitoring and prevention for odorants.     

基于野外数据建立大型底栖动物电导率水质基准的可行性探讨

科学合理地设定水质基准是水生生物保护和水生态系统恢复的基础。水体电导率数值的高低由溶解于其中的各种阴阳离子的浓度所决定,而较多的研究也证明电导率能够显著地影响水生生物类群,特别是大型底栖动物物种存活和群落结构的稳定性。离子组成的复杂性,导致无法开展基于室内实验的水生生物电导率基准值的推导。本文借鉴美国环境保护局基于野外调查数据建立电导率基准值的方法,采用流行病学打分方法,分别从电导率是否对大型底栖动物敏感物种存在毒性效应、人为干扰的增强是否会通过电导率产生毒性作用,以及其他环境因子是否对电导率基准值的制定产生影响3个方面,探讨了基于野外数据建立我国电导率基准的可行性。研究区域为辽河流域的浑河及太子河,野外调查数据采集于2009年8月至2010年5月。研究结果表明,电导率的升高显著降低了大型底栖动物敏感物种的出现频率。通过分析研究区域土地利用方式同电导率之间的相关性,证明了农业和城镇建设用地比例的增加显著地增加了水体中电导率的数值。对有可能引起物种消失的其他环境因子进行分析,结果表明电导率是引起大型底栖动物消失的主要原因。综合以上结果,基于野外调查数据来建立大型底栖动物电导率基准值是可行的,为我国水质基准的研究提供了新的思路和方法。     

日粮中铜胁迫对吉富罗非鱼幼鱼抗氧化、脂质过氧化及肝脏组织结构的影响

吉富罗非鱼是我国南方沿海地区水产养殖中的主要经济鱼类之一,但近年来,随着我国城镇化和工业化进程的推进,罗非鱼养殖面临着前所未有的铜富集的挑战。为探明日粮中铜胁迫对吉富罗非鱼幼鱼抗氧化系统和肝脏组织结构的影响,将1 080条罗非鱼幼鱼暴露于6个浓度梯度(0、3、30、300、1 000、3 000 mg·kg-1)的高铜日粮中,通过60 d的暴露试验,实时测定罗非鱼血清与肝脏抗氧化能力,监测肝脏病理变化。结果表明,在本试验条件下,罗非鱼幼鱼血清和肝脏中MDA的含量随日粮中铜含量的增加和胁迫时间的延长显著升高,而SOD、GSH-PX和Cu Zn-SOD的活性表现出先升高后降低的趋势;各组间肝脏表现出不同程度的病变,主要是浊肿变性和脂肪变性,且第Ⅲ、Ⅳ、Ⅴ组肝脏病变严重。综上,日粮中铜胁迫对吉富罗非鱼幼鱼的抗氧化机能有较明显的抑制作用,长时间的暴露能严重损伤其肝脏的组织结构,因此,建议吉富罗非鱼幼鱼日粮中铜的实际含量应控制在42.36 mg·kg-1以下。     

Leaching-induced migration and compositional form change of Cu,Zn, and Cd from sludge to loess

Land utilization of sewage sludge has become one of the major issues in environmental mitigation in China. This is particularly important in the Loess Plateau of Northwest China. Sludge enriched with organic matter and nutrients might effectively help to resolve the problems of silty loess soils as evidenced by porosity defect, structural alterations and absence of fertility. After sludge flows into the loess, irrigation water contains heavy metals that leach and migrate into sludge and consequently related human health risks may occur that raise concerns; and this situation needs to be rectified. The characteristics of the vertical migration and composition form change of Cu, Zn, and Cd from composted sludge to loess, and corresponding influencing factors, were investigated by performing a soil column simulation test under the leaching treatment of a one-year irrigation water capacity. Results demonstrated that: (1) composted sludge significantly improved loess fertility, and irrigation leaching transported only a small quantity of organic matter in sludge in the plough layer; (2) although some of the Cu, Zn, and Cd in composted sludge migrated to and concentrated in the middle and upper layers of the soil column during leaching, these metals were mostly retained in the plough layer; and (3) after the leaching treatment of the one-year irrigation water capacity, the compositions of Cu, Zn, and Cd (particularly Cu and Zn) in both plough layer and loess began to stabilize with low concentrations in the exudate. These findings confirmed the applicability of composted sludge in loess regions. This study provided a new insight into the sludge reuse in alkaline soils in arid and semi-arid region.     

