Prenatal detection of an Arg→Ter mutation at codon 111 of the pah gene using DNA amplification

A CGA→TGA mutation at codon 111 in exon 3 of the phenylalanine hydroxylase (PAH) gene was recently identified in a Chinese phenylketonuria (PKU) patient. This paper reports the prenatal diagnosis of a Chinese fetus at risk for PKU using DNA amplification with PCR and oligonucleotide hybridization. RFLP analysis revealed that the fetus had inherited a PKU gene from his mother, but his paternal PAH gene was uninformative. PCR amplification of 300 bp which included exon 3 plus the flanking intronic sequences of the PAH gene was performed. The amplified DNA was hybridized with a pair of allele-specific oligonucleotide probes. The results indicated that the fetal DNA carried a PAH 111 Arg→Ter mutant gene inherited from his father. Thus, the fetus was predicted to be affected with PKU.     

石化企业资产交易活动中的环保资产评估

环保资产评估是石化企业资产评估的重要组成部分，涉及资产所有者、经营者和使用者的合法权益，应当加以关注。借鉴现有资产评估的方法，提出了企业资产交易活动中有关环保资产评估的几个基本概念、主要内容、原则、范围、方法和标准，并做了简要说明。     

人工湿地污水处理技术及其发展现状

人工湿地与传统的污水处理法相比具有基建、运行费用低，操作与维护简单等优点。总结了人工湿地系统的研究现状和目前存在的问题，并指出了其未来的发展方向。     

LS系列硫磺回收催化剂工业装置使用总结

对比总结了LS系列催化剂的使用情况，结果表明，在一级转化器均装填LS-931Al2O3基催化剂的前提下，二级转化器上部装填LS－971脱氧保护型催化剂，下部装填LS－811Al2O3催化剂后，与单纯使用LS－931催化剂相比，单器转化率由64．1％提高到70．1％，有机硫水解率由96％提高到接近100％，两项合计使装置总硫转化率由95．1％提高到96．2％，若与两个转化器全部使用LS－811催化剂相比，则装置总硫转化率可由94．49％提高到96．2％使用效果更加显著。     

特非那丁格氏工序的清洁生产

采用合理工艺，使生产过程废物最少化是清洁生产的主要内容之一，在分析特非那丁格氏工序污染物产生的基础上，通过改进工艺，将过去排放的有机溶媒回收套用，减少污染物排放的同时，增加了经济效益。     

环境质量评价的主成分因子分析的可视化方法

环境质量评价的主成分因子分析涉及十分复杂的矩阵运算和评价成果图绘制,用Excel加Visual Basic强有力的界面设计功能和MATLAB的强大的计算和图形处理功能,通过在MATLAB中提供的一个Excel Link插件,可以直接在Excel环境下实现环境质量评价中复杂的数据处理和图表的制作功能.     

A synergetic model for implementing an integrated management system: an empirical study in China

The implementation and certification of quality, environmental and occupational health and safety management systems have become a priority for many organizations. They are viewed as symbol for success and prerequisite for survival. However, it has been proved to be difficult to operate multiple parallel management systems covering quality, environment, and occupational health and safety and to ensure their alignment with the organization's strategy. To investigate the current status of integrated management system (IMS), a structured questionnaire survey was administrated. It is concluded that the major problems for enterprises to operate multiple parallel management systems include: it causes complexity of internal management, it lowers management efficiency, it incurs cultural incompatibility, it causes employee hostility, and increases management costs. The survey also examined the internal and external factors that affect the implementation of IMS. The internal factors include: (1) human resources, (2) organizational structure, (3) company culture, and (4) understanding and perception. The external factors consist of: (1) technical guidance, (2) certification bodies, (3) stakeholders and customers, and (4) the institutional environment. The article proposes a multi-level synergy model (strategic synergy, organizational structural-resource-cultural synergy, and documentation synergy) for an effective implementation of IMS.     

土壤对煤矸石填充时地下水中F-、SO42-的吸附规律

通过室内静态模拟浸泡试验,文章探讨了平煤集团十二矿附近的土壤对该矿煤矸石井下填充过程中地下水溶出的F-和SO42-的吸附性能.研究发现:土壤对F-吸附效果较好,而对SP42-较差;改性土壤对F-和SO42-的吸附效果均较好,均能使其在地下水中的含量达到地下水Ⅲ类标准;F-和SO42-在改性土壤上的吸附等温模型分别符合Freundlich、Temkin等温式;改性土壤对F-和SO42-的解吸过程分为快速和慢速两个反应阶段.通过试验数据分析得出:井下填充时煤矸石和改性土壤的最佳配比为1∶0.26.     

生物早期警报系统在水和废水水质评价中的应用

生物监测是评价环境质量的重要手段，生物早期警报系统根据活生物对污染物的灵敏反应，快速确认污染事件，本文对生物早期警报系统的发展，设计与应用进行探讨。     

环境补偿制度——解决环境问题的经济手段之一

为了保护环境，使环境资源可持续利用，应该建立环境补偿制度。环境补偿制度是解决环境问题的有效手段之一。从环境补偿的概念出发，对环境补偿制度的理论基础进行了探讨，为我国建立环境补偿制度提供依据。