Growth inhibition and formation of morphologically abnormal cells of <Emphasis Type="Italic">Akashiwo sanguinea</Emphasis> (Hirasaka) G. Hansen et Moestrup by cell contact with <Emphasis Type="Italic">Cochlodinium polykrikoides</Emphasis> Margalef

We investigated the growth interaction between Cochlodinium polykrikoides and Akashiwo sanguinea using bi-algal cultures. When cultured together, morphologically abnormal cells of A. sanguinea appeared and the growth of A. sanguinea notably decreased. When C. polykrikoides and A. sanguinea were cultured separately in inner and outer wells separated by a membrane with a pore size of 3 μm, neither species showed suppressed growth and no morphologically abnormal cells of A. sanguinea were observed. Furthermore, filtrates from C. polykrikoides cultures did not affect the cell morphology or the growth of A. sanguinea. When the abnormal cells of A. sanguinea were transferred from bi-algal cultures into fresh medium and cultured as a mono-algal culture, cells recovered their normal morphology and multiplied. Therefore, the growth inhibition and formation of morphologically abnormal cells of A. sanguinea would be induced by constant cell contact with C. polykrikoides.     

生态住宅的水环境规划:I. 水循环

生态住宅已成为住宅建设的发展趋势和潮流,水环境是生态住宅小区的重要组成部分。文章以中信保利达美景度假区为实例,根据度假区建筑总体规划方案,结合当地自然条件和水环境现状,探讨生态住宅小区的水环境规划,全面统一规划住宅小区内各种水系统,提出小区水环境总体规划方案,充分发挥各系统的功能,使其相互衔接、协调与补充。重点对项目所在区域开发前后的水量平衡,水库的水位变化及其安全性,极端气候条件下的库区水量变化及其因应对策,高尔夫球道的用水方案以及项目开发后的水循环模型做了深入探讨,旨在为生态住宅的水环境建设提供范例。     

Styrene dimers and trimers affect reproduction of daphnid (Ceriodaphnia dubia)

The endocrine disruptor activity of styrene in humans and other vertebrates appears to be negligible. However, offspring numbers were reduced in Ceriodaphnia dubia bred in polystyrene cups. Styrene dimers and trimers were found to be eluted from the polystyrene cups by hexane and methanol with gas chromatography-mass spectrometry. Styrene dimers and trimers at concentrations of 0.04-1.7 microg/l affected C. dubia fertility (25% reduction after seven days), suggesting that styrenes have the potential to impair crustacean populations in the aquatic environment.     

Bacterial communities in different locations, seasons and segments of a dairy wastewater treatment system consisting of six segments

A dairy wastewater treatment system composed of the 1st segment (no aeration) equipped with a facility for the destruction of milk fat particles, four successive aerobic treatment segments with activated sludge and a final sludge settlement segment was developed. The activated sludge is circulated through the six segments by settling sediments (activated sludge) in the 6th segment and sending the sediments beck to the 1st and 2nd segments. Microbiota was examined using samples from the non-aerated 1st and aerated 2nd segments obtained from two farms using the same system in summer or winter. Principal component analysis showed that the change in microbiota from the 1st to 2nd segments concomitant with effective wastewater treatment is affected by the concentrations of activated sludge and organic matter (biological oxygen demand [BOD]), and dissolved oxygen (DO) content. Microbiota from five segments (1st and four successive aerobic segments) in one location was also examined. Although the activated sludge is circulating throughout all the segments, microbiota fluctuation was observed. The observed successive changes in microbiota reflected the changes in the concentrations of organic matter and other physicochemical conditions (such as DO), suggesting that the microbiota is flexibly changeable depending on the environmental condition in the segments. The genera Dechloromonas, Zoogloea and Leptothrix are frequently observed in this wastewater treatment system throughout the analyses of microbiota in this study.     

鸭绿江(丹东段)水污染控制系统规划研究

鸭绿江是中朝两国的界河,是沿江两国人民生活和生产的主要水源。下游河道长233km,可分为各具特点的三个江段:上段由浑江至水丰电站,为水库淹没区;中段由太平湾电站至马市为河流段;下段由马市至江海分界线为感潮河口段,该段长约55km,潮流界在东尖头处,距江口27km。下段中,由大沙河口开始承纳中朝两国沿岸工矿、城镇排放的大量污水,使宽阔(干流宽度为1～3km)江面的沿岸两侧形成了二条明显的岸边污染带,中方江桥处污染带宽达200m左右。从目前和未来都不能满足水产养殖和旅游的要求。本研究的目的是为经济有效地改善鸭     

甘-宁断面多回联络线停电对系统稳定性的影响

针对目前宁夏电网存在甘-宁断面多回联络线停电检修的现状,通过对甘-宁断面三回联络线进行停电方式下系统的热稳定、静态稳定、暂态稳定、动态稳定和小干扰稳定仿真计算分析,确定甘-宁断面在薄弱方式下对系统的稳定性影响。结果表明:甘-宁断面在多回联络线停电检修方式下对系统的稳定性影响较大,需对甘-宁断面采取预控措施,防止热稳定和动态稳定问题的发生。     

