Rapid one-step assays for on-site monitoring of mouse and rat urinary allergens

Allergy to rodent proteins is common among laboratory animal workers. Sensitive methods to measure exposure to these allergens have been developed. These assays are, however, expensive, time-consuming, and require a laboratory facility and methodological expertise. A simple method to screen for allergen spread, or to test whether hygiene standards are maintained, would be useful. Lateral flow immunoassays (LFIAs) are especially suited for field settings; the tests are simple and results are visible within minutes. LFIAs were developed for detection of the rodent urinary allergens Mus m 1 and Rat n 1. Pilot studies were performed in animal facilities in three countries using both extracts from airborne dust samples and samples collected by wiping surfaces. For comparison and determination of sensitivity, the concentrations of rodent urinary allergens in the samples were also measured using enzyme immunoassays (EIAs). The LFIAs for rat and mouse urinary allergens had a detection limit of 31 pg allergen per mL in a buffer system with purified allergen standards. Results of environmental dust extracts tested in LFIAs correlated well with levels obtained using EIAs. Spread of rodent allergens, or non-adherence to hygiene around laboratory animal facilities, may aggravate rodent allergy. Using a simple, sensitive one-step assay, allergens can be detected to prevent allergen exposure. The results reveal that the rapid assays are suited for on-site demonstration of exposure to rodent allergens, and thus, useful in occupational hygiene practice.     

Dependability assessment of network-based safety-related system

Advances in electronic integration and radio communication have led to the emergence of a new kind of safety systems, i.e. Wireless Sensor Network (WSN). This network-based safety-related system is becoming more and more present in the domain of safety due to its easy deployment. It does not need a wire infrastructure and its range of applications is wide. Usually, such a system is composed of various nodes (sensors) collaborating to monitor a targeted phenomenon. In most cases, nodes are battery powered and this is the weakness of the system makes it necessary to design an energy saving policy. The present paper gives a dependability viewpoint of such a system. A modeling framework is suggested integrating the interdependency of the components. Stochastic Petri nets are used to implement this model and two heuristics to schedule component activity. The first is based on the proposed importance measures, the second on a multi-objective genetic algorithm. The aim is to conserve energy and so to extend WSN dependability.     

The practice of valuing the environment in cost-benefit analyses in transport and spatial projects

Scientific debates on environmental impacts in cost-benefit analysis (CBA) focus on methodological questions. The literature, however, contains very little information on the influence of these debates on CBAs made in spatial planning practice. In this paper, this gap is filled by a qualitative analysis of 67 CBAs made in the Netherlands between 2000 and 2011. From the literature on environmental valuation, we derive criteria to evaluate the quality of CBA practice, such as completeness and the inclusion of uncertainties. The analysis shows that in many cases relevant environmental effects are omitted or not monetised. Moreover, non-monetised effects are often not included in CBA conclusions. If impacts are monetised, the methods used are frequently not very sophisticated. The consequences of assumptions are often not communicated, for example, where high discount rates make long-term effects seem unimportant. In this research we identify four main points for good practice.     

Behavioural Reactivation and Subjective Assessment of the State of Vigilance—Application to Simulated Car Driving

The frequency of some behaviour (such as self-centred gestures) increases during a task that leads to the occurrence of low-vigilance episodes. These gestures can be useful in stimulating oneself. A study carried out in 20 adults has enabled us to state that motor activity (recorded with an actimeter) increases with the duration of a monotonous driving task and sleep deprivation. The analysis of the scores recorded using the Karolinska Sleepiness Scale has shown that drivers can assess the deterioration of their state of vigilance according to the actual sleep preceding the driving test. Finally, the joint analysis of the subjective and objective data revealed a co-variation of these two types of indices. We discuss the stimulatory function of the motor activity in a task leading to the occurrence of low-vigilance episodes by investigating, among other things, the use, conscious or not, of this type of activity.     

Living without buffers—illustrating climate vulnerability in the Lake Victoria basin

Exposure, sensitivity and adaptive capacity are essential, albeit theoretically vague, components of climate vulnerability. This has triggered debate surrounding how these factors can be translated into, and understood in, an empirical context subject to present and future harm. In this article, which draws on extensive fieldwork in the Lake Victoria Basin of Kenya and Tanzania, we illustrate how exposure, sensitivity and adaptive capacity play out in the context of climate vulnerability and discuss how they interact in situ. Using a mixed methods approach including survey data, rainfall data and a suite of participatory methods, such as focus groups and interactive mapping of seasonal calendars, we identify how climate-induced stressors affect smallholder farmers’ well-being and natural resources. Drawing on the seasonal calendar as a heuristic, and climate vulnerability terminology, we illustrate when, where and how these climate-induced stressors converge to constrain farmers’ livelihoods. Our analysis indicates that farmers in the basin face a highly uncertain future with discernible, but differentiated, adaptation deficits due to recurring, and potentially worsening, patterns of hardship.