Heterogeneous energetic pathways and carbon sources on deep eastern Mediterranean cold seep communities

Cold seep communities in the Mediterranean Sea have only been discovered two decades ago, and their trophic ecology has been the subject of very few studies. We investigated the benthic food web of two deep chemosynthesis-based ecosystems on the Napoli and Amsterdam mud volcanoes (MVs) in the eastern Mediterranean Sea (~2,000 m depth). Seeping methane has been detected at the surface of both MVs during pioneering cruises and has been hypothesised to be assimilated by benthic fauna as observed in other oceans’ margins. Given the extreme oligotrophic character of the eastern Mediterranean Sea, we a priori expected that chemosynthetic food sources, especially methane-derived carbon (MDC), played a major trophic role in these deep seep communities relative to what has been observed in other seep systems worldwide. We aimed at unravelling the trophic relationships on Napoli and Amsterdam MVs through the analysis of carbon, nitrogen and sulphur isotopes both in the dominant benthic invertebrates including the small endofauna (300 μm < size < 1 cm) and in the sedimented organic matter. In particular, we assessed the fraction of MDC in the tissue of several heterotrophic and symbiotic species. Low mean δ³⁴S and δ¹³C values (0.4 ± 4.8‰ and −31.6 ± 5.7‰, respectively) obtained for mega- and macrofauna suggested that the investigated benthic food webs are virtually exclusively fuelled by carbon of chemosynthetic origin. A few grazer invertebrates (δ³⁴S up to 11‰) depart from this trend and could complement their diet with sedimented and decayed phytoplanktonic organic matter. Faunal δ¹³C values indicated that the oxidation of sulphur is likely the predominant energetic pathway for biosynthesis on both MVs. Nevertheless, mytilid bivalves and small capitellid, ampharetid and spionid polychaetes were ¹³C-depleted (δ¹³C < −37‰) in a way indicating they assimilated a significant portion of MDC. For these later heterotrophic species, MDC ranged between 21 and 31% (lower estimates) and 97 and 100% (upper estimates). However, our results highlighted that the origin of assimilated carbon may be complex for some symbiotic species. The vestimentiferan tubeworm Lamellibrachia sp., which exclusively depends on its sulphur-oxidising endosymbionts, showed a ~20‰ inter-individual δ¹³C variability on a very small spatial scale (<1 m) at the summit of Napoli MV. This mostly reflects the variable isotopic composition of pore-water-dissolved inorganic carbon (DIC) and evidenced that tubeworms (and subsequently their endosymbionts) uptake DIC derived from multiple methane oxidation processes in varying proportions. The lower and upper MDC estimates for the vestimentum of Napoli’s individuals were 11–38 and 21–73%, respectively. Finally, data on trophic ecology of Napoli and Amsterdam MVs clearly corroborate previous geophysical results evidencing the spatial heterogeneity of Mediterranean MV environmental conditions.     

生态保护学的建设与发展

通过简要回顾生态保护工作的发展历程,将生态保护学划分为自然保护学和生态保护学两个阶段。从研究对象及性质、内容与分支、学科基础、学科体系,论述了《环境保护学》的建设和发展,并指明了生态保护学的地位、作用及发展趋势。     

Does Organic Farming Face Distinctive Livestock Welfare Issues? – A Conceptual Analysis

The recent development and growth oforganic livestock farming and the relateddevelopment of national and internationalregulations has fueled discussions amongscientists and philosophers concerning theproper conceptualization of animal welfare.These discussions on livestock welfare inorganic farming draw on the conventionaldiscussions and disputes on animal welfare thatinvolve issues such as different definitions ofwelfare (clinical health, absence of suffering,sum of positive and negative experiences,etc.), the possibility for objective measuresof animal welfare, and the acceptable level ofwelfare. It seems clear that livestock welfareis a value-laden concept and that animalwelfare science cannot be made independent ofquestions of values and ethics. The questioninvestigated here is whether those values thatunderpin organic farming, in particular, alsoaffect the interpretation of livestock welfare,and, if so, how. While some of the issuesraised in connection with organic farming arerelatively uncontroversial, others are not. Theintroduction of organic farming values seems tointroduce new criteria for what counts as goodanimal welfare, as well as a different ethicalbasis for making moral decisions on welfare.Organic farming embodies distinctive systemicor communitarian ethical ideas and the organicvalues are connected to a systemic conceptionof nature, of agriculture, of the farm, and ofthe animal. The new criteria of welfare arerelated to concepts such as naturalness,harmony, integrity, and care. While the organicvalues overlap with those involved in theconventional discussion of animal welfare, someof them suggest a need to set new prioritiesand to re-conceptualize animal welfare – forexample, with respect to ``naturalness,' inrelation to the possibilities for expression ofnatural behavior and in relation to animalintegrity as a concept for organismic harmony.The organic perspective also seems to suggest awider range of solutions to welfare problemsthan changes in farm routines or operations onthe animals. The systemic solutions include thechoice and reproduction of suitable breeds,changes in the farm structure, and changes inthe larger production and consumption system – including consumer perceptions andpreferences. But the organic values may alsocall for sacrifices of individual welfare in aconventional sense in order to advance welfarefrom the perspective of organic farming.Whether this is good or bad cannot be decidedwithout entering into an inquiry and discussionof the values and ethics involved.     

Some requirements of an interactive software tool for life cycle analysis

This paper briefly presents the current practice for carrying out life cycle inventories, and mentions some advantages and drawbacks of a few dedicated software tools that are commercially available. A list is presented of some features that we think should be present in an ‘ideal’ life cycle analysis (LCA) software tool, after the specifications made at Ecobilan for the development of a specific tool for LCA. Some consideration is given to the technical difficulties involved, and the benefits that could be expected from the implementation of such features.     

Soy production and certification: the case of Argentinean soy-based biodiesel

With the rising emphasis on biofuels as a potential solution to climate change, this paper asks whether certification schemes, developed to promote sustainable feedstock production, are able to deliver genuine sustainability benefits. The Round Table on Responsible Soy (RTRS) is a certification scheme that aims to promote responsible soy production through the development of principles and criteria. However, can and does this initiative address the negative impacts associated with the intensive production of soy? Taking the example of soy biodiesel produced in Argentina, this paper asks whether the social and environmental impacts of soybean production can be mitigated by the RTRS. It concludes that at present certification schemes are unlikely to be able to address either the institutional challenges associated with their implementation or the detrimental impacts of the additional demand generated by biofuels.     

城市污水水质水量变化及生活污染源污染特征研究

在收集西安市污水处理厂近年来水质水量监测数据的基础上,统计分析污水处理厂水量水质的变化规律,并通过对不同生活污水污染源的现场监测,研究各类生活污水污染源的污染强度和污染特征,识别重点生活污染源。研究结果表明,西安市主要污水处理厂近年来进水量逐年增大,且进水水质逐年恶化,其中COD、SS和NH3-N浓度逐年增加,TP浓度年际变化较小;城市污水的水质水量随季节波动变化,西安市城市污水水量以及COD、SS和NH3-N等水质指标在7-9月份及春节期间出现高峰,这与季节及年节导致的用水量变化有关,但年内TP浓度均在10 mg/L以下,波动较小;生活类污染源中污染物浓度因行业不同而异,餐饮业、学校、住宿业及生活小区是生活类污染源中的＂高污染＂行业,加强对上述行业的监管力度,从源头消减污染物对于节能减排具有重要的现实意义。     

Double-outlet right ventricle with absent left ventricle and mitral atresia in a fetus with a deletion 22q12

Interstitial deletions of chromosomal region 22q12 are rare. We report the prenatal diagnosis of a de novo interstitial deletion 22q12. The fetus was karyotyped because of a complex cardiac anomaly. Conventional and molecular cytogenetics showed a female karyotype with a de novo pericentric inversion of one chromosome 22 associated with a deletion of the chromosomal region 22q12 leading to a partial monosomy 22q12. At autopsy, the fetus showed double-outlet right ventricle (DORV) with absent left ventricle and mitral atresia. This observation suggests that one or several genes for the early looping step of heart development may reside in chromosomal region 22q12. Further studies are needed to identify these genes, and to search microdeletions of 22q12 region in patients with DORV. Copyright © 2004 John Wiley & Sons, Ltd.     

Model of stomatal ammonia compensation point (STAMP) in relation to the plant nitrogen and carbon metabolisms and environmental conditions

Agricultural crops can be either a source or a sink of ammonia (NH₃). Most NH₃ exchange models developed so far do not account for the plants nitrogen (N) metabolism and use prescribed compensation points. We present here a leaf-scale simplified NH₃ stomatal compensation point model related to the plants N and carbon (C) metabolisms, for C₃ plants. Five compartments are considered: xylem, cytoplasm, apoplasm, vacuole and sub-stomatal cavity. The main processes accounted for are the transport of ammonium (NH₄⁺), NH₃ and nitrate (NO₃⁻) between the different compartments, NH₄⁺ production through photorespiration and NO₃⁻ reduction, NH₄⁺ assimilation, chemical and thermodynamic equilibriums