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31.
A sandwich enzyme-linked immunosorbant assay (ELISA) was developed to quantitatively detect Bullfrog (Rana catesbeiana) vitellogenin (VTG) levels. This procedure involved a sandwich ELISA using monoclonal antibodies of BVmA1 and BVmA2 against Bullfrog-VTG, and BVmA1 conjugated to horseradish peroxidase as the detection antibody. The assay range was between 9.4 ng/ml and 1200 ng/ml and the recovery of the VTG added to Bullfrog control male serum was 92.0-108.8%. Male Bullfrog was induced by injection 17beta-estradiol (E2) for four weeks and Bullfrog-VTG levels were measured each week. Histological analysis was performed for investigating the correlation of the effect to male reproduction and Bullfrog-VTG level variation depending on E2 dose. After two weeks of E2 exposure, the induced Bullfrog-VTG level was significantly higher than Bullfrog control female (p<0.05). After four weeks of E2 exposure, the rupture and fusion of seminiferous tubule in the testes of male Bullfrog were shown and provided direct evidence that the reproduction of male Bullfrog was affected by estrogenic compounds. Bullfrog-VTG bioassay, using the sandwich ELISA, could be a sensitive and useful tool for quantification of estrogenic principles in aquatic environments. 相似文献
32.
Park K Chow JC Watson JG Trimble DL Doraiswamy P Park K Arnott WP Stroud KR Bowers K Bode R Petzold A Hansen AD 《Journal of the Air & Waste Management Association (1995)》2006,56(4):474-491
Results from six continuous and semicontinuous black carbon (BC) and elemental carbon (EC) measurement methods are compared for ambient samples collected from December 2003 through November 2004 at the Fresno Supersite in California. Instruments included a multi-angle absorption photometer (MAAP; lambda = 670 nm); a dual-wavelength (lambda = 370 and 880 nm) aethalometer; seven-color (lambda = 370, 470, 520, 590, 660, 880, and 950 nm) aethalometers; the Sunset Laboratory carbon aerosol analysis field instrument; a photoacoustic light absorption analyzer (lambda = 1047 nm); and the R&P 5400 ambient carbon particulate monitor. All of these acquired BC or EC measurements over periods of 1 min to 1 hr. Twenty-four-hour integrated filter samples were also acquired and analyzed by the Interagency Monitoring of Protected Visual Environments (IMPROVE) thermal/optical reflectance carbon analysis protocol. Site-specific mass absorption efficiencies estimated by comparing light absorption with IMPROVE EC concentrations were 5.5 m2/g for the MAAP, 10 m2/g for the aethalometer at a wavelength of 880 nm, and 2.3 m2/g for the photoacoustic analyzer; these differed from the default efficiencies of 6.5, 16.6, and 5 m2/g, respectively. Scaling absorption by inverse wavelength did not provide equivalent light absorption coefficients among the instruments for the Fresno aerosol measurements. Ratios of light absorption at 370 nm to those at 880 nm from the aethalometer were nearly twice as high in winter as in summer. This is consistent with wintertime contributions from vehicle exhaust and from residential wood combustion, which is believed to absorb more shorter-wavelength light. To reconcile BC and EC measurements obtained by different methods, a better understanding is needed of the wavelength dependence of light-absorption and mass-absorption efficiencies and how they vary with different aerosol composition. 相似文献
33.
Shamil J. Cathum Amie Boudreau Alison Obenauf Andre Dumouchel Carl E. Brown Monique Punt 《补救:环境净化治理成本、技术与工艺杂志》2006,16(4):43-56
This study investigated the effectiveness of a cyclodextrin‐based solid material for the removal of mixed dissolved contaminants. The solid material was prepared by condensation of α‐cyclodextrin. The removal efficiency was found to be 70 percent for total heavy metals (cadmium, lead, chromium, iron, nickel, cobalt, and mercury) to 98 percent for polychlorinated biphenyls (PCBs). The optimum pH for heavy metal removal was approximately 5 and for PCBs it was in the range of 5–7. All of these heavy metals were successfully recovered from the spent cyclodextrin‐based material using nitric acid, allowing the material to be reused for further passes. The results also showed that the presence of alkaline and alkaline earth metals did not have a significant effect on the removal efficiency, indicating that the cyclodextrin‐based material could selectively remove the heavy metals of concern without being consumed by alkaline and alkaline‐earth metals. © 2006 Government of Canada. 相似文献
34.
