Characterization of water extractable organic matter in a deep soil profile

The aim of this study was to identify qualitative and quantitative differences of water extractable organic matter (WEOM) isolated from each horizon along a deep soil profile and to evaluate any relationship between the WEOC and the total organic carbon (TOC) content. The soil profile "Monte Pietroso" is located in the Murge area, Apulia region in Southern Italy. Samples from the eight horizons (Ap1, Ap2, Ab1, Ab2, Bt1, 2B, 2Bt2, and 2B/C) were collected in October 2002. The WEOM characterization was carried out by means of UV absorbance, fluorescence spectroscopy in the emission and excitation/emission matrix (EEM) modes, and additional spectroscopic derived indexes. Soil organic carbon was shown to accumulate in the top horizons (Ap) and, in general, to decrease with depth, whereas, the WEOM/TOC ratio increases with increasing depth. The aromaticity and the humification index of the WEOM decrease dramatically downward the soil profile, whereas the fluorescence efficiency index tends to increase markedly. The WEOM fractions feature three main fluorophores with different wavelength and relative intensity. In general WEOM transport phenomena are suggested to occur downward the soil profile, depending on the nature of the organic material and on the chemical and mineral characteristics of the various horizons.     

Why do honey-bee (Apis millifera) foragers transfer nectar to several receivers? Information improvement through multiple sampling in a biological system

The task of nectar foraging in honey-bees is partitioned between foragers and receivers. Foragers typically transfer a nectar load in the nest as sub-loads to several receivers rather than as a single transfer. Foragers experience delays in finding receivers and use these delays to balance the number of foragers and receivers. A short delay results in the forager-recruiting waggle dance whereas a long delay results in the receiver-recruiting tremble dance. Several nectar transfers increase the cost of this system by introducing additional delays in finding extra receivers. We tested four hypotheses to explain the occurrence of multiple transfer. We found no evidence that multiple transfer is due to different crop capacities of foragers and receivers or that it results from extensive trophallactic interactions with nest-mates. Receiver bees frequently evaporate nectar in their mouthparts to hasten the production of honey. The suggestion has been made that multiple transfer is driven by receivers who take partial loads from foragers to enhance nectar evaporation. An alternative suggestion is that foragers drive multiple transfer to gain better information on the balance of foragers and receivers. Multiple sampling of the delay in finding a receiver reduces the standard deviation of the delay mean and so provides foragers with better information than is provided by a single delay. The enhanced-evaporation hypothesis predicts that receivers break foragers' first transfer whereas the information improvement hypothesis predicts foragers break their first transfers. Furthermore, only the information improvement hypothesis predicts a high level of multiple receptions. Data on transfer break-off and receiver behaviour strongly support the information improvement hypothesis and reject the enhanced-evaporation hypothesis. We suggest that multiple transfer is an adaptive sampling mechanism, which improves foragers' information on colony work allocation, and that multiple sampling is a common feature of social insect societies.     

Ecological boundary detection using Bayesian areal wombling

The study of ecological boundaries and their dynamics is of fundamental importance to much of ecology, biogeography, and evolution. Over the past two decades, boundary analysis (of which wombling is a subfield) has received considerable research attention, resulting in multiple approaches for the quantification of ecological boundaries. Nonetheless, few methods have been developed that can simultaneously (1) analyze spatially homogenized data sets (i.e., areal data in the form of polygons rather than point-reference data); (2) account for spatial structure in these data and uncertainty associated with them; and (3) objectively assign probabilities to boundaries once detected. Here we describe the application of a Bayesian hierarchical framework for boundary detection developed in public health, which addresses these issues but which has seen limited application in ecology. As examples, we analyze simulated spread data and the historic pattern of spread of an invasive species, the hemlock woolly adelgid (Adelges tsugae), using county-level summaries of the year of first reported infestation and several covariates potentially important to influencing the observed spread dynamics. Bayesian areal wombling is a promising approach for analyzing ecological boundaries and dynamics related to changes in the distributions of native and invasive species.     

