Molecular insight into the population structure of common and spotted dolphins inhabiting the pelagic waters of the Northeast Atlantic

Several cetacean species exhibit fine-scale population structure despite their high dispersal capacities and the apparent continuity of the marine environment. In dolphins, most studies have focused on coastal areas and continental margins, and they revealed differentiated populations within relatively small geographic areas, sometimes in conjunction with a specialisation for different habitats (ecotypes). We analysed the population genetic structure of short-beaked common dolphins (Delphinus delphis) and Atlantic spotted dolphins (Stenella frontalis) in the Azores and Madeira, the two most isolated archipelagos of the North Atlantic. The archipelago of the Azores is divided into three groups of islands and stands 900 km away from Madeira. It is not known whether individuals migrate between groups of islands and archipelagos, nor whether distinct ecotypes are present. These questions were investigated by genetic analyses of 343 biopsy samples collected on free-ranging dolphins. The analyses consisted in sequencing part of the mitochondrial hyper-variable region, screening up to 14 microsatellite loci, and molecular sexing. Results did not unravel any population structure at the scale of the study area. Lack of differentiation matches expectations for spotted dolphins, which are transient in both archipelagos, but not for common dolphins, which are present year-round in the Azores and potentially resident. Absence of genetic structure over hundreds and even thousands of kilometres implies the existence of gene flow over much larger distances than usually documented in small delphinids, which could be achieved through individual movements. This finding indicates that population structure in oceanic habitat differs from that observed in coastal habitat.     

A three-isotope approach to disentangling the diet of a generalist consumer: the yellow-legged gull in northwest Spain

The widespread omnivory of consumers and the trophic complexity of marine ecosystems make it difficult to infer the feeding ecology of species. The use of stable isotopic analysis plays a crucial role in elucidating trophic interactions. Here we analysed δ¹⁵N, δ¹³C and δ³⁴S in chick feathers, and we used a Bayesian triple-isotope mixing model to reconstruct the diet of a generalist predator, the yellow-legged gull (Larus michahellis) that breeds in the coastal upwelling area off northwest mainland Spain. The mixing model indicated that although chicks from all colonies were fed with a high percentage of fish, there are geographical differences in their diets. While chicks from northern colonies consume higher percentages of earthworms, refuse constitutes a more important source in the diet of chicks from western colonies. The three-isotope mixing model revealed a heterogeneity in foraging habitats that would not have been apparent if only two stable isotopes had been analysed. Moreover, our work highlights the potential of adding δ³⁴S for distinguishing not only between terrestrial and marine prey, but also between different marine species such as fish, crabs and mussels.     

Estimating the influence of overhead transmission power lines and landscape context on the density of little bustard Tetrax tetrax breeding populations

Collision with conductors and earth cables is a known impact generated by transmission power lines, however there is virtually no information on how these infrastructures might affect bird distribution in a landscape context. With this work we specifically hypothesise that transmission power lines may affect the occurrence of a threatened bird, the little bustard (Tetrax tetrax). To test this hypothesis we used a Stochastic Dynamic Methodology (StDM), analysing the effects of power lines in a landscape perspective and simulating population trends as a response to power line installation and habitat changes induced by agricultural shifts in southern Portugal. The data used in the dynamic model construction included relevant gradients of environmental conditions and was sampled during the breeding seasons of 2003-2006. Transmission power lines were significantly avoided by the little bustard and the developed StDM model showed that the distance to these utility structures is the most important factor determining breeding densities in sites with suitable habitat for the species, which possibly leads to displacement of populations and habitat fragmentation. The model simulations also provided the base to analyse the cumulative effects caused by the habitat degradation that can ultimately lead to the extinction of local populations. Within priority conservation sites, the dismantling of existing transmission lines should be considered whenever possible, in order to ensure adequate breeding habitat. The model is considered useful as an auxiliary tool to be used in environmental impact assessments, management and conservation studies.     

Spatial extremes of wildfire sizes: Bayesian hierarchical models for extremes

In Portugal, due to the combination of climatological and ecological factors, large wildfires are a constant threat and due to their economic impact, a big policy issue. In order to organize efficient fire fighting capacity and resource management, correct quantification of the risk of large wildfires are needed. In this paper, we quantify the regional risk of large wildfire sizes, by fitting a Generalized Pareto distribution to excesses over a suitably chosen high threshold. Spatio-temporal variations are introduced into the model through model parameters with suitably chosen link functions. The inference on these models are carried using Bayesian Hierarchical Models and Markov chain Monte Carlo methods.     

Effects of atrazine and nicosulfuron on freshwater microalgae

Growth modifications caused by various concentrations of atrazine and nicosulfuron were monitored in closed and continuous culture of Chlorella vulgaris (chlorophyta), Navicula accommoda (diatomophyta), and Oscillatoria limnetica (cyanophyta). The concentration at which algal growth rate was reduced twofold (EC50) was determined in the three species for both herbicides. Comparatively, the two toxicants were applied at 10 microg/l level in microcosms inoculated with natural phytoplankton from Lake Geneva. The relative abundances of major phytoplanktonic species were measured by algal cell count at the beginning and at the end of each experiment. Atrazine and nicosulfuron have different targets in plant metabolism, respectively, photosystem II (PSII) and acetolactate synthase (ALS), and the expected effects were different. Generally, the cultured phytoplankton exhibited various sensitivities, depending on species or herbicide. In the microcosms, the major taxa of natural phytoplanktonic samples exhibited various patterns, from acute toxicity to growth enhancement. For example, the diatoms inside the community were not affected by atrazine and nicosulfuron, except for Stephanodiscus minutulus that was sensitive to both, and Asterionella/formosa that was sensitive only to nicosulfuron. The specific physiology and the relationships among the phytoplanktonic communities have to be carefully considered when one would try to predict the extent of herbicide action on natural phytoplankton using in vitro tests. There is a need to test the toxic effect on various cultured strains, representative of most of the taxonomic composition of natural communities, to take into account the wide range of sensitivities and reaction to herbicide contamination. But this is not enough to give a solid frame when transposing the results to the field, and the use of more ecologically relevant systems is recommended.     

