Flow cytometry detection of lysosomal presence and lysosomal membrane integrity in the three-spined stickleback (Gasterosteus aculeatus L.) immune cells: applications in environmental aquatic immunotoxicology.

The neutral red retention assay has been proposed to determine the lysosomal membrane stability in immune cells. Nevertheless, this assay implies many examinations under a microscope at short time intervals and therefore the analysis of few samples. The present study proposes two more rapid, efficient, and sensitive sample analyses using flow cytometry method. Lysosomal presence and lysosomal membrane integrity (LMI) were evaluated on the three-spined stickleback, Gasterosteus aculeatus (L.), a well-described model fish species for aquatic ecotoxicology studies. After development of the two biomarkers, they were validated by ex vivo contamination with endosulfan and copper and by in situ sampling. These immunomarkers were clearly modulated by pollutants and their variations seemed to be correlated with leucocyte mortality. Thus, from a practical point of view, lysosomal presence and LMI may provide novel and efficient means of evaluating immune capacities and indicating the toxic effects of environmental pollution.     

Thermal strain in fire fighters while wearing task-fitted versus en 469:2005 protective clothing during a prolonged rescue drill.

Fire fighters are normally overprotected during their working hours because of the tendency to keep the personal protection level sufficiently high in case of the worst possible scenarios. This study investigated the effects of task-fitted protective clothing on thermal strain in fire fighters as compared to EN 469:2005 protective clothing during a prolonged (2 1/2 hrs) job-related rescue drill under neutral and hot climates. The subjects were 23 healthy, physically fit professional male fire fighters aged 26-44 years. Measurements included cardiovascular and thermal responses and subjective assessments. Wearing task-fitted clothing during rescue tasks in a neutral climate considerably reduced total thermal and cardiovascular strain in prolonged rescue work. The fire fighters also perceived physical work as significantly harder on average, and reported more intense subjective discomfort while wearing EN 469:2005 as compared to task-fitted clothing.     

Dispersion of 222Rn from two New Zealand geothermal power plants

The dispersion of ²²²Rn from emitted waste gases at Wairakei geothermal power station, New Zealand, is modelled. It is concluded that resulting concentrations in the nearby township of Taupo will never exceed the maximum permissible in any meteorological situation. The greatest possible accumulation is calculated to be less than one eighth of the normal background radon concentration. A more realistic set of assumptions predicts long-term mean concentrations about 4% of background levels. A new geothermal power station, Ohaaki, a factor of three times more distant, is calculated to produce ten times lower concentrations than Wairakei. Measurements using a few passive solid-state radon detectors show that the natural variation of radon concentrations greatly exceeds any calculated contribution from either geothermal station; hence, much of the radon is probably due to more local minor sources. Local sources have increased due to draw-off of ground water by the Wairakei station. Thus, there could be some indirect contribution to radon concentrations by its operation. The measured six-month integrated mean radon concentration at Taupo is a significant fraction of the maximum permissible concentration. It seems likely that natural sources alone may lead to levels in air which are above the maximum when temperature inversion exists. It is concluded that these two geothermal power plants are unlikely to produce concentrations of radon hazardous to the population or to plant workers.     

A system-based approach to the genetic etiologies of non-immune hydrops fetalis

A wide spectrum of genetic causes may lead to nonimmune hydrops fetalis (NIHF), and a thorough phenotypic and genetic evaluation are essential to determine the underlying etiology, optimally manage these pregnancies, and inform discussions about anticipated prognosis. In this review, we outline the known genetic etiologies of NIHF by fetal organ system affected, and provide a systematic approach to the evaluation of NIHF. Some of the underlying genetic disorders are associated with characteristic phenotypic features that may be seen on prenatal ultrasound, such as hepatomegaly with lysosomal storage disorders, hyperechoic kidneys with congenital nephrosis, or pulmonary valve stenosis with RASopathies. However, this is not always the case, and the approach to evaluation must include prenatal ultrasound findings as well as genetic testing and many other factors. Genetic testing that has been utilized for NIHF ranges from standard chromosomal microarray or karyotype to gene panels and broad approaches such as whole exome sequencing. Family and obstetric history, as well as pathology examination, can yield additional clues that are helpful in establishing a diagnosis. A systematic approach to evaluation can guide a more targeted approach to genetic evaluation, diagnosis, and management of NIHF.     

Soil microbial response to waste potassium silicate drilling fluid

Potassium silicate drilling fluids (PSDF) are a waste product of the oil and gas industry with potential for use in land reclamation. Few studies have examined the influence of PSDF on abundance and composition of soil bacteria and fungi. Soils from three representative locations for PSDF application in Alberta, Canada, with clay loam, loam and sand textures were studied with applications of unused, used once and used twice PSDF. For all three soils, applying ≥ 40 m³/ha of used PSDF significantly affected the existing soil microbial flora. No microbiota was detected in unused PSDF without soil. Adding used PSDF to soil significantly increased total fungal and aerobic bacterial colony forming units in dilution plate counts, and anaerobic denitrifying bacteria numbers in serial growth experiments. Used PSDF altered bacterial and fungal colony forming unit ratios of all three soils.     

The role of natural disaster in individual and relational adjustment in Sri Lankan mothers following the 2004 tsunami

The purpose of this study is to examine the associations between maternal mental health distress symptoms, including depression and post‐traumatic stress disorder, the extent to which the presence of a child's disaster‐related physical health problem(s) have interfered with daily functioning, and family cohesion over time among Sri Lankan mothers who survived the tsunami on 26 December 2004. Study variables were measured using a self‐report questionnaire administered approximately four months after the event and three years later in summer 2008. Univariate, bivariate, and multivariate analyses were conducted. Path analysis was employed to assess the relationships between the key variables over time and the correlations in the study variables at each time point. Among other findings, the results of the path analysis indicated that post‐traumatic stress symptom distress four months after the disaster significantly predicted variance in family cohesion three years later. Clinical and empirical research implications are presented and discussed.