Special Issue: Statistical mathematics for ecological and environmental data

Environmental and Ecological Statistics -     

Hydrostatic pressure-induced apoptosis on nauplii of Calanus sinicus

The effect of hydrostatic pressure on embryonic development of the calanoid copepod Calanus sinicus was studied. Differences of pressure effect among blastomere stages, 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, blastula and limb-bud stage, were examined under two pressurizing conditions, abruptly (10 atm/min) and gradually (0.1 atm/min) increasing. Egg hatching success, deformity frequency and apoptotic cell degradation of hatched nauplii were examined. Egg hatching success rate was not significantly different between blastomere stages, and also between pressurizing conditions. Deformity frequencies of hatched nauplii were low in the early 1-cell and 2-cell stages, and high in the later blastula and limb-bud stages, in both abrupt and gradual pressurizing conditions. On the other hand, in regard to difference in pressurizing conditions, deformity frequency in gradual pressurizing was significantly higher than that in abrupt pressurizing rate. Gradual pressure increase seems to be more harmful to C. sinicus eggs than abrupt pressure change. Apoptosis induced in nauplii by hydrostatic pressure was detected for the first time in marine zooplankton. The embryos of C. sinicus are sensitive to pressure variations, that is, these embryos are supposed to sink to deeper waters, incurring greater risk of having deformities. In the field, C. sinicus ascend to the surface and spawn at night. By looking from this upward behavior, eggs are spawned at lower pressure and warmer temperature. Probably, the harmless low pressure and warm temperature lead eggs to hatch early and to recruit successfully.     

Hydrostatic pressure-induced apoptosis on nauplii of Calanus sinicus

The effect of hydrostatic pressure on embryonic development of the calanoid copepod Calanus sinicus was studied. Differences of pressure effect among blastomere stages, 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, blastula and limb-bud stage, were examined under two pressurizing conditions, abruptly (10 atm/min) and gradually (0.1 atm/min) increasing. Egg hatching success, deformity frequency and apoptotic cell degradation of hatched nauplii were examined. Egg hatching success rate was not significantly different between blastomere stages, and also between pressurizing conditions. Deformity frequencies of hatched nauplii were low in the early 1-cell and 2-cell stages, and high in the later blastula and limb-bud stages, in both abrupt and gradual pressurizing conditions. On the other hand, in regard to difference in pressurizing conditions, deformity frequency in gradual pressurizing was significantly higher than that in abrupt pressurizing rate. Gradual pressure increase seems to be more harmful to C. sinicus eggs than abrupt pressure change. Apoptosis induced in nauplii by hydrostatic pressure was detected for the first time in marine zooplankton. The embryos of C. sinicus are sensitive to pressure variations, that is, these embryos are supposed to sink to deeper waters, incurring greater risk of having deformities. In the field, C. sinicus ascend to the surface and spawn at night. By looking from this upward behavior, eggs are spawned at lower pressure and warmer temperature. Probably, the harmless low pressure and warm temperature lead eggs to hatch early and to recruit successfully.     

Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris

 The ability to introduce and express foreign genes can greatly facilitate in vitro studies of the molecular biology of an organism. We report here the first use of modified retroviral vectors to achieve foreign gene expression in primary cultured cells from the Oka organ and ovary of the blue shrimp Penaeus stylirostris (Litopenaeus) stylirostris (Holthuis 1980). Of the heterologous promoters tested, the Moloney murine leukemia virus and Rous sarcoma virus promoters mediated the highest levels of reporter gene expression. Firefly luciferase and Escherichia coliβ-galactosidase were successfully used as reporter genes in cultured cells from both Oka organ and ovary. These in vitro systems can be exploited for the study of shrimp cellular biology and regulation of gene expression. Received: 16 January 2000 / Accepted: 21 June 2000     

Trend analysis of machinery safety for globalization: From the Japanese point of view

In Japan, the work of translation to JIS faced many obstacles. Support groups in Western countries have submitted comments on technical problems. The movement to standard harmonization in safety has rapidly developed in international standardization. The movement for global standardization is developing throughout all the economic areas of the EU. The aim of the Machine Directive that comes into effect in stages is to provide an international formula for a type of qualification in machine safety. EN292 is a nucleus for related standards, which are like a group of planets in a universe. It is a European standard as well as a guideline for implementation, showing practical safety requirements for machines. Horizontal standards, such as EN954 and IEC61508, apply in actual practice.     

