Purification and some properties of β-N-acetyl-D-glucosaminidase from prawn (<Emphasis Type="Italic">Penaeus vannamei</Emphasis>)

The -N-acetyl-D-glucosaminidase (NAGase, EC 3.2.1.52) from prawn (Penaeus vannamei) was purified by extraction with 30% ethanol solution and ammonium sulfate fractionation, then chromatographed on Sephadex G-100 followed by DEAE-cellulose (DE-32) columns. The purified enzyme determined to be homogeneous by polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The specific activity of the purified enzyme was 1,560 U mg^–1. Enzyme molecular weight was determined to be 105,000 Da; it contained two subunits of the same mass (45,000 Da). The pI value was calculated to be 4.8 by isoelectric focusing. The optimum pH and optimum temperature of the enzyme for the hydrolysis of pNP--D-GlcNAc (enzyme substrate) were determined to be pH 5.2 and 45°C, respectively. The behavior of the enzyme during hydrolysis of pNP--D-GlcNAc followed Michaelis–Menten kinetics, with K_m=0.254 mM and V_m=9.438 M min^–1, at pH 5.2 and 37°C. The stability of the enzyme was investigated, and the results showed that the enzyme was stable in a pH range from 4.2 to 10.0 and at temperatures <40°C. The effects of metal ions on the enzyme were also studied. Li⁺, Na⁺ and K⁺ had no influence on enzyme activity. Mg²⁺, Ca²⁺ and Mn²⁺ activated the enzyme, while Ba²⁺, Zn²⁺, Co²⁺, Cd²⁺, Hg²⁺, Pb²⁺ Cu²⁺, Fe³⁺ and Al³⁺ showed various degrees of inhibitory effects on the enzyme.Communicated by O. Kinne, Oldendorf/Luhe     

普通小球藻和铜绿微囊藻与粘土颗粒凝聚沉降行为研究

本研究结果表明,不同的藻类和不同的粘土颗粒均使藻类与粘土颗粒的凝聚沉降发生变化。结果还表明,溶液中[Cu~(2+)]浓度超过10~(-5)mol后,加快了小球藻与高龄土的凝聚沉降。使80％以上的小球藻和高岭土在不到10min内即凝聚沉降。在最后结合实验结果讨论了利用藻类与粘土的凝聚沉降治理水体污染。     

Compositional Limitations in Poly–3–Hydroxyalkanoates Produced by Pseudomonas oleovorans

It is well known that Pseudomonas oleovorans can utilize sodium octanoate for both cell growth and the synthesis of polyhydroxyalkanoates (PHAs), but it can utilize sodium butyrate only for limited cell growth and not for the polyester formation when this substrate is the sole carbon source. Therefore, these two substrates were evaluated as cofeeds for the possible incorporation of 3-hydroxybutyryl groups in the resulting PHA. When sodium butyrate and sodium octanoate were fed to P. oleovorans as cosubstrates in various proportions, the resultant cell density and polymer content were proportional to the amount of sodium octanoate in the feed. The PHA extracted from cells grown in all combinations of these cosubstrates had similar unit compositions of approximately 8 mole % 3-hydroxyhexanoate, 91 mole % 3-hydroxyoctanoate and 1 mole % 3-hydroxydecanoate. 3-Hydroxybutyrate units were not detected in any of the PHAs isolated, indicating that these units could not be incorporated in the copolymer synthesized by P. oleovorans either because the cell did not synthesize that monomer or, if it did, the PHA synthase could not copolymerize it with the longer chain monomers.     

水处理无机混凝剂的研究进展

概略评述了国内外无机混凝剂的研究进展。无机混凝剂可分为铝盐类、铁盐类、铁铝复合类、活化硅酸等,聚合氯化铝（简称ＰＡＣ）是目前水处理中广泛应用的无机高分子混凝剂。无机复合类混凝剂是近来年发展十分迅速的水处理剂,聚硅酸铝盐类是其中的代表。文中对无机混凝剂今后的研究工作提出了建议,认为在无机混凝剂的研制开发中,要加强应用基础性研究,产品多样化、专门化,要降低生产成本,提高产品质量,进行优化研究。     

城市污水厌氧处理技术研究进展

通过介绍厌氧处理技术在城市污水处理中的现状及研究焦点,论述了以微生物固定化和提高污泥与污水混合效率为基础的一系列高速厌氧反应器的特点,分别阐述了升流式厌氧污泥床、折流式厌氧反应器、内循环厌氧反应器及颗粒污泥膨胀床反应器的研究进展,提出高效率的厌氧处理系统应满足的条件,并与好氧处理技术作了对比,综述了新型反应器的研制及其组合工艺的应用在废水处理领域所发挥的重要作用.     

影响微生物絮凝剂生成的条件研究

从污水中筛选到了一株絮凝活性较好的絮凝剂产生菌 ,分别研究了碳源、氮源、供氧量、培养温度等条件对其产絮凝剂的影响。结果表明 ,葡萄糖、淀粉是较好的碳源 ,蛋白胨等有机氮作氮源较好 ,供氧量较大有利于产絮凝剂 ,最适宜培养温度为 30℃ ,最佳pH为 6 .5～ 7.5。     

我国不锈钢制品与铝制品行业进展与趋势

对不锈钢制品和铝制品行业的进展进行了分析 ,并对其发展趋势进行了预测     

福建中奥陶世——晚侏罗世地层的古地磁学研究

福建古生界至中生界部分地层古地磁研究认为,华南地块的主体部分首先于中奥陶世时期即已拼合,作为一个整体由南纬23.8°向北移动,二叠纪之后越过赤道,继续北移,直至侏罗纪末到达北纬19.6°的位置.而华南地块同扬子地块的拼合是在加里东末期开始的,至少可以认为是在早石炭纪之前两地块就靠近了。这一时期,华南地块和扬子地块的地质构造运动十分活跃.