For counting viruses, the plaque method and the M.P.N. method with at least 40 inoculum are both used with the same success. The precision of the results is quite equivalent for the two methods with the same volume inoculated whatever the total surface of KB cells. 相似文献
35.
A 'livelihoods gap' has become evident in international aid delivery to Somalia. Existing aid interventions do not address the economic dynamics of vulnerability resulting from Somalia's long history of predatory development and asset stripping. To prevent poor households' regular return to sub-subsistence income levels after a brief period of plenty, this paper argues that aid agencies should reorient and expand existing interventions to assist poor households to capitalise on temporary improvements in environmental and security conditions. As a corollary to emergency relief and efforts to construct state institutions, it is necessary to devise country-wide interventions that will rebuild household asset bases by protecting savings during times of stress and ensuring that markets benefit poor producers. 相似文献
36.
Testolin RC Tischer V Lima AO Cotelle S Férard JF Radetski CM 《Environmental science and pollution research international》2012,19(6):2186-2194
Background, aim and scope
Agrochemicals could reach aquatic ecosystems and damage ecosystem functionality. Natural formicide could be an alternative to use in comparison with the more toxic formicides available on the market. Thus, the objective of this study was to assess the ecotoxicity of the new natural formicide Macex? with a battery of classical aquatic ecotoxicity tests.Material and methods
Bacteria (Aliivibrio fischeri), algae (Pseudokirchneriella subcapitata), hydra (Hydra attenuata), daphnids (Daphnia magna), and fish (Danio rerio) tests were performed in accordance with international standardized methodologies.Results
In the range of formicide concentrations tested (0.03 to 2.0?g?L?1) EC50 values varied from 0.49 to >2.0?g?L?1, with P. subcapitata being the most sensitive species and H. attenuata and D. rerio the most tolerant species to this product in aqueous solutions.Conclusions
This new formicide preparation can be classed as a product of low toxicity compared to the aquatic ecotoxicity of the most common commercialized formicides. 相似文献37.
Katarzyna A. Michalow Dorota Flak Andre Heel Magdalena Parlinska-Wojtan Mieczyslaw Rekas Thomas Graule 《Environmental science and pollution research international》2012,19(9):3696-3708
TiO2:Nb nanopowders within a dopant concentration in the range of 0.1?C15?at.% were prepared by one-step flame spray synthesis. Effect of niobium doping on structural, optical and photocatalytic properties of titanium dioxide nanopowders was studied. Morphology and structure were investigated by means of Brunauer?CEmmett?CTeller isotherm, X-ray diffraction and transmission electron microscopy. Diffuse reflectance and the resulting band gap energy were determined by diffuse reflectance spectroscopy. Photocatalytic activity of the investigated nanopowders was revised for the photodecomposition of methylene blue (MB), methyl orange (MO) and 4-chlorophenol under UVA and VIS light irradiation. Commercial TiO2-P25 nanopowder was used as a reference. The specific surface area of the powders was ranging from 42.9?m2/g for TiO2:0.1?at.% Nb to 90.0?m2/g for TiO2:15?at.% Nb. TiO2:Nb particles were nanosized, spherically shaped and polycrystalline. Anatase was the predominant phase in all samples. The anatase-related transition was at 3.31?eV and rutile-related one at 3.14?eV. TiO2:Nb nanopowders exhibited additional absorption in the visible range. In comparison to TiO2-P25, improved photocatalytic activity of TiO2:Nb was observed for the degradation of MB and MO under both UVA and VIS irradiation, where low doping level (Nb?<?1?at.%) was the most effective. Niobium doping affected structural, optical and photocatalytic properties of TiO2. Low dopant level enhanced photocatalytic performance under UVA and VIS irradiation. Therefore, TiO2:Nb (Nb?<?1?at.%) can be proposed as an efficient selective solar light photocatalyst. 相似文献
38.