Mutagenicity of polyaromatic hydrocarbons by chemical models for cytochrome P450 in Ames assay

DNA damage is an important step in carcinogenesis. The Ames assay is a short-term screening of carcinogens that induce DNA damage. Most carcinogens require enzymatic activation through oxidation by cytochrome P450 (CYP450) in the presence of S9 mix. A combination of iron (Fe)(III) porphyrin and an oxidant is also able to oxidize compounds as an alternative metabolic pathway to CYP450. Previously it was reported that a chemical model containing a water-soluble 5,10,15,20-tetrakis(1-methylpyridinium4-yl)porphyrinatoiron(III) chloride (4-MPy) and tert-butyl hydroperoxide (t-BuOOH) activated aromatic amines and amides. In this study, a chemical model composed of an Fe porphyrin, water-insoluble 5,10,15,20-tetrakis(pentafluorophenyl)porphyrinatoiron(III) chloride (F₅P) or water-soluble 4-MPy was optimized with an oxidant – t-BuOOH, magnesium monoperoxyphthalate (MPPT), or iodosylbenzene (PhIO). Subsequently the mutagenicity of benzo[a]pyrene (B[a]P) and chrysene in Salmonella typhimurium TA strains was compared. B[a]P was activated by a combination of F₅P or 4-MPy plus MPPT or PhIO in S. typhimurium TA1538. The B[a]P-induced mutagenicity with F₅P plus oxidant was higher than 4-MPy plus oxidant. Mutagenicity of chrysene, a tetracyclic aromatic hydrocarbon, was not detected in the presence of F₅P/PhIO in S. typhimurium TA98, but was activated in the presence of F₅P/MPPT. The F₅P/MPPT activated other polyaromatic hydrocarbons (PAH) in the S. typhimurium TA98 assay including dibenz[a,c]anthracene, dibenz[a,h]anthracene, 3-methylcholanthrene, and benzo[a]anthracene. The results indicated that the F₅P/MPPT was the most efficient model for detecting PAH-induced mutagenicity in the Ames assay.     

Quantitative assessment of the Japanese “local production for local consumption” movement: a case study of growth of vegetables in the Osaka city region

This study aimed to assess Japan’s recent “local production for local consumption” (LPLC) movement, with a special focus on vegetables in the Osaka city region of central Japan. After collecting statistics and spatial data, we conducted a multi-scale analysis of vegetable production and consumption along with the associated energy consumption, using geographical information system software at three spatial scales along the vegetable flow paths: national, regional, and local. Vegetables consumed in the Osaka city region came from prefectures throughout Japan, and we mapped the foodshed within this region at 1-km spatial resolution, as well as the distribution of farmland, farmers’ and other markets. We also conducted a scenario analysis for reduced energy consumption through organic farming and the utilization of abandoned farmland near a city to replace food imports from distant areas. We found that the large majority of vegetables consumed in the Osaka city region currently come from remote prefectures, and that this is associated with a high level of energy consumption. Inside the Osaka city region, peri-urban vegetable farming contributes to regional vegetable provision, resulting in an approximately 70 % production/consumption ratio within an 80-km radius of the urban center; if all of the area of abandoned farmland were restored to production, this ratio would increase to approximately 75 %. Organic farming activities that bring together farmers and urbanites are emerging in many parts of the study area, contributing to increased LPLC. Scenario analysis suggested that a decrease of more than 1 × 10⁶ GJ of energy inputs could be achieved through wider adoption of local organic farming for local consumption and complete utilization of abandoned farmland in the Osaka city region.     

Influence of physical properties of ammonium nitrate on the detonation behaviour of ANFO

In order to obtain a better understanding of the non-ideal detonation behaviour of ammonium nitrate based explosives, detonation velocities of ANFO (ammonium nitrate and fuel oil) prepared with different kinds of ammonium nitrate (AN) were measured in steel tubes. In this series of test six kinds of AN were used and the influence of the pore diameter, the pore volume and the particle diameter of the AN particle on the detonation velocity of ANFO was investigated.

It was found that the pore diameter and the pore volume had a strong influence on the detonation velocities of ANFO. In the case of ANFO samples which were prepared with AN that had the same pore diameter and the pore volume, when tested the highest detonation velocity (3.85 km/s) was observed when the smallest particle diameter (<0.85 mm) was used. This value corresponded to 75% of the ideal detonation velocity, which was theoretically predicted by the CHEETAH code with the JCZ3-EOS.

The 12 months aging showed the change of the detonation velocities of ANFO and the reaction of ANFO was influenced both by the physical and the chemical properties of AN particles and oil during the storage period.