Recycling glass-polishing sludge and aluminum anodising sludge in polyurethane and cement composites: fire performance and mechanical properties

Journal of Material Cycles and Waste Management - This article aims to study the mechanical strength and fire resistance of polyurethane/cement (PuCem) composites containing glass sludge and sludge...     

Exploring the Potential of Dutch Pig Farmers and Urban-Citizens to Learn Through Frame Reflection

The Dutch pig husbandry has become a topic of public debate. One underlying cause is that pig farmers and urban-citizens have different perspectives and underlying norms, values and truths on pig husbandry and animal welfare. One way of dealing with such conflicts involves a learning process in which a shared vision is developed. A prerequisite for this process is that both parties become aware of their own fixed patterns of thoughts, actions, and blind spots. Therefore, we conducted five homogeneous focus groups consisting of either urban-citizens or pig farmers. The first part of the sessions aimed to make the participants aware of their own perspectives and underlying norms and values concerning animal welfare, farm practices or consumer behavior. Then, by the use of role-play and film fragments showing the perspectives of the other party, we aimed to stimulate frame reflection. The farmers maintained their own perspective and defended their practices. They denied the perspective of the urban-citizens by portraying them as ignorant of the “factual” farm practices. They proposed the use of one-way information, but our results indicate that this is likely to fail as a strategy to support or restore public acceptance. Our case shows hardy any consensus regarding the relevance of the facts at stake and a very limited amount of shared values. However, the shared love for animals together with the recognition by the urban-citizens of the inescapable dilemma for farmers to adopt a use-framing towards animals might provide an opening for further learning strategies.     

Meeting Heterogeneity in Consumer Demand for Animal Welfare: A Reflection on Existing Knowledge and Implications for the Meat Sector

The legitimacy of the dominant intensive meat production system with respect to the issue of animal welfare is increasingly being questioned by stakeholders across the meat supply chain. The current meat supply is highly undifferentiated, catering only for the extremes of morality concerns (i.e., conventional vs. organic meat products). However, a latent need for compromise products has been identified. That is, consumer differences exist regarding the trade-offs they make between different aspects associated with meat consumption. The heterogeneity in consumer demand could function as a starting point for market segmentation, targeting and positioning regarding animal welfare concepts that are differentiated in terms of animal welfare and price levels. Despite this, stakeholders in the meat supply chain seem to be trapped in the dominant business model focused on low cost prices. This paper aims to identify conflicting interests that stakeholders in the meat supply chain experience in order to increase understanding of why heterogeneous consumer preferences are not met by a more differentiated supply of meat products produced at different levels of animal welfare standards. In addition, characteristics of the supply chain that contribute to the existence of high exit barriers and difficulty to shift to more animal-friendly production systems are identified. Following the analysis of conflicting interests among stakeholders and factors that contribute to difficulty to transform the existing dominant regime, different routes are discussed that may help and motivate stakeholders to overcome these barriers and stimulate the creation of new markets.     

Field-breeding birds on organic and conventional arable farms in the Netherlands

In this study territory densities of field-breeding farmland birds were compared on pairwise-selected organic and conventional arable farms for two years. Differences in territory densities between the two farm types were explained examining the effects of three factors on territory densities: (1) non-crop habitats, (2) crop types and (3) within-crop factors. In both years, densities of most species did not differ between organic and conventional farms. Only skylark and lapwing were more abundant on organic farms, but only skylarks showed a consistent pattern over both years. Differences in crop types grown between the two systems were the only explaining factor for differences in densities of skylark. For lapwing, the difference was only partly due to differences in crop type, but differences in within-crop factors (probably as a result of crop management) were likely to have had an effect as well. There were no significant differences in abundance of non-crop habitats between the two farming systems, so this could not explain differences in territory densities.     

A new approach to assessment and management of the impact from medical liquid radioactive waste

The Swedish regulations concerning disposal of clinical radioactive waste are currently under revision and a graded approach is proposed for risk limitation purposes. To assist the revision procedures, a screening study was performed to estimate public exposures from liquid releases from hospitals to public sewers. The results showed that doses to sewage workers were above the dose constraint of 100muSva(-1) especially for (131)I and (99m)Tc. Hence, a dynamic model, LUCIA, was developed for realistic assessments in which radionuclide transportation in sewers was modelled. Probabilistic simulations were performed to obtain probability distributions of radionuclide concentrations in sludge. Concurrently, estimates of the effective doses to sewage workers decreased significantly and were below 10muSva(-1) except for (111)In and (131)I. However, the Kd-coefficients representing the partition of radioactivity between water and sludge in sewers are highly uncertain for (111)In. As shown by sensitivity studies, these values are the major determinant of the exposures in sewers.