柠檬酸盐络合铜在污泥上的吸附

利用二沉池污泥进行吸附柠檬酸盐络合铜实验。研究了吸附动力学、热力学、液固比以及pH值对吸附的影响,同时对比污泥吸附前后FTIR光谱推测吸附主要基团。结果表明,吸附速率受多种机制共同影响;准二级动力学模型能较好地描述污泥吸附柠檬酸盐络合铜动力学数据;增加液固比,总铜去除率下降;偏酸性环境下污泥对络合铜吸附效果较优;吸附过程符合Langmuir等温模型;红外光谱特征分析表明,污泥含有的—OH、酰胺基、—COO-、硅酸盐物质可能是污泥吸附络合铜的主要活性基团。     

气相色谱法测定气田产出水中的甲醇含量

文章建立了使用气相色谱仪分析气田产出水中甲醇含量的方法。通过实验在设定不同分流比的情况下,能有效监测出气田产出水中不同浓度的甲醇含量,此方法灵敏度高,最低检出限为0.02%,测定气田产出水中甲醇加标回收率达到93%～94%。     

高职院校计算机应用专业《JSP动态网站设计》课程改革探析

通过分析当前社会需求,结合高职高专院校目前的教学模式,提出计算机应用专业的《JSP动态网站设计》课程的教学改革方案。在基于工作任务的lAPP（导入-分析-实践-项目）教学情境中,开展“课堂实训＋虚拟公司＋校外实训基地”三位一体的实践模式教学,同时,将对工作过程的考核纳入课程的整个考核体系之中,使学生在课程中能够获得真实的职业体验和完善的实践能力。     

大学生英语口语交流障碍的心理因素及克服办法

目前大学生的英语口语表达能力总体很差,在口语交流上也出现各种障碍,其原因除学生英语学习能力较差以外,态度问题、动机偏差、缺乏自信、不耐挫折、缺乏热情、过于紧张、性格内向等心理因素也起着关键的作用。对于大学生英语口语交流存在的障碍,可以通过激发兴趣、心理暗示、教师鼓励、减少心理压力、结交外国朋友等有效方法,指导学生进行口语训练,提高学生口语水平。     

Temporal and Spatial Variability of Water Supply Stress in the Haihe River Basin,Northern China1

Ji, Yuhe, Liding Chen, and Ranhao Sun, 2012. Temporal and Spatial Variability of Water Supply Stress in the Haihe River Basin, Northern China. Journal of the American Water Resources Association (JAWRA) 48(5): 999‐1007. DOI: 10.1111/j.1752‐1688.2012.00671.x Abstract: Water resources are becoming increasingly stressed under the influence of climate change and population growth in the Haihe River Basin, Northern China. Assessing the temporal and spatial variability of water supply stress is urgently needed to mitigate water crisis caused by water resource reallocation. Water supply and use data were compiled for the time period of 1998‐2003 in this synthesis study. The Water Supply Stress Index (WSSI) as defined as Water Demand/Water Supply was used to quantitate whether water supply could meet the demand of human activities across the study region. We found a large spatial gradient of water supply stress in the study region, being much higher in the eastern subbasins (ranging from 2.56 to 4.31) than the west subbasins (ranging from 0.56 to 1.92). The eastern plain region not only suffered more serious water supply stress but also had a much higher interannual variability than the western hilly region. The uneven spatial distribution of water supply stress might result from the distribution of land use, population, and climate. Future climate change and rapid economic development are likely to aggravate the existing water crisis in the study region.     

Anaerobic degradation of microcrystalline cellulose: kinetics and micro-scale structure evolution

The degradation kinetics and micro-scale structure change of microcrystalline cellulose during anaerobic biodegradation were investigated. A modified Logistic model was established to properly describe the kinetics, which showed good fitness and wide applicability for cellulose degradation. A maximum degradation rate of 0.14 g L⁻¹ h⁻¹ was achieved after cultivating for 51.5 h. This result was in good agreement with the scanning electron microscope and X-ray diffraction analysis. Channels of 400-500 nm size started to occur on the crystalline surface of cellulose at around the inflexion time. Accordingly, the crystallinity significantly decreased at this point, indicating a degradation of the crystalline structure zones by anaerobic bacteria. This study offers direct morphological evidence and quantitative analysis of the biodegradation process of cellulose, and is beneficial to a better understanding of the cellulose degradation mechanism.     

