A Heuristic Method for Determining Changes of Source Loads to Comply with Water Quality Limits in Catchments

A common land and water management task is to determine where and by how much source loadings need to change to meet water quality limits in receiving environments. This paper addresses the problem of quantifying changes in loading when limits are specified in many locations in a large and spatially heterogeneous catchment, accounting for cumulative downstream impacts. Current approaches to this problem tend to use either scenario analysis or optimization, which suffer from difficulties of generating scenarios that meet the limits, or high complexity of optimization approaches. In contrast, we present a novel method in which simple catchment models, load limits, upstream/downstream spatial relationships and spatial allocation rules are combined to arrive at source load changes. The process iteratively establishes the critical location (river segment or lake) where the limits are most constraining, and then adjusts sources upstream of the critical location to meet the limit at that location. The method is demonstrated with application to New Zealand (268,000 km2) for nutrients and the microbial indicator E. coli, which was conducted to support policy development regarding water quality limits. The model provided useful insights, such as a source load excess (the need for source load reduction) even after mitigation measures are introduced in order to comply with E. coli limits. On the other hand, there was headroom (ability to increase source loading) for nutrients. The method enables assessment of the necessary source load reductions to achieve water quality limits over broad areas such as large catchments or whole regions.     

Evolutionary variation in the expression of phenotypically plastic color vision in Caribbean mantis shrimps, genus Neogonodactylus

Many animals have color vision systems that are well suited to their local environments. Changes in color vision can occur over long periods (evolutionary time), or over relatively short periods such as during development. A select few animals, including stomatopod crustaceans, are able to adjust their systems of color vision directly in response to varying environmental stimuli. Recently, it has been shown that juveniles of some stomatopod species that inhabit a range of depths can spectrally tune their color vision to local light conditions through spectral changes in filters contained in specialized photoreceptors. The present study quantifies the potential for spectral tuning in adults of three species of Caribbean Neogonodactylus stomatopods that differ in their depth ranges to assess how ecology and evolutionary history influence the expression of phenotypically plastic color vision in adult stomatopods. After 12 weeks in either a full-spectrum “white” or a narrow-spectrum “blue” light treatment, each of the three species evidenced distinctive tuning abilities with respect to the light environment that could be related to its natural depth range. A molecular phylogeny generated using mitochondrial cytochrome oxidase C subunit 1 (CO-1) was used to determine whether tuning abilities were phylogenetically or ecologically constrained. Although the sister taxa Neogonodactylus wennerae and N. bredini both exhibited spectral tuning, their ecology (i.e. preferred depth range) strongly influenced the expression of the phenotypically plastic color vision trait. Our results indicate that adult stomatopods have evolved the ability to undergo habitat-specific spectral tuning, allowing rapid facultative physiological modification to suit ecological constraints.     

Governing trade-offs in ecosystem services and disservices to achieve human–wildlife coexistence

Sustaining wildlife populations, which provide both ecosystem services and disservices, represents a worldwide conservation challenge. The ecosystem services and Ostrom's social–ecological systems frameworks have been adopted across natural and social sciences to characterize benefits from nature. Despite their generalizability, individually they do not include explicit tools for addressing the sustainable management of many wildlife populations. For instance, Ostrom's framework does not specifically address competing perspectives on wildlife, whereas the ecosystem services framework provides a limited representation of the social and governance context wherein such competing perspectives are embedded. We developed a unified social–ecological framework of ecosystem disservices and services (SEEDS) that advances both frameworks by explicitly acknowledging the importance of competing wildlife perspectives embedded in the social and governance contexts. The SEEDS framework emulates the hierarchical structure of Ostrom's social–ecological systems, but adds subsystems reflecting heterogeneous stakeholder views and experiences of wildlife-based services and disservices. To facilitate operationalizing SEEDS and further broader analyses across human–wildlife systems, we devised a list of variables to describe SEEDS subsystems, such as types and level of services and disservices, cost and benefit sharing, and social participation of stakeholders. Steps to implement SEEDS involve engaging local communities and stakeholders to define the subsystems, analyze interactions and outcomes, and identify leverage points and actions to remedy unwanted outcomes. These steps connect SEEDS with other existing approaches in social–ecological research and can guide analyses across systems or within individual systems to provide new insights and management options for sustainable human–wildlife coexistence.     

