离子色谱/质谱联用分析水和食品中的高氯酸盐

摘要用离子色谱／质谱联用(IC／MS)测定低ppb水平高氯酸根离子的方法．IC／MS是根据EPA方法314开发出来的,原方法用Ic分离电导检测,检测范围为1ppb．IC／MS在整个测定范围内,高氯酸盐回收率不受干扰基质的影响．在饮料和废水中0．5和1ppb添加水平的测定回收率为90％-105％,方法检测限(DML)低于100ppt．     

The impact of social media marketing strategies on promoting sustainability of tourism with fuzzy cognitive mapping: a case of Kalanggaman Island (Philippines)

Environment, Development and Sustainability - The current literature has espoused the role of social media platforms in promoting tourism destinations. Despite such advances, limited works are...     

Determination of steroidal estrogens in flushed dairy manure wastewater by gas chromatography-mass spectrometry

There is a critical need to accurately measure the concentrations of natural steroidal estrogens in flushed dairy manure wastewater (FDMW) to assess any potential risk of waterway contamination resulting from land application. Estrogens are a concern because low concentrations (10-100 ng L-1) in water can adversely affect aquatic vertebrate species such as fish, turtles, and frogs by disrupting the normal function of their endocrine systems. The objective of this study was to develop a sample preparation method that permits the quantification of four natural steroidal estrogens (17alpha-estradiol, 17beta-estradiol, estrone, and estriol) in FDMW by gas chromatography-mass spectrometry (GC-MS). Solid-phase extraction with graphitized carbon black was used for the bulk extraction of estrogens from FDMW and additional sample purification was accomplished with C-18. The sample preparation method allowed estrogens to be detected accurately by GC-MS in FDMW. Spiked recovery experiments indicated that the method is satisfactory for measuring the estrogens of interest in FDMW with average recovery of >90%. As expected in FDMW, characterization of the estrogen profile revealed a large abundance of 17alpha-estradiol relative to 17beta-estradiol and estrone. Estriol was not detected in FDMW. The methodology developed in this research helps provide an analytical foundation for the quantification of steroidal estrogens in FDMW by GC-MS.     

Fatty acid methyl ester analysis to identify sources of soil in surface water

Efforts to improve land-use practices to prevent contamination of surface waters with soil are limited by an inability to identify the primary sources of soil present in these waters. We evaluated the utility of fatty acid methyl ester (FAME) profiles of dry reference soils for multivariate statistical classification of soils collected from surface waters adjacent to agricultural production fields and a wooded riparian zone. Trials that compared approaches to concentrate soil from surface water showed that aluminum sulfate precipitation provided comparable yields to that obtained by vacuum filtration and was more suitable for handling large numbers of samples. Fatty acid methyl ester profiles were developed from reference soils collected from contrasting land uses in different seasons to determine whether specific fatty acids would consistently serve as variables in multivariate statistical analyses to permit reliable classification of soils. We used a Bayesian method and an independent iterative process to select appropriate fatty acids and found that variable selection was strongly impacted by the season during which soil was collected. The apparent seasonal variation in the occurrence of marker fatty acids in FAME profiles from reference soils prevented preparation of a standardized set of variables. Nevertheless, accurate classification of soil in surface water was achieved utilizing fatty acid variables identified in seasonally matched reference soils. Correlation analysis of entire chromatograms and subsequent discriminant analyses utilizing a restricted number of fatty acid variables showed that FAME profiles of soils exposed to the aquatic environment still had utility for classification at least 1 wk after submersion.     

Assessing motivation for work environment improvements: Internal consistency, reliability and factorial structure

Problem

Workers' motivation to actively take part in improvements to the work environment is assumed to be important for the efficiency of investments for that purpose. That gives rise to the need for a tool to measure this motivation.

Method

A questionnaire to measure motivation for improvements to the work environment has been designed. Internal consistency and test-retest reliability of the domains of the questionnaire have been measured, and the factorial structure has been explored, from the answers of 113 employees.

Results

The internal consistency is high (0.94), as well as the correlation for the total score (0.84). Three factors are identified accounting for 61.6% of the total variance.

Discussion

The questionnaire can be a useful tool in improving intervention methods.

Impact on Industry

The expectation is that the tool can be useful, particularly with the aim of improving efficiency of companies' investments for work environment improvements.     

Molecular Variation and Evolutionarily Significant Units in the Endangered Gila Topminnow

Determining evolutionarily significant units in endangered species is one of the most significant challenges facing conservation biology. Often genetic information has been used as the primary basis of recommendations for evolutionarily significant units, but these data should be evaluated carefully and used in conjunction with other information. The endangered Gila topminnow ( Poeciliopsis. o. occidentalis ) has been the subject of extensive conservation biology research and genetic investigation. We extended these data to highly variable genetic markers, examined variation in microsatellite loci, and compared it with previous measures of genetic diversity for the Gila topminnow from the four watersheds in Arizona in which they are still naturally extant. Fish from Monkey Spring were the most highly differentiated from the other populations. Overall, the amounts and patterns of genetic variation were consistent with known historical and physical differences among sites. The four watersheds are highly physically isolated from one another and differ in a number of important factors in their physical habitat, biota, and the life-history of the topminnows. Based on these geographic patterns and the genetic results, we recommend that the four watersheds all be managed and conserved separately.     

Interactions among plant defense compounds: a method for analysis

Summary. Plants contain an enormous diversity and quantity of secondary metabolites, some of which are toxic and deterrent to herbivores and pathogens. This impressive diversity of plant compounds suggests a high probability of interactions among them. Synergistic interactions are those in which the combined activity of two or more chemicals is greater than that expected given their individual activities. On the other hand, antagonistic interactions are those in which the combined activity of two or more chemicals is less than that expected given their individual activities. Synergistic interactions could increase plant fitness whereas antagonistic interactions could decrease plant fitness. Interactions are thus potentially very important, not only in explaining the diversity of defense compounds within individual plants, but also in providing insight into plant defense strategies. Although synergistic interactions have received increased attention in the ecological literature in the last decade, the number of documented cases of synergy remains small and antagonistic interactions are rarely considered. The primary reason for this scarcity may be the difficulty of detecting, analyzing and displaying such interactions. Analysis by ANOVA, though sometimes used, often is not appropriate. We introduce a simple technique, isobolographic analysis, that is used in pharmacology for detecting and rigorously quantifying synergy and antagonism and provide an example using the brine shrimp toxicity assay. More statistically sophisticated approaches, such as isobolographic analysis, will allow ecologists to effectively document the role of chemical synergy and antagonism in interactions between species. Such chemical interactions may ultimately provide insight into longstanding, ecological questions. Received 26 October 1998; accepted 24 March 1999.     

Demand for environmental policies to improve health: Evaluating community-level policy scenarios

Using a national survey and a discrete choice experiment format, we estimate demand for environmental polices to improve health. We use a richly detailed community-level approach that describes illnesses avoided, premature deaths avoided, policy duration, and the affected population size. We allow preferences for policy attributes to vary systematically with the scenario design, with the source of risk and type of health threat, and with respondent characteristics. Using a willingness to pay (WTP) framework similar to that used for studies of individual risk, we find that omission of illness information leads to an upward bias in estimates of the value of avoided premature deaths and that individuals view avoided deaths and avoided illnesses as substitutes. We also find evidence of strongly diminishing marginal utility in policy scope. Differences in marginal WTP from different sources of risk or types of illness appear very small relative to differences associated with respondent characteristics and/or perceptions. Self-interest strongly dominates altruistic considerations.     

Vigilance and aggregation in black howler monkeys (Alouatta pigra)

Animals are expected to reduce per capita vigilance with increasing group size. Widespread support for this expectation has been found in studies of birds and ungulates. Primate vigilance patterns appear to differ, but studies of primates may have sampled groups with too many members to detect intergroup differences in vigilance. We tested this idea by measuring individual vigilance rates in wild black howler monkeys (Alouatta pigra) living in stable groups of two to ten individuals. No group size effect on vigilance emerged. As in previous studies, inter-individual proximity explained variation in individual vigilance rates. Focal animals with associates within 2 m and with multiple associates in the same tree were less vigilant than isolated animals. Activity and class of focal animals as well as the presence of neonates influenced the average vigilance of adult and subadult black howler monkeys. We conclude that functional explanations must be considered for the general lack of a group size effect on primate vigilance.     

Consensus versus Conservation in the Upper Colorado River Basin Recovery Implementation Program

Abstract: We examined consensus-based management through the lens of the Colorado River Recovery Implementation Program, a consensus-based plan that attempts to develop the Colorado River's water while protecting its endangered fishes. Because this management model has been touted as a preferred substitute to government-imposed regulation, we analyzed the recovery implementation program to determine its strengths and weaknesses. By reviewing secondary information and interviewing members of the diverse groups involved in the program, we gathered detailed information about the program's history, implementation, and progress. Our investigation revealed that the recovery implementation program has allowed development of the Colorado River's water and incorporated more voices into the decision-making process. But the program circumvented federal authority over endangered species conservation, which has proved detrimental to the fishes. Furthermore, we learned that the consensus-based model is vulnerable to control by special-interests and may be driven by bureaucratic procedural goals rather than species recovery. To ameliorate these concerns, (1) program success should be judged by species recovery, rather than political achievements, (2) the federal government should retain the power of issuing statutory sanctions in the event of continued population decline, and (3) funding should be provided by an agency with a clear species-protection agenda to reduce the disproportionate power of utilitarian interest groups. By incorporating these recommendations, conservation programs can better realize the benefits of a consensus-based approach without sacrificing species recovery.