The cause and consequences of road mortality of herpetofauna in durgapur,West Bengal,India

The mortality rate of herpetofauna due to road accidents in one of the busiest tarmacked metalled roads of Durgapur, an industrial town of West Bengal, India was studied. Road survey for 27 days covering a total distance of 94.5 km was conducted. Within this span of time and the total stretch of road, 354 amphibians belonging to 6 species and 133 reptiles belonging to 11 species were observed to be killed in road-accidents. Among amphibians, Duttaphrynus melanostictus and amongst the reptiles Hemidactylus spp. were killed in highest numbers. The stretch of road under survey passed through different habitat patches like’s agricultural land, human habitation, dumping area, fragmented forest and wetland. On an average, 18 herpetofauna was noted to be killed daily. It was difficult to identify the species as the key characters were missing in the remains in a few cases of road casualties. High road mortality of herpetofauna would surely affect the balance of adjoining ecosystems as well as may be crucial for the local herpetofaunal population attributes.     

Characterization of fluoride-tolerant halophilic Bacillus flexus NM25 (HQ875778) isolated from fluoride-affected soil in Birbhum District, West Bengal, India

A new Gram-positive, nonpigmented, rod-shaped fluoride-tolerant bacterial strain, NM25, was isolated from waterlogged muddy field soil collected from the fluoride endemic area of Rampurhat II block (average fluoride in water, 4.7 mg/l, and in soil, 1.5 mg/kg) in Birbhum District, West Bengal, India. The study was undertaken to characterize the fluoride-tolerant bacterial isolate, to determine its role in bioaccumulation of fluoride, and to analyze the water and soil quality of the bacterial environment. The isolate was positive for catalase, lipase, urease, protease, oxidase, and H₂S production, but negative for indole production, nitrate reduction, and Vogues–Proskauer test. The organisms were sensitive to recommended doses of ofloxacin, kanamycin, rifampicin, levofloxacin, vancomycin, gatifloxacin, gentamicin, doxycycline, streptomycin, and nalidixic acid but resistant to ampicillin. Based on the phenotypic characteristics, 16S rRNA gene sequence, and phylogenetic analysis, the bacterial isolate NM25 was identified as Bacillus flexus. The G+C content of the 16S rDNA was 53.14 mol%. This strain tolerated up to 20 % (w/v) NaCl in nutrient agar medium and was grown at the pH range 4–12. It reduced fluoride concentration up to 67.45 % and tolerated more than 1,500 ppm of fluoride in brain–heart infusion agar medium.