Study on nitrile-degrading microorganisms

Two strains of bacteria were isolated from nitrile polluted soils, and identified as Corynebacterium boffmanii and Arthrobacter flavescens. Acetonitrile, propionitrile, butyronitrile and acrylonitrile were degraded by these bacteria to yield corresponding amides, carboxylic acids and ammonia. The nitrile-degrading abilities of these strains were investigated. The removal rates for the nitrile were nearly 100%, after these bacteria were grown in medium containing 10000 ppm of aceto-, propio-, or butyronitrile at 28 ℃ for 24h. When the reaction mkture consisting of 5000 ppm of above mentioned nitriles or acrylonitrile and 20g (dry cell) /L resting cells of the two strains in 0.06mol/L phosphate buffer (pH7.5) was incubated separately at 25 ℃ with moderate shaking for 15 or 45 min, the nitrile could be degraded completely. The optimum growth conditions for C.hoffmanii and A.flavescens were studied as well.     

汽车覆盖件冲压工艺设计KBE系统中的知识表示技术

根据汽车覆盖件冲压工艺设计的特点 ,阐述了与汽车覆盖件冲压工艺设计有关的不同类型知识的表示方法 ,主要有一阶谓词逻辑、语义网络、产生式规则、框架和剧本、可视化文本和图象 ,以及面向对象的方法等 ,并比较了各种方法的优缺点。面向对象的集成知识表示等方法成功地应用于汽车覆盖件冲压工艺设计KBE系统的知识表示     

关于高校环境类专业本科教学的思考

论述了从培养目标、课程设置、教学方法与实践等方面培养高素质复合型的环境类专业的本科生，以满足社会需求，并为环境类专业本科教学改革提供参考。     

石材废水集约化处理工程实例

介绍了一个石材废水集约化处理的成功实例，分析、总结了混凝沉淀法处理石材废水的可行性及注意事项，并通过比较得出石材废水集约化处理较分散治理具备明显优势的结论。     

佳木斯台形变观测对东北地区几次浅震“场兆”异常的探讨

大地震在主破裂前往往行成地壳变形,高应力作用下进入峰值后的变形阶段,反映到地倾斜观测日均值曲线上,表现为固体潮形变趋势异常,这些异常能否指示远距离的地震,近年来不少研究者发现,远场前兆往往出现在对应力、应变变化反应灵敏的特殊构造部位。佳木斯台可能处于这种特殊的构造部位,对东北地区浅源地震短期前兆异常“场兆”的趋势性变化具有一定的意义。     

Flow sorting of fetal erythroblasts using intracytoplasmic anti-fetal haemoglobin: Preliminary observations on maternal samples

Monoclonal antibody to fetal haemoglobin (a₂γy₂) has been proposed as a fetal-specific reagent. We developed an intracellular staining protocol that combines fluorescein isothiocyanate or phycoerythrin conjugated anti-γ with the DNA binding dye Hoechst 33342 to identify and flow sort fetal erythroblasts from maternal blood. Our preliminary observations on anti-γ-positive cells sorted from four different pregnant women are described here, using fluorescence in situ hybridization (FISH) with chromosome-specific probes to identify fetal cells. Our data demonstrate that far fewer candidate fetal cells are sorted with this protocol than by current cell surface staining methods that employ the monoclonal antibody CD71. This results in increased fetal cell sorting purities. With this protocol, standard FISH techniques require modification due to the rigorous fixation with 4 per cent paraformaldehyde. Our initial data indicate the promise of this approach.     

高强铝合金超塑挤压成形

主要介绍了工业牌号高强铝合金在供应态或经简单预处理后在通用油压机上超塑成形的实例,并分析探讨了非理想组织材料超塑成形中微观组织的变化。     

随机模拟在常州运河水质规划中的应用

以常州运河的水质规划为实例,讨论了确定性水质模型的随机模拟。讨论中引进了主观概率和客观概率的概念,用泰勒级数确定客观概率分布,用可能最大机率法估计主观概率分布,并给出了在实施不同规划方案时的水质概率曲线和水质达标的保证率。计算结果表明,使水质恶化的各种随机因素的作用不容忽视,各种水污染治理措施只能降低水质超标的风险水平。     

Atmospheric pollution in Beijing, China, as recorded in sediments of the Kunming Lake

The fluxes of heavy metals and magnetite, a fly-ash component, from coal burning, to sediments of the Kunming Lake in Beijing have increased measurably over the last fifty years. Ages were introduced to the sediment strata through Pb-210 geochronologies. Even with a doubling of coal usage by the year 2000, the anthropogenic metal fluxes will still be less than those recorded in metropolitan Tokyo and U.S. lakes.     

重庆市暴雨规律及其引发的灾害初探

暴雨是重庆市的主要灾害性天气之一，它直接或间接诱发出洪，泥石流，滑坡等山地灾害，研究暴雨规律对山地灾害预警和防灾减灾具有重要意义，本文依据重庆市1951－2000年的气象资料和灾害资料，采用谐波分析等手段分析出重庆市暴雨的年内，年际等时间分布规律和地域，城乡，迁移等空间分布规律，进而探讨了暴雨灾害特点，类型及灾害链，并提出了防治对策和措施。