Treatment of coking wastewater by an advanced Fenton oxidation process using iron powder and hydrogen peroxide

In this study the treatment of coking wastewater was investigated by an advanced Fenton oxidation process using iron powder and hydrogen peroxide. Particular attention was paid to the effect of initial pH, dosage of H₂O₂ and to improvement in biodegradation. The results showed that higher COD and total phenol removal rates were achieved with a decrease in initial pH and an increase in H₂O₂ dosage. At an initial pH of less than 6.5 and H₂O₂ concentration of 0.3 M, COD removal reached 44-50% and approximately 95% of total phenol removal was achieved at a reaction time of 1 h. The oxygen uptake rate of the effluent measured at a reaction time of 1 h increased by approximately 65% compared to that of the raw coking wastewater. This indicated that biodegradation of the coking wastewater was significantly improved. Several organic compounds, including bifuran, quinoline, resorcinol and benzofuranol were removed completely as determined by GC-MS analysis. The advanced Fenton oxidation process is an effective pretreatment method for the removal of organic pollutants from coking wastewater. This process increases biodegradation, and may be combined with a classical biological process to achieve effluent of high quality.     

Cornmeal-induced resistance to ciprofloxacin and erythromycin in enterococci

Triclosan is used as an antibacterial agent in household items and personal care products. Since this compound is found in maternal milk of humans and bodies of wild animals, there is growing concern among some consumer groups and scientific community that triclosan is adverse for humans and wild animals. In order to estimate adverse actions of triclosan, the effects of triclosan on intracellular Zn²⁺ concentration and cellular thiol content were studied in rat thymocytes by the use of flow cytometer with appropriate fluorescent probes. Triclosan at 1-3 μM (sublethal concentrations) increased the intensity of FluoZin-3 fluorescence (intracellular Zn²⁺ concentration) and decreased the intensity of 5-chloromethylfluorescein (5-CMF) fluorescence (cellular thiol content). Negative correlation (r = −0.985) between triclosan-induced changes in FluoZin-3 and 5-CMF fluorescences was found. Removal of external Zn²⁺ did not significantly affect the triclosan-induced augmentation of FluoZin-3 fluorescence, suggesting an intracellular Zn²⁺ release by triclosan. These actions of triclosan were similar to those of H₂O₂ and triclosan significantly potentiated the cytotoxicity of H₂O₂. Therefore, the results may suggest that triclosan at sublethal concentrations induces oxidative stress that decreases cellular thiol content, resulting in an increase in intracellular Zn²⁺ concentration by Zn²⁺ release from intracellular store(s). Since recent studies show many physiological roles of intracellular Zn²⁺ in cellular functions, the triclosan-induced disturbance of cellular Zn²⁺ homeostasis may induce adverse actions on the cells.     

Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China

Increasing antibacterial resistance and pathogenicity in the environment is of growing concern due to its potential human risk. In the present study, 236 Escherichia coli isolates were collected from Wenyu River in China on drugless (48 isolates) and quinolone-containing plates (189 isolates). Their minimum inhibitory concentrations (MICs) were determined ranging from 0.125 μg mL⁻¹ to 128 μg mL⁻¹. Mutation points related to fluoroquinolone resistance were observed at S83 to L and D87 to N or Y in the GyrA subunit and S80 to R or I and E84 to G in the ParC subunit. Generally, MICs of LEV and GAT are dependent on the patterns of these mutation points. The profile with three mutation points was related to LEV-resistant E. coli isolates, and the (S83L, D87N + S80I) mutation profile was most prevalent (65.7%) in LEV-resistant isolates, while a large proportion of isolates, even those with three mutation points, were susceptive to GAT. The incidence of virulence factors in LEV-resistant isolates (44.7%, 59/132) was much higher than in nonresistant isolates (23.1%, 24/104) (χ² = 11.925, 1° of freedom, p < 0.001) indicating that fluoroquinolone-resistant E. coli would pose a potential risk. A similar distribution was also found in isolates resistant to GAT (χ² = 7.843, 1° of freedom, p = 0.0079).     

Catalytic destruction of pentachlorobenzene in simulated flue gas by a V2O5-WO3/TiO2 catalyst

Pentachlorobenzene (PeCB) in simulated flue gas was destructed by a commercial V₂O₅-WO₃/TiO₂ catalyst in this study. The effects of reaction temperature, oxygen concentration, space velocity and some co-existing pollutants on PeCB conversion were investigated. Furthermore, a possible mechanism for the oxidation of PeCB over the vanadium oxide on the catalysts was proposed. Results show that the increase of gas hourly space velocity (GHSV) and the decrease of operating temperature both resulted in the decrease of PeCB removal over the catalyst, while the effect of the oxygen content in the range of 5-20% (v/v) on PeCB conversion was negligible. PeCB decomposition could be obviously affected by the denitration reactions under the conditions because of the positive effect of NO but negative effect of NH₃. The introduction of SO₂ caused the catalyst poisoning, probably due to the sulfur-containing species formed and deposited on the catalyst surface. The PeCB molecules were first adsorbed on the catalyst surface, and then oxidized into the non-aromatic acyclic intermediates, low chlorinated aromatics and maleic anhydride.