甘肃南部典型城镇PM2.5浓度及水溶性离子特征分析

为研究甘肃南部城镇PM_2.5及水溶性离子（WSIIs）浓度水平,于2019年4月—2020年2月在甘肃成县按季度进行PM_2.5样品采集,分析了其变化特征,并运用相关和主成分分析法进行来源解析.结果表明：采样期间甘肃成县PM_2.5年平均质量浓度为（57.2±26.9） μg·m^-3,表现为冬季>春季>秋季>夏季的季节变化特征,冬季质量浓度约为夏季的1.9倍,全年空气质量优良率为81%,其中夏季达100%.WSIIs质量浓度呈现SO₄^2->NO₃^->Na⁺>NH₄⁺>Ca²⁺> K⁺>Cl^->Mg²⁺的特征.SNA是浓度水平最高的3种水溶性离子,占8种主要水溶性离子浓度的70.1%.ρ（NO₃^-）/ρ（SO₄^2-）平均值为0.6,表明工农业生产及化石燃料燃烧排放等固定源是颗粒物污染的主要来源.新型冠状病毒疫情期间人员管控对PM_2.5和水溶性离子中SNA质量浓度影响显著,PM_2.5平均质量浓度降幅达44.2%.源解析表明,PM_2.5中WSIIs主要来自化石燃料燃烧、生物质燃烧及二次源和道路建筑扬尘等.     

A novel approach to estimate and control denitrification performance in activated sludge systems with respirogram technology

Respirogram technology has been widely applied for aerobic process, however, the response of respirogram to anoxic denitrification is still unclear. To reveal such response may help to design a new method for the evaluation of the performance of denitrification. The size distribution of flocs measured at different denitrification moments demonstrated a clear expansion of flocs triggered by denitrification, during which higher specific endogenous and quasi-endogenous respiration rates (SOUR_e and SOUR_q) were also observed. Furthermore, SOUR_q increases exponentially with the specific denitrification rate (SDNR), suggesting that there should be a maximum SDNR in conventional activated sludge systems. Based on these findings, an index R_q/t, defined as the ratio of quasi-endogenous (OUR_q) to maximum respiration rate (OUR_t), is proposed to estimate the denitrification capacity that higher R_q/t indicates higher denitrification potential, which can be readily obtained without complex measurement or analysis, and it offers a novel and promising respirogram-based approach for denitrification estimation and control by taking measures to extend anoxic time to maintain its value at a high level within a certain range.     

Extracellular polymeric substance induces biogenesis of vivianite under inorganic phosphate-free conditions

Vivianite is often found in reducing environments rich in iron and phosphorus from organic debris degradation or phosphorus mineral dissolution. The formation of vivianite is essential to the geochemical cycling of phosphorus and iron elements in natural environments. In this study, extracellular polymeric substances (EPS) were selected as the source of phosphorus. Microcosm experiments were conducted to test the evolution of mineralogy during the reduction of polyferric sulfate flocs (PFS) by Shewanella oneidensis MR-1 (S. oneidensis MR-1) at EPS concentrations of 0, 0.03, and 0.3 g/L. Vivianite was found to be the secondary mineral in EPS treatment when there was no phosphate in the media. The EPS DNA served as the phosphorus source and DNA-supplied phosphate could induce the formation of vivianite. EPS impedes PFS aggregation, contains redox proteins and stores electron shuttle, and thus greatly promotes the formation of minerals and enhances the reduction of Fe(III). At EPS concentration of 0, 0.03, and 0.3 g/L, the produced HCl-extractable Fe(II) was 107.9, 111.0, and 115.2 mg/L, respectively. However, when the microcosms remained unstirred, vivianite can be formed without the addition of EPS. In unstirred systems, the EPS secreted by S. oneidensis MR-1 could agglomerate at some areas, resulting in the formation of vivianite in the proximity of microbial cells. It was found that vivianite can be generated biogenetically by S. oneidensis MR-1 strain and EPS may play a key role in iron reduction and concentrating phosphorus in the oligotrophic ecosystems where quiescent conditions prevail.     

Alterations in microbial community during the remediation of a black-odorous stream by acclimated composite microorganisms

Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediation by the acclimated complex microorganisms of a typical black-odorous stream. After the treatment, the total phosphorus and ammonia contents decreased by 74.0% and 76.3% and the concentrations of dissolved oxygen increased from 1.65 to 4.90 mg/L, indicating the effectiveness of the acclimated composite microorganisms. The proportion of Bacteroidetes decreased significantly by 48.1% and that of Firmicutes increased by 2.23% on average, and the microbial diversity index first increased and then tended to be uniform. Redundancy analysis demonstrated that the pH, dissolved oxygen, and oxidation-reduction potential together determined the composition of the microbial communities (p < 0.05). These findings showed that the acclimated composite microorganisms can effectively remediate the black odor.