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Leão Marcos Lorran Paranhos Penteado Julia Oliveira Ulguim Sabrina Morales Gabriel Rômulo Reginato dos Santos Marina Brum Aline Neutzling Zhang Linjie da Silva Júnior Flavio Manoel Rodrigues 《Environmental science and pollution research international》2021,28(31):41843-41850
Environmental Science and Pollution Research - Studies around the world have revealed reduced levels of atmospheric particulate matter in periods of greatest human mobility restriction to contain... 相似文献
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Prevalence of Human Noroviruses in Frozen Marketed Shellfish,Red Fruits and Fresh Vegetables 总被引:1,自引:0,他引:1
Julie Loutreul Catherine Cazeaux Delphine Levert Aline Nicolas Sandrine Vautier Anne Laure Le Sauvage Sylvie Perelle Thierry Morin 《Food and environmental virology》2014,6(3):157-168
Noroviruses (NoVs), currently recognised as the most common human food-borne pathogens, are ubiquitous in the environment and can be transmitted to humans through multiple foodstuffs. In this study, we evaluated the prevalence of human NoV genogroups I (GI) and II (GII) in 493 food samples including soft red fruits (n = 200), salad vegetables (n = 210) and bivalve mollusc shellfish (n = 83), using the Bovine Enterovirus type 1 as process extraction control for the first time. Viral extractions were performed by elution concentration and genome detection by TaqMan Real-Time RT-PCR (RT-qPCR). Experimental contamination using hepatitis A virus (HAV) was used to determine the limit of detection (LOD) of the extraction methods. Positive detections were obtained from 2 g of digestive tissues of oysters or mussels kept for 16 h in seawater containing 2.0–2.7 log10 plaque-forming units (PFU)/L of HAV. For lettuces and raspberries, the LOD was, respectively, estimated at 2.2 and 2.9 log10 PFU per 25 g. Of the molluscs tested, 8.4 and 14.4 % were, respectively, positive for the presence of GI NoV and GII NoV RNA. Prevalence in GI NoVs varied from 11.9 % for the salad vegetables samples to 15.5 % for the red soft fruits. Only 0.5 % of the salad and red soft fruits samples were positive for GII NoVs. These results highlight the high occurrence of human NoVs in foodstuffs that can be eaten raw or after a moderate technological processing or treatment. The determination of the risk of infection associated with an RT-qPCR positive sample remains an important challenge for the future. 相似文献
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Dória Aline R. Santos Géssica O. S. Pelegrinelli Mariane M. S. Silva Deyvid C. de Matos Danielle B. Cavalcanti Eliane Bezerra Silva Ronaldo S. Salazar-Banda Giancarlo R. Eguiluz Katlin I. B. 《Environmental science and pollution research international》2021,28(19):23634-23646
Environmental Science and Pollution Research - In this study, binary and ternary mixed metal oxide anodes of Ti/RuO2–Sb2O4 and Ti/RuO2–Sb2O4–TiO2 were prepared using two different... 相似文献
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Janine Martinazzo Daniela K. Muenchen Alexandra N. Brezolin Alana M. Cezaro Aline A. Rigo Alexandra Manzoli 《Journal of environmental science and health. Part. B》2018,53(4):229-236
The aim of this study was to develop a cantilever nanobiosensor for atrazine detection in liquid medium by immobilising the biological recognition element (tyrosinase vegetal extract) on its surface with self-assembled monolayers using gold, 16-mercaptohexadecanoic acid, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/n-hydroxysuccinimide. Cantilever nanobiosensors presented a surface compression tension increase when atrazine concentrations were increased, with a limit of detection and limit of quantification of 7.754 ppb (parts per billion) and 22.792 ppb, respectively. From the voltage results obtained, the evaluation of atrazine contamination in river and drinking water were very close to those of the reference sample and ultrapure water, demonstrating the ability of the cantilever nanobiosensor to distinguish different water samples and different concentrations of atrazine. Cantilever nanosensor surface functionalization was characterised by combining polarisation modulation infrared reflection-absorption spectroscopy and atomic force microscopy and indicating film thickness in nanometric scale (80.2 ± 0.4 nm). Thus, the cantilever nanobiosensor developed for this study using low cost tyrosinase vegetal extract was adequate for atrazine detection, a potential tool in the environmental field. 相似文献
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Góes Mariana Moraes Merci Aline Andrello Avacir Casanova Yamashita Fabio de Carvalho Gizilene Maria 《Journal of Polymers and the Environment》2021,29(6):2000-2012
Journal of Polymers and the Environment - The search for highly productive agriculture has considerably increased the use of fertilizers, becoming a worrying source of environmental and health... 相似文献
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Aline Andressa Rigo Alana Marie De Cezaro Daniela Kunkel Muenchen Janine Martinazzo Alexandra Manzoli Juliana Steffens 《Journal of environmental science and health. Part. B》2020,55(3):239-249
AbstractHeavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80?nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water. 相似文献
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Mendes Bruna de Oliveira Rabelo Letícia Martins e Silva Bianca Costa de Souza Joyce Moreira da Silva Castro André Luis da Silva Anderson Rodrigo de Lima Rodrigues Aline Sueli Malafaia Guilherme 《Environmental science and pollution research international》2017,24(23):19234-19248
Environmental Science and Pollution Research - The current study has assessed whether the oral and/or dermal exposure of C57Bl/6 J mice to tannery effluent (a complex pollutant consisting of... 相似文献
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Camila T. Marques Simone M. Golunski Bruno Venturin Aline F. Camargo Thamarys Scapini Daiane Baldissarelli Tatiani A. Modkovski Karina Paula Preczeski Camila Dalla Rosa Gean D. L. P. Vargas Jaqueline Garda Buffon Catiusa K. Rosin Alexandre Arenzon Altemir J. Mossi Helen Treichel 《环境质量管理》2017,27(2):25-31
The present study investigates the application of a noncommercial peroxidase enzyme, which was extracted from rice bran, to decolorize wastewater that was simulating effluent from a textile manufacturer using a red dye in its coloration process. 相似文献
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José Cavalcante Souza Vieira Camila Pereira Braga Grasieli de Oliveira Aline de Lima Leite João Vitor de Queiroz Bruna Cavecci Alis Correia Bittarello Marília Afonso Rabelo Buzalaf Luiz Fabricio Zara Pedro de Magalhães Padilha 《Environmental Chemistry Letters》2017,15(4):717-724
Bioaccumulative metals such as mercury are found in increasing amounts in fish and their consumers. In the region of the Madeira River, in the Brazilian Amazon, mercury (Hg) is a predominant contaminant in the aquatic ecosystem. There is therefore a need to find specific biomarkers of mercury toxicity in fish to monitor contaminations. Here, mercury-bound proteins were identified in the liver tissues of fishes Mylossoma duriventre and Brachyplatystoma rousseauxii. Mercury was quantified in liver tissue, pellets and protein spots by graphite furnace atomic absorption. Proteins were fractionated by two-dimensional polyacrylamide gel electrophoresis and identified by mass spectrometry with electrospray ionization. We identified nine proteins linked to mercury and that presented biomarker characteristics of mercury. Among the proteins identified, isoforms of parvalbumin, ubiquitin-40S ribosomal protein S27a, brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 and betaine–homocysteine S-methyltransferase 1 are notable for having the molecular function of binding to metallic ions. 相似文献