A sensitive and reliable quantification method for Bisphenol A based on modified competitive ELISA method 总被引:1,自引:0,他引:1
Kim A Li CR Jin CF Lee KW Lee SH Shon KJ Park NG Kim DK Kang SW Shim YB Park JS 《Chemosphere》2007,68(7):1204-1209
Bisphenol A (BPA), generally known as bisphenols, has been identified as a potential estrogenic substance. BPA must be conjugated to carrier protein and BSA was commonly used. 4,4-Bis(4-hydroxyphenyl) valeric acid (BHPVA) has a bisphenolic structure and a long carbon chain with a reactive carboxyl group on the end. In this study, BHPVA-BSA was used to produce polyclonal antibody against bisphenolic structure, and a modified competitive ELISA method for quantification of BPA was developed. This system was based on BHPVA-BSA for polyclonal antibody production against bisphenolic structure, and BHPVA-HRP for determination of BPA substituting detection antibody in competitive reaction. Recovery was assessed at 10 different concentrations (2-1000 ng/ml) of BHPVA, and the recovery range was from 96.3% to 107.2%. The variation was from 6.2% to 9.8% for intra assay and from 10.1% to 12.6% for inter assay. The quadratic was used to establish the curve regression. The range was found to be between 2 and 1000 ng/ml. This modified competitive ELISA method has proven to be a very useful tool for quantification of BPA without the unexpected interaction of BSA and anti-BSA polyclonal antibody. 相似文献
39.
Chemical characteristics and sources of water-soluble organic aerosol in southwest suburb of Beijing
Ruolan Hu Qingcheng Xu Shuxiao Wang Yang Hua Noshan Bhattarai Jingkun Jiang Yu Song Kaspar R.Daellenbach Lu Qi Andre S.H. Prevot Jiming Hao 《环境科学学报(英文版)》2020,32(9):99-110
PM2.5 filter sampling and components measurement were conducted in autumn and winter from 2014 to 2015 at a suburban site(referred herein as "LLH site") located in the southwest of Beijing.The offline aerosol mass spectrometry(offline-AMS) analysis and positive matrix factorization(PMF) were applied for measurement and source apportionment of watersoluble organic aerosol(WSOA).Organic aerosol(OA) always dominated PM2.5 during the sampling period,especially in winter.WSOA po... 相似文献
40.
Noncrop areas such as hedgerows in agricultural landscapes can perform several ecological and agronomic functions (e.g., habitat,
movement corridors, windbreak, etc.), but their dynamics and drivers of changes are often poorly known. We conducted a study
in three agricultural landscapes of southern Quebec, Canada, to assess and compare the spatial and temporal (1958–1997) dynamics
of three hedgerow networks in relation to geomorphic conditions (marine, glacial, and mixed deposit) and land-use changes.
Hedgerow networks were mapped and described in terms of their structure (density, degree of connectivity, and presence of
trees or shrubs) and their relationship to other components of the landscape (connection to woodland). Relationships were
assessed in time and space using nonparametric correlation, Mantel test, and principal components analysis (PCA). Results
show significant differences between hedgerow structure for the three landscapes and distinct temporal and spatial dynamics
that can be related to changes in management practices and agricultural policies. On marine deposits, increases in hedgerow
density did not always correspond to an increase in their degree of connectivity, suggesting a possible reduction in network
quality. On glacial deposits, hedgerow density declined following abandonment of agricultural land, but rather than disappearing,
these linear structures were integrated into adjacent brush or forested areas. Our analysis reveals the complex spatial and
temporal dynamics of the hedgerow networks and highlights the need to take into account spatial attributes such as connectivity
and connection to woodland to evaluate more accurately overall network quality. 相似文献