The effect of diesel fuel on common vetch (Vicia sativa L.) plants

When petroleum hydrocarbons contaminate soil, the carbon:nitrogen (C:N) ratio of the soil is altered. The added carbon stimulates microbial numbers but causes an imbalance in the C:N ratio which may result in immobilization of soil nitrogen by the microbial biomass, leaving none available for plant growth. As members of Leguminosae fix atmospheric nitrogen to produce their own nitrogen for growth, they may prove more successful at growing on petroleum hydrocarbon contaminated sites. During a wider study on phytoremediation of diesel fuel contaminated soil, particular attention was given to the performance of legumes versus other plant species. During harvesting of pot experiments containing leguminous plants, a recurring difference in the number and formation of root nodules present on control and contaminated Common vetch (Vicia sativa L.) plants was observed. The total number of nodules per plant was significantly reduced in contaminated plants compared to control plants but nodules on contaminated plants were more developed than corresponding nodules on control plants. Plant performance of Common vetch and Westerwold's ryegrass (Lolium multiflorum L.) was compared to illustrate any difference between the ability of legumes and grasses to grow on diesel fuel contaminated soil. Common vetch was less affected by diesel fuel and performed better in low levels of diesel fuel contaminated soil than Westerwolds ryegrass. The total amount of diesel fuel remaining after 4 months in Common vetch planted soil was slightly less than in Westerwolds ryegrass planted soil.     

Mass occurrence of the ctenophore Bolinopsis vitrea (L. Agassiz, 1860) in the nearshore southern Adriatic Sea (Kotor Bay, Montenegro)

The ctenophore Bolinopsis vitrea has been rarely observed in the Mediterranean Sea. A bloom of B. vitrea is here reported for the first time in the southern Adriatic Sea in the spring and summer of 2009, together with its effect on the plankton of Kotor Bay. Ctenophores were found below 5 m depth only. Results of the investigation indicate that mass occurrence of B. vitrea could have a great impact on the Kotor Bay ecosystem. Their predation on copepods would reduce grazing pressure on phytoplankton, favouring an uncommon bloom of the latter. It is evident that B. vitrea are capable of altering rapidly the composition and biomass of coastal plankton communities when present in large masses. This first evidence of such events for this species may indicate changes in the functioning of marine ecosystems of the southern Adriatic.     

Ranked set sample design for environmental investigations

Ranked set sampling can provide an efficient basis for estimating parameters of environmental variables, particularly when sampling costs are intrinsically high. Various ranked set estimators are considered for the population mean and contrasted in terms of their efficiencies and useful- ness, with special concern for sample design considerations. Specifically, we consider the effects of the form of the underlying random variable, optimisation of efficiency and how to allocate sampling effort for best effect (e.g. one large sample or several smaller ones of the same total size). The various prospects are explored for two important positively skew random variables (lognormal and extreme value) and explicit results are given for these cases. Whilst it turns out that the best approach is to use the largest possible single sample and the optimal ranked set best linear estimator (ranked set BLUE), we find some interesting qualitatively different conclusions for the two skew distributions     

The variability of PCDD/F concentrations in the effluent of wastewater treatment plants with regard to their hydrological environment

The occurrence, concentrations, patterns, and loads of 17 2,3,7,8-substituted congeners of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) were determined in treated wastewater collected at the outlets from 14 wastewater treatment plants (WTPs), divided into three size categories. The analysis also considered the effect of wastewater outflow on the final concentrations of PCDDs/Fs, as the samples were collected during flooding and stable hydrological conditions. None of the studied WTPs were found to completely remove toxic congeners of PCDDs/Fs from wastewater: the PCDD concentrations in the outgoing effluent during stable wastewater flow ranged from 2.99 to 177.19 pg/L, PCDFs from 6.05 to 51.30 pg/L, and the Toxic Equivalent (TEQ) was between 0.94 and 4.87 pg/L. The results from high wastewater flow were less diversified, ranging from 5.04 to 8.85 pg/L for PCDDs, from 11.47 to 32.33 pg/L for PCDFs and from 2.00 to 4.65 pg/L for TEQ. The smallest WTPs demonstrated the highest average total and TEQ concentrations due to limited volume, short retention time and outdated technology, and, hence, insufficient treatment efficiency. The largest WTPs have the potential to substantially affect the quality of river water, as despite being associated with the lowest concentrations, they released much greater volumes of treated wastewater and, hence, the greatest amounts of analyzed compounds. Elevated TEQ values were observed at high flow in all WTP size categories indicating the impact of high and stable wastewater flow on the concentrations of the observed PCDDs/Fs.     

English county councils and their agenda for waste minimisation

Minimisation is the top priority of the waste management hierarchy, which is one of the guiding principals for national solid waste management planning throughout the developed world. As such it should be encouraged as a means for reducing wastes which require treatment and disposal, whether they be household, commercial or industrial in source. This paper suggests that minimisation is not being given the necessary policy frameworks or legislation within which to develop in the UK, and questions whether minimisation is being afforded the respect and attention, from all levels, that is due the most preferable waste option according to the hierarchy. This theme is investigated by surveying the county councils of England as a representative sample of waste disposal authorities in England, and former waste regulation authorities. They are responsible for guiding local and district waste policy and are the regional waste planners in the UK. If they are not seriously taking on board the message of minimisation, then there is little chance that it will succeed without further legislative developments. Some 59% of English counties have a minimisation policy, whilst only 47% have participated in and supported a minimisation programme or trial within their region. The majority of these developments have occurred within the last three years, and 78% of counties who have participated in a programme have found it a successful venture. By 1999 79% of English counties will have been actively involved in a minimisation programme in their region, which is a positive scenario. This trend must continue if waste minimisation is to become the key theme of future sustainable waste management in the UK as was intended by declarations at the Rio '92 conference and in subsequent UK Government policy and strategy.     

An Assessment of U(VI) removal from groundwater using biochar produced from hydrothermal carbonization

The ever-increasing growth of biorefineries is expected to produce huge amounts of lignocellulosic biochar as a byproduct. The hydrothermal carbonization (HTC) process to produce biochar from lignocellulosic biomass is getting more attention due to its inherent advantage of using wet biomass. In the present study, biochar was produced from switchgrass at 300 °C in subcritical water and characterized using X-ray diffraction, fourier transform infra-red spectroscopy, scanning electron micrcoscopy, and thermogravimetric analysis. The physiochemical properties indicated that biochar could serve as an excellent adsorbent to remove uranium from groundwater. A batch adsorption experiment at the natural pH (~3.9) of biochar indicated an H-type isotherm. The adsorption data was fitted using a Langmuir isotherm model and the sorption capacity was estimated to be ca. 2.12 mg of U g(-1) of biochar. The adsorption process was highly dependent on the pH of the system. An increase towards circumneutral pH resulted in the maximum adsorption of ca. 4 mg U g(-1) of biochar. The adsorption mechanism of U(VI) onto biochar was strongly related to its pH-dependent aqueous speciation. The results of the column study indicate that biochar could be used as an effective adsorbent for U(VI), as a reactive barrier medium. Overall, the biochar produced via HTC is environmentally benign, carbon neutral, and efficient in removing U(VI) from groundwater.     

Risk factors for elevated Enterococcus concentrations in a rural tropical island watershed

Associations were examined between riparian canopy cover, presence of cattle near streams, and month of year with the concentration of Enterococcus (Most Probable Number (MPN)/100 ml) in surface water at Waipā watershed on the North Side of the Hawaiian island Kaua'i. Each one percent decrease in riparian canopy cover was associated with a 3.6 MPN/100 ml increase of waterborne Enterococcus. Presence of cattle near monitoring sites was associated with an increase of 99.3 MPN/100 ml of Enterococcus in individual grab samples. Lastly, summer samples (July) were substantially higher in concentration of Enterococcus than winter collected samples (February) in Enterococcus in sampled streams. These results suggest that reducing canopy cover and introduction of cattle into riparian zones may contribute to increases of Enterococcus concentrations in stream water.