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Maria Rönnqvist Thedi Ziegler Carl-Henrik von Bonsdorff Leena Maunula 《Food and environmental virology》2012,4(1):26-33
Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil
and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed
for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective
way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural
water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through
a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind
D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the
highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water
samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields
varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass
membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to
7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine
the source of AIV transmission in an outbreak situation. 相似文献
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M. Zimmer J. P. Danko S. C. Pennings A. R. Danford A. Ziegler R. F. Uglow T. H. Carefoot 《Marine Biology》2001,138(5):955-963
Three isopod species (Crustacea: Isopoda), commonly found in the intertidal and supratidal zones of the North American Pacific
coast, were studied with respect to symbiotic microbiota in their midgut glands (hepatopancreas). Ligia pallasii (Oniscidea: Ligiidae) contained high numbers of microbial symbionts in its hepatopancreatic caeca. Numbers of endosymbionts
were strongly reduced by ingestion of antibiotics. By contrast, the hepatopancreas of Idotea wosnesenskii (Valvifera: Idoteidae) and Gnorimosphaeroma oregonense (Sphaeromatidea: Sphaeromatidae) did not contain any microbiota. Results of feeding experiments suggest that microbial endosymbionts
contribute to digestive processes in L. pallasii, the most terrestrial of the three isopods that we studied. The acquisition of digestion-enhancing endosymbionts may have
been an important evolutionary step allowing isopods to colonize terrestrial habitats where relatively indigestible leaf litter
is the primary food source. By contrast, the ability to digest phenolic compounds was most developed in one of the more marine
species, suggesting that this trait may have evolved independently in isopod species that consume a phenolic-rich diet, whether
in marine habitats or on land.
Received: 28 August 2000 / Accepted: 8 December 2000 相似文献
15.
Simulating Land-Cover Change in Montane Mainland Southeast Asia 总被引:6,自引:0,他引:6
We used the conversion of land use and its effects (CLUE-s) model to simulate scenarios of land-cover change in Montane mainland
southeast Asia (MMSEA), a region in the midst of transformation due to rapid intensification of agriculture and expansion
of regional trade markets. Simulated changes affected approximately 10 % of the MMSEA landscape between 2001 and 2025 and
16 % between 2001 and 2050. Roughly 9 % of the current vegetation, which consists of native species of trees, shrubs, and
grasses, is projected to be replaced by tree plantations, tea, and other evergreen shrubs during the 50 years period. Importantly,
4 % of this transition is expected to be due to the expansion of rubber (Hevea brasiliensis), a tree plantation crop that may have important implications for local-to-regional scale hydrology because of its potentially
high water consumption in the dry season. 相似文献
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Several eco-labels for wild-caught seafood have been developed during the last decade. This article describes and analyses the criteria applied by four different eco-labelling schemes for seafood products from capture fisheries, and discusses the criteria in terms of environmental impacts, based on the ISO 14040 standard for life cycle assessment.It is concluded that the most widespread eco-label, the Marine Stewardship Council (MSC), mainly addresses the fishing stage, in particular the overexploitation of marine resources. LCA studies confirm that the fishing stage represents the most significant environmental burden, but energy consumption and emissions of anti-fouling agents at the fishing or harvesting stage contribute with significant impacts that are not being addressed by international labelling initiatives for wild-caught seafood.LCA studies show that significant environmental impacts are related to the life cycle stages after landing. This includes fish processing, transport, cooling and packaging (especially for highly processed seafood products). Hence, another challenge would be to include criteria related to the post-landing consumption of energy, certain materials and chemicals, waste handling and wastewater emissions. Minimizing product losses throughout the product chain would also be an important area for future criteria in order to avoid fishing at high environmental costs only to produce something that is later wasted.The analysis shows that the Swedish KRAV is the only one that currently addresses a range of issues that include energy and chemicals in the whole life cycle of the products. International initiatives such as MSC cover fish products from many parts of the world emphasizing ‘overexploitation of fish resources’. It is recommended, however, that international initiatives such as MSC develop criteria related to energy use and chemicals – at least at the fishing stage. Over time, other life cycle stages could be addressed as well to the extent that this is manageable. 相似文献
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