Exposure of Brassica juncea (L) to arsenic species in hydroponic medium: comparative analysis in accumulation and biochemical and transcriptional alterations

Arsenic (As) contamination in the environment has attracted considerable attention worldwide. The objective of the present study was to see the comparative effect of As species As(III) and As(V) on accumulation, biochemical responses, and gene expression analysis in Brassica juncea var. Pusa Jaganath (PJn). Hydroponically grown 14-day-old seedlings of B. juncea were treated with different concentrations of As(III) and As(V). Accumulation of total As increased with increasing concentration of both As species and exposure time, mainly in roots. Reduction in seed germination, root–shoot length, chlorophyll, and protein content were observed with increasing concentration and exposure time of both As species, being more in As(III)-treated leaves. PJn variety showed that antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)) and stress-related parameters (cysteine, proline, and malondialdehyde (MDA)) were stimulated and allows plant to tolerate both As species. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis in leaves showed significant changes in protein profile with more stringent effect with As(III) stress. Semiquantitative RT-PCR analysis showed regulation in expression of phytochelatin synthase (PCS), metallothionine-2 (MT-2), glutathione reductase (GR), and glutathione synthetase (GS) genes under both As(III) and As(V) stresses. Results suggested that accumulation and inhibition on physiological parameters differ according to the As species, while molecular and biochemical parameters showed a combinatorial type of tolerance mechanism against As(III) and As(V) stresses.     

Leachability and phytoavailability of nitrogen, phosphorus, and potassium from different bio-composts under chloride- and sulfate-dominated irrigation water

Concerns over increased phosphorus (P) application with nitrogen (N)-based compost application have shifted the trend to P-based composed application, but focusing on one or two nutritional elements does not serve the goals of sustainable agriculture. The need to understand the nutrient release and uptake from different composts has been further aggravated by the use of saline irrigation water in the recent scenario of fresh water shortage. Therefore, we evaluated the leachability and phytoavailability of P, N, and K from a sandy loam soil amended with animal, poultry, and sludge composts when applied on a total P-equivalent basis (200 kg ha(-1)) under Cl(-) (NaCl)- and SO4(2-) (Na2SO4)-dominated irrigation water. Our results showed that the concentration of dissolved reactive P (DRP) was higher in leachates under SO(4)(2-) than Cl(-) treatments. Compost amendments differed for DRP leaching in the following pattern: sludge > animal > poultry > control. Maize (Zea mays L.) growth and P uptake were severely suppressed under Cl(-) irrigation compared with SO4(2-) and non-saline treatments. All composts were applied on a total P-equivalent basis, but maximum plant (shoot + root) P uptake was observed under sludge compost amendment (73.4 mg DW(-1)), followed by poultry (39.3 mg DW(-1)), animal (15.0 mg DW(-1)), and control (1.2 mg DW(-1)) treatment. Results of this study reveal that irrigation water dominated by SO4(2-) has greater ability to replace/leach P, other anions (NO3(-)), and cations (K+). Variability in P release from different bio-composts applied on a total P-equivalent basis suggested that P availability is highly dependent on compost source.     

Clear effects of soil organic matter chemistry, as determined by NMR spectroscopy, on the sorption of diuron

Organic matter has long been recognized as the main sorbent phase in soils for hydrophobic organic compounds (HOCs). In recent times, there has been an increasing realization that not only the amount, but also the chemical composition, of organic matter can influence the sorption properties of a soil. Here, we show that the organic carbon-normalized sorption coefficient (K(OC)) for diuron is 27-81% higher in 10 A11 horizons than in 10 matching A12 horizons for soils collected from a small (2ha) field. K(OC) was generally greater for the deeper (B) horizons, although these values may be inflated by sorption of diuron to clays. Organic matter chemistry of the A11 and A12 horizons was determined using solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. K(OC) was positively correlated with aryl C (r2=0.59, significance level 0.001) and negatively correlated with O-alkyl C (r2=0.84, significance level <0.001). This is only the second report of correlations between whole soil K(OC) and NMR-derived measures of organic matter chemistry. We suggest that this success may be a consequence of limiting this study to a very small area (a single field). There is growing evidence that interactions between organic matter and clay minerals strongly affect K(OC). However, because the soil mineralogy varies little across the field, the influence of these interactions is greatly diminished, allowing the effect of organic matter chemistry on K(OC) to be seen clearly. This study in some way reconciles studies that show strong correlations between K(OC) and the chemistry of purified organic materials and the general lack of such correlations for whole soils.     