A DFT study on the carbamates formation through the absorption of CO2 by AMP

DFT calculations in gas and aqueous solution phases have been performed to study the mechanism of carbamate formation by the absorption of CO₂ in 2-amino-2-methyl-1-propanol (AMP). The results reveal the importance of considering the effect of water as solvent for the reaction to proceed. Furthermore water molecules play an important role as a basic reactant leading to stable intermediates formation. These results point at a single-step, third order reaction as the most probable mechanism for the formation of carbamate by the absorption process.     

Municipal solid waste management and short-term projection of the waste discard levels in Taiwan

Industrialization and urbanization result in significant changes in lifestyle. These lifestyle changes seem to lead to unsustainable consumption patterns and increase the generation of various kinds of environmental loads, especially the amount of municipal solid waste (MSW). Taiwan is a small island with scarce natural resources. The economic development in Taiwan has resulted in the generation of large amounts of MSW. As a result, the Taiwan Environmental Pollution Administration (TEPA) has produced regulations for waste minimization and has imposed several important policy measures that have successfully reduced the MSW discard rate in recent years and have established a public recycling network as a part of the MSW collection. Nowadays, the objective of the MSW policies in Taiwan is to develop a “zero-waste society.” This article aims to review the MSW management progress in Taiwan and to project future MSW discards up to 2011 based on the national plan and assumed scenarios for socioeconomic variables. According to the analysis results, a more sustainable consumption pattern can be proposed and the corresponding MSW management system can be planned so as to develop a low-waste-discard society.     

Three-Dimensional Modeling of an Urban Park and Trees by Combined Airborne and Portable On-Ground Scanning LIDAR Remote Sensing

In this study, we confirmed the utility of airborne and portable on-ground scanning light detection and ranging (LIDARs) for three-dimensional visualization of an urban park and quantification of biophysical variables of trees in the park. The digital canopy height model (DCHM) and digital terrain model generated from airborne scanning LIDAR data provided precise images of the ground surface and individual tree canopies. The heights of 166 coniferous and broadleaf trees of 11 species in the park were estimated from the DCHM images with slight underestimation (mean error = −0.14 m, RMSE = 0.30 m). Portable on-ground scanning LIDAR provided images of individual trees with detailed features. Tree height and trunk diameter were estimated to be within 0.31 m and 1 cm, respectively, from the on-ground LIDAR images. We combined airborne and on-ground LIDAR images to overcome blind regions and created a complete three-dimensional model of three standing trees. The model allowed not only visual assessment from all viewpoints but also quantitative estimation of canopy volume, trunk volume, and canopy cross-sectional area.     

Ozone and/or Water Stresses Could have Influenced the Betula ermanii Cham. Forest Decline Observed at Oku-Nikko, Japan

A serious forest decline of Betula ermanii Cham. has been observed at Mt. Mae-Shirane, Oku-Nikko, Japan, where high ozone (O₃) concentration and severe water deficiency have been measured. In order to consider the possibility whether O₃ and/or water stresses could have been the causes of the forest decline of B. ermanii, plant growth experiments were conducted in environment-controlled growth cabinets. Two-year-old seedlings of B. ermanii were exposed to either charcoal-filtered air (O₃ concentration <5 ppb) or 50 ppb O₃ (daily average, ranging between 20–100 ppb) for 123 days at 20.0/12.5 ± 1.0°C (day/night) and 70/80 ± 7% relative humidity (day/night). Simultaneously, seedlings were treated with three watering regime: 1.0 < pF < 1.8 (no water stress), 1.8 < pF < 2.5 (mild water stress) or 2.5 < pF < 3.0 (severe water stress). O₃ exposure significantly reduced the dry weights of leaf, root and the whole plant, while water stress significantly reduced the dry weights of each organ and the whole plant. Significant reductions of net photosynthesis, transpiration and stomatal conductance were also observed under O₃ and/or water deficiency treatments, while contents of RuBP carboxylase/oxygenase (Rubisco), chlorophyll _a+b and some essential nutrient elements (N, P, K, Mg and Ca) were not markedly changed. It was suggested that the decrease in net photosynthetic rate induced mainly by stomatal closure was the major cause of the growth reduction under O₃ and/or water stresses. No significant interactions between O₃ and water stresses were observed in terms of the depression of dry matter production, which suggested that simultaneous stress treatments of O₃ exposure and water deficiency could affect the tree growth of B. ermanii additively.