22种化学物和紫外线对λDNA的遗传毒性分析

采用具有遗传毒性的受试化学物处理λDNA后,经体外包装,形成噬菌体颗粒,感染宿主菌LE392,噬菌斑发生率将显著降低。用这一方法对22种化学物和紫外线测试的结果表明,丝裂霉素C等13种受试化学物和紫外线对λDNA具有遗传毒性。根据用限制性内切酶消化和凝胶电泳分析的结果认为,丝裂霉素C（MMC）、甲基磺酸乙酯（EMS）和9－氨基吖啶（9－AA）对λDNA的遗传毒作用机理,可能是它们分别造成了λDNA分子发生随机断裂、DNA交联和在碱基热点上结合的移码突变。     

三峡库区消落带富营养化及其危害预测和防治

三峡水库蓄水运行后将出现一个落差达30 m的永久消落带。消落带是水域生态系统与岸上陆地生态系统的交替控制地带,该地带具有生物的多样性、人类活动的频繁性和生态的脆弱性,随着人类活动的影响,已成为湖岸带中生态最脆弱的地带。由于工业和生活用水污染以及水体自净能力下降,消落区富营养化相关物质（如氮和磷）污染会进一步加剧。因此,必须针对库区消落带地区的特殊情况,选择适当的模式对消落带进行超前环境整治和保护,实现三峡库区生态系统的恢复和重建。     

水浴消解－原子荧光法同时测定污水处理厂污泥中的汞和砷

本文主要介绍了应用双道原子荧光光谱法,采用水浴消解法同时测定污水处理厂污泥中汞和砷的原理和过程。结果表明,该方法具有操作简单、快速省事、灵敏度高、准确度好、节省试剂等优点,是较好的测定方法。     

Removal of antibiotics in a parallel-plate thin-film-photocatalytic reactor: Process modeling and evolution of transformation by-products and toxicity

Photocatalytic degradation of sulfamethoxazole(SMX) antibiotic has been studied under recycling batch and homogeneous flow conditions in a thin-film coated immobilized system namely parallel-plate(PPL) reactor. Experimentally designed, statistically evaluated with a factorial design(FD) approach with intent to provide a mathematical model takes into account the parameters influencing process performance. Initial antibiotic concentration, UV energy level, irradiated surface area, water matrix(ultrapure and secondary treated wastewater) and time, were defined as model parameters. A full of 2~5 experimental design was consisted of 32 random experiments. PPL reactor test experiments were carried out in order to set boundary levels for hydraulic, volumetric and defined defined process parameters. TTIP based thin-film with polyethylene glycol + TiO_2 additives were fabricated according to pre-described methodology. Antibiotic degradation was monitored by High Performance Liquid Chromatography analysis while the degradation products were specified by LC–TOF-MS analysis. Acute toxicity of untreated and treated SMX solutions was tested by standard Daphnia magna method. Based on the obtained mathematical model, the response of the immobilized PC system is described with a polynomial equation. The statistically significant positive effects are initial SMX concentration,process time and the combined effect of both, while combined effect of water matrix and irradiated surface area displays an adverse effect on the rate of antibiotic degradation by photocatalytic oxidation. Process efficiency and the validity of the acquired mathematical model was also verified for levofloxacin and cefaclor antibiotics. Immobilized PC degradation in PPL reactor configuration was found capable of providing reduced effluent toxicity by simultaneous degradation of SMX parent compound and TBPs.     

Adsorption of Disperse Blue SBL dye by synthesized poorly crystalline hydroxyapatite

The present study has been undertaken to evaluate the adsorption in batch mode of a disperse dye (Disperse Blue SBL) by poorly crystalline hydroxyapatite synthesized by coprecipitation between Ca(NO_3)_2 and (NH_4)_2HPO4 reagents in aqueous solution at room temperature. The adsorption experiments were carried out to investigate the factors that influence the dye uptake by the adsorbent, such as the contact time under agitation, adsorbent dosage, initial dye concentration, solution temperature, and pH. The e...     

城市突发性水危机及应急管理探讨

近年来.随着城市突发性水危机日益增加,如何快速、有效地应对水危机事故已成为我国亟需解决的问题.在此通过分析国内外相关政策法案及处理经验,研究城市突发性水危机的特征(包括种类和等级、相关部门和处理流程等),同时在剖析实例的基础上,探讨我国在城市水危机应急管理方面存在的问题,并提出相应的改善方案.     

4种浮床栽培植物生长特性及吸收氮磷能力的比较

选取美人蕉、风车草、菖蒲和香根草4种常见的浮床植物为研究对象,对其生长特性及吸收氮磷的能力进行比较研究.结果显示,4种植物的成活率均在90%以上,移栽后60-100d为植物的快速生长期.经过4个月的生长.4种植物水面上部生物量均明显高于下部;除香根草外,其它3种植物体内的氮磷含量均呈叶>根>茎的分布;植物体内的氮、磷累积量大小依次为美人蕉>风车草>菖蒲>香根草.植物吸收占浮床系统氮去除的比例均低于40%,占浮床系统磷去除的比例均高于55%.4种植物中,美人蕉对水体中氮磷的吸收能力最强,可以将其作为三峡库区次级河流的浮床栽培植物进行推广.     

Distribution of mercury in di erent environmental compartments in the aquatic ecosystem of the coastal zone of the Southern Baltic Sea

The aim of this study was to characterize mercury (Hg) contamination in the coastal waters of the Southern Baltic Sea, and to investigate transformations of Hg in the initial links of the marine food chain. Concentrations of Hg in water, particulate matter, plankton and macrophytes at various stations in the coastal zone (a bay with restricted water exchange, near an industrial city, river mouths, and the open sea) were measured in 2006–2008. Hg concentrations observed in the Southern Baltic varied greatly, showing the highest average values in all environmental compartments near the river mouths. In shallow, sheltered parts of the gulf, where water exchange is restricted, Hg concentrations in the water and in macrophytes were elevated relative to those in the coastal zone of the deeper part of the bay and in the open Baltic. Distance to the river mouth, terrestrial runo , and quantity and quality of organic matter were more important than seasonal variations in controlling Hg and HgSPM concentrations in water samples. Mercury concentrations in the surface microlayer at the air/sea interface were over 10 times higher than those in the bulk surface water. Concentrations of Hg in macrophytes in the winter were significantly higher than those in the warm seasons (spring, summer, autumn). This was probably the combined e ect of higher availability of Hg in porewaters and leaf growth inhibition.     

Response of soil catalase activity to chromium contamination

The impact of chromium(III) and (VI) forms on soil catalase activity was presented. The Orthic Podzol, Haplic Phaeozem and Mollic Gleysol from di erent depths were used in the experiment. The soil samples were amended with solution of Cr(III) using CrCl3, and with Cr(VI) using K2Cr2O7 in the concentration range from 0 to 20 mg/kg, whereas the samples without the addition of chromium served as control. Catalase activity was assayed by one of the commonly used spectrophotometric methods. As it was demonstrated in the experiment, both Cr(III) and Cr(VI) have an ability to reduce soil catalase activity. A chromium dosage of 20 mg/kg caused the inhibition of catalase activity and the corresponding contamination levels ranged from 75% to 92% for Cr(III) and 68% to 76% for Cr(VI), with relation to the control. Catalase activity reached maximum in the soil material from surface layers (0–25 cm), typically characterized by the highest content of organic matter creating favorable conditions for microorganisms.