Separating the effects of organic matter-mineral interactions and organic matter chemistry on the sorption of diuron and phenanthrene

Even though it is well established that soil C content is the primary determinant of the sorption affinity of soils for non-ionic compounds, it is also clear that organic carbon-normalized sorption coefficients (K(OC)) vary considerably between soils. Two factors that may contribute to K(OC) variability are variations in organic matter chemistry between soils and interactions between organic matter and soil minerals. Here, we quantify these effects for two non-ionic sorbates-diuron and phenanthrene. The effect of organic matter-mineral interactions were evaluated by comparing K(OC) for demineralized (HF-treated) soils, with K(OC) for the corresponding whole soils. For diuron and phenanthrene, average ratios of K(OC) of the HF-treated soils to K(OC) of the whole soils were 2.5 and 2.3, respectively, indicating a substantial depression of K(OC) due to the presence of minerals in the whole soils. The effect of organic matter chemistry was determined by correlating K(OC) against distributions of C types determined using solid-state (13)C NMR spectroscopy. For diuron, K(OC) was positively correlated with aryl C and negatively correlated with O-alkyl C, for both whole and HF-treated soils, whereas for phenanthrene, these correlations were only present for the HF-treated soils. We suggest that the lack of a clear effect of organic matter chemistry on whole soil K(OC) for phenanthrene is due to an over-riding influence of organic matter-mineral interactions in this case. This hypothesis is supported by a correlation between the increase in K(OC) on HF-treatment and the soil clay content for phenanthrene, but not for diuron.     

28-homobrassinolide protects chickpea (Cicer arietinum) from cadmium toxicity by stimulating antioxidants

In the present experiment the seeds of Cicer arietinum (L.) cv. Uday were inoculated with specific Rhizobium grown in sandy loam soil and were allowed to grow for 15 days. At this stage, the seedlings were supplied with 0, 50, 100 or 150 microM of cadmium in the form of cadmium chloride and sprayed with 0.01 microM of 28-homobrassinolide (HBL) at 30-day stage. The data indicated that plant fresh and dry mass, number of nodules, their fresh and dry mass, leghemoglobin content, nitrogen and carbohydrate content in the nodules, leaf chlorophyll content, nitrate reductase and carbonic anhydrase activities decreased proportionately with the increasing concentrations of cadmium but the content of proline and the activities of catalase, peroxidase and superoxide dismutase increased. The ill effect, generated by cadmium, was overcome if the stressed plants were sprayed with HBL.     

Municipal solid waste management in Indian cities - A review

Municipal solid waste management (MSWM) is one of the major environmental problems of Indian cities. Improper management of municipal solid waste (MSW) causes hazards to inhabitants. Various studies reveal that about 90% of MSW is disposed of unscientifically in open dumps and landfills, creating problems to public health and the environment. In the present study, an attempt has been made to provide a comprehensive review of the characteristics, generation, collection and transportation, disposal and treatment technologies of MSW practiced in India. The study pertaining to MSWM for Indian cities has been carried out to evaluate the current status and identify the major problems. Various adopted treatment technologies for MSW are critically reviewed, along with their advantages and limitations. The study is concluded with a few fruitful suggestions, which may be beneficial to encourage the competent authorities/researchers to work towards further improvement of the present system.     

Optimal redesign of groundwater quality monitoring networks: a case study

Assessment and redesign of water quality monitoring networks is an important task in water quality management. This paper presents a new methodology for optimal redesign of groundwater quality monitoring networks. The measure of transinformation in discrete entropy theory and the transinformation–distance (T–D) curves are used to quantify the efficiency of sampling locations and sampling frequencies in a monitoring network. The existing uncertainties in the T–D curves are taken in to account using the fuzzy set theory. The C-means clustering method is also used to classify the study area to some homogenous zones. The fuzzy T–D curve of the zones is then used in a multi-objective hybrid genetic algorithm-based optimization model. The proposed methodology is utilized for optimal redesign of monitoring network of the Tehran aquifer in the Tehran metropolitan area, Iran.     

Measurement of soil radioactivity levels and radiation hazard assessment in southern Rechna interfluvial region, Pakistan

Rechna interfluvial region is one of the main regions of Punjab, Pakistan. It is the area which is lying between River Ravi and River Chenab, alluvial-filled. Radioactivity levels in soil samples, collected from southern Rechna interfluvial region, Pakistan, have been estimated by using gamma-ray spectrometric technique. ²²⁶Ra, ²³²Th, the primordial radionuclide ⁴⁰K, and the artificial radionuclide ¹³⁷Cs have been measured in the soil of the study area. The mean radioactivity levels of ²²⁶Ra, ²³²Th, ⁴⁰K, and ¹³⁷Cs were found to be 50.6 ± 1.7, 62.3 ± 3.2, 662.2 ± 32.1, and 3.1 ± 0.3 Bq kg^???1, respectively. The mean radium equivalent activity (Ra_eq), outdoor radiation hazard index (H _out), indoor radiation hazard index (H _in), and terrestrial absorbed dose rate for the area under study were determined as 190.8 ± 8.7 Bq kg^???1, 0.52, 0.65, and 69.8 nGy h^???1, respectively. The annual effective dose to the general public was found to be 0.43 mSv. This value lies well below the limit of 1 mSv for general public as recommended by the International Commission on Radiological Protection. The measured values are comparable with other global radioactivity measurements and are found to be safe for the public and the environment.     

Fuzzy Possibilistic Modeling for Closed Loop Recycling Collection Networks

Nowadays, in the competitive market, commercial companies due to their economic problems and also restrictions imposed by international organizations to comply with environmental regulations are making noticeable efforts to reduce the level of their wastes in their manufacturing systems and consequently the level of waste in consumers’ products. In harmony with this issue, one of the most effective ways which has successfully been used and proven to be economically profitable is to take products lifecycles into consideration. Consideration of products lifecycle has made supply chain practitioners to investigate the reverse logistics activities in addition to forward logistics activities. Hence, corporations, in order to reduce their cost on the one hand and boosting their efficiency on the other hand, were obligated to employ closed loop supply chain models to concurrently benefit from its economical and environmental advantages. Therefore, in this paper, a mixed nonlinear facility location–allocation model is proposed for recycling collection centers. The considered closed loop logistics model consists of multiple echelons, multiple suppliers, multiple collection centers, multiple time period and also multiple facilities. In real-life problems parameters like demand, cost, capacity, distances, and quantities of returned products are always uncertain. Therefore, in order to solve a realistic problem, foregoing parameters are considered as fuzzy in our proposed model. Subsequently, to solve fuzzy mixed nonlinear programming model, one of the most effective technique in the literature is used. Additionally, in order to demonstrate the behavior of the parameters employed in the model, a comprehensive sensitivity analysis is conducted. Computational results demonstrate that the proposed model can show favorable efficiency in solving supply chain problems.     

Mercury contamination in the estuaries and coastal sediments of the Strait of Malacca

Fishers’ local ecological knowledge (LEK) is an additional tool to obtain information about cetaceans, regarding their local particularities, fishing interactions, and behavior. However, this knowledge could vary in depth of detail according to the level of interaction that fishers have with a specific species. This study investigated differences in small-scale fishers’ LEK regarding the estuarine dolphin (Sotalia guianensis) in three Brazilian northeast coastal communities where fishing is practiced in estuarine lagoons and/or coastal waters and where dolphin-watching tourism varies from incipient to important. The fishers (N?=?116) were asked about general characteristics of S. guianensis and their interactions with this dolphin during fishing activities. Compared to lagoon fishers, coastal fishers showed greater knowledge about the species but had more negative interactions with the dolphin during fishing activities. Coastal fishing not only offered the opportunity for fishers to observe a wider variety of the dolphin’s behavior, but also implied direct contact with the dolphins, as they are bycaught in coastal gillnets. Besides complementing information that could be used for the management of cetaceans, this study shows that the type of environment most used by fishers also affects the accuracy of the information they provide. When designing studies to gather information on species and/or populations with the support of fishers, special consideration should be given to local particularities such as gear and habitats used within the fishing community.