Measurements of Cd, Cu, Pb and Zn in the lower reaches of major Eurasian arctic rivers using trace metal clean techniques

Concentrations of dissolved and particulate Cd, Cu, Pb and Zn were determined in samples collected in summer 1998 from the lower reaches of six major Eurasian arctic rivers: the Onega, Severnaya Dvina, Mezen, Pechora, Ob and Yenisey. These data comprise some of the earliest measurements of trace metals in Eurasian arctic rivers above the estuaries using recognized clean techniques. Significant (α = 0.05) differences were observed among mean concentrations of particulate metals in the individual rivers (F ≤ 0.006), with highest levels overall observed in the Severnaya Dvina and Yenisey. No significant differences were observed among mean concentrations of dissolved metals in the individual rivers (F = 0.10-0.84). Contributions from anthropogenic sources are suggested by comparison of trace metal ratios in the samples to crustal abundances. These results establish a baseline for assessing future responses of Eurasian arctic river systems to climate-related environmental changes and shifting patterns of pollutant discharge.     

Relationships between stable isotopes and metal contaminants in feathers are spurious and biologically uninformative

Stable isotopes of carbon (δ¹³C) and nitrogen (δ¹⁵N) are used frequently in ecotoxicological investigations of birds to relate contaminant levels to trophic position (δ¹⁵N) or foraging location (δ¹³C) and many researchers using avian feathers in such investigations use δ¹³C or δ¹⁵N as a predictor of contaminant concentrations. Contaminants, especially mercury, however, are integrated into feathers over different time periods than are stable isotopes, resulting in spurious relationships that have no biological meaning. I show the fundamental principles behind the conclusion that relating δ¹³C or δ¹⁵N to contaminant concentrations in feathers is not appropriate in light of the number of recent studies that have employed this approach, and make recommendations for those wishing to investigate the relationship between contaminants and stable isotope ratios.     

Estimated validity and reliability of on-board diagnostics for older vehicles: comparison with remote sensing observations

Based on requirements under the Clean Air Act Amendments of 1990, most state vehicle inspection and maintenance (I/M) programs have, since 2002, replaced the tailpipe emission testing with the on-board diagnostic (OBD) II testing for 1996 model and newer vehicles. This test relies on the OBD II system to give the pass or fail result, depending on certain conditions that might cause the vehicle to emit pollution 1.5 times higher than the regulated standard. The OBD II system is a computer and sensors installed in the vehicle to monitor the emission control units and signal if there is any malfunction. As a vehicle ages, its engine, pollution control units, and OBD II system deteriorate. Because the OBD II system's durability directly influences the test outcome, it is important to examine the fleetwide trend in the OBD II test results in comparison with an alternative measure of identifying high emitting vehicles. This study investigates whether the validity and reliability of the OBD II test is related to the age of the OBD II system installed in the fleet. Using Atlanta's I/M testing records and remote sensing device (RSD) data collected during 2002-2005, this research establishes the convergent validity and interobserver reliability criteria for the OBD II test based on on-road emissions measured by RSDs. The study results show that older vehicles exhibit significantly lower RSD-OBD II outcome agreement than newer vehicles. This suggests that the validity and reliability of the OBD II test may decline in the older vehicle fleets. Explanations and possible confounding factors for these findings are discussed.     

The effect of deoxynivalenol on the secretion activity, proliferation and apoptosis of porcine ovarian granulosa cells in vitro

The aim of this in vitro study was to examine the secretion activity, markers of proliferation and apoptosis in porcine ovarian granulosa cells (GCs) after deoxynivalenol (DON) addition. Ovarian granulosa cells were incubated with DON for 24h: 10, 100 and 1000 ng/mL, while the control group received no DON. The secretion of insulin-like growth factor I (IGF-I) and progesterone was determined by radioimmunoassay (RIA) and expression of cyclin B1, PCNA and caspase-3 by immunocytochemistry. IGF-I release by GCs was inhibited by DON, while progesterone release and the expression of cyclin B1 was stimulated by DON (at 1000 ng/mL but not at 10 and 100 ng/mL). PCNA expression was stimulated by DON (at 100 and 1000 ng/mL but not at 10 ng/mL). Caspase-3 expression was not influenced by DON treatment (at all doses). In conclusion, our results indicate, (1) a direct effect of DON on secretion of growth factor IGF-I and steroid hormone progesterone, (2) expression of markers of proliferation (cyclin B1 and PCNA) but not on the (3) expression of marker of apoptosis (caspase-3) in porcine ovarian granulosa cells. This in vitro study suggests the dose-dependent association of DON on porcine ovarian functions.     

A new approach to data evaluation in the non-target screening of organic trace substances in water analysis

Non-target screening via high performance liquid chromatography-mass spectrometry (HPLC-MS) has gained increasingly in importance for monitoring organic trace substances in water resources targeted for the production of drinking water. In this article a new approach for evaluating the data from non-target HPLC-MS screening in water is introduced and its advantages are demonstrated using the supply of drinking water as an example. The crucial difference between this and other approaches is the comparison of samples based on compounds (features) determined by their full scan data. In so doing, we take advantage of the temporal, spatial, or process-based relationships among the samples by applying the set operators, UNION, INTERSECT, and COMPLEMENT to the features of each sample. This approach regards all compounds, detectable by the used analytical method. That is the fundamental meaning of non-target screening, which includes all analytical information from the applied technique for further data evaluation. In the given example, in just one step, all detected features (1729) of a landfill leachate sample could be examined for their relevant influences on water purification respectively drinking water. This study shows that 1721 out of 1729 features were not relevant for the water purification. Only eight features could be determined in the untreated water and three of them were found in the final drinking water after ozonation. In so doing, it was possible to identify 1-adamantylamine as contamination of the landfill in the drinking water at a concentration in the range of 20 ng L⁻¹. To support the identification of relevant compounds and their transformation products, the DAIOS database (Database-Assisted Identification of Organic Substances) was used. This database concept includes some functions such as product ion search to increase the efficiency of the database query after the screening. To identify related transformation products the database function “transformation tree” was used.     

Uncertainty in Integrated Assessment Models of Climate Change: Alternative Analytical Approaches

Uncertainty plays a key role in the economics of climate change, and research on this topic has led to a substantial body of literature. However, the discussion on the policy implications of uncertainty is still far from being settled, partly because the uncertainty of climate change comes from a variety of sources and takes diverse forms. To reflect the multifaceted nature of climate change uncertainty better, an increasing number of analytical approaches have been used in the studies of integrated assessment models of climate change. The employed approaches could be seen as complements rather than as substitutes, each of which possesses distinctive strength for addressing a particular type of problems. We review these approaches—specifically, the non-recursive stochastic programming, the real option analysis, and the stochastic dynamic programming—their corresponding literatures and their respective policy implications. We also identify the current research gaps associated with the need for further developments of new analytical approaches.     

Congenital hereditary hypothyroidism—prenatal diagnosis and treatment

Intrauterine diagnosis of congenital hypothyroidism was established on the basis of TSH concentration in amniotic fluid in the 22nd week of gestation for the offspring of a couple both known to have an iodide organification defect. Prenatal treatment consisted of intramniotic injections of 500 meg Na-1-thyroxine, which was administered from the first amniocentesis until one week before delivery. Following delivery, the diagnosis was confirmed by the elevated level of TSH, 60-5 uU/ml, and a gradual decrease of fT4 to 0·8 ng/ml. Regular substitution therapy was commenced on the third day of life. The normal shape and location of the thyroid gland was demonstrated by Technetium scintiscan. At 18 months the infant revealed no significant deviation from normalcy in growth or mental capacity. This experience indicates that testing of amniotic fluid for TSH in the 22nd week of gestation can be diagnostic for congenital primary hypothyroidism. Furthermore, it is suggested that the treatment approach described is warranted in all cases in which there is a high risk of congenital primary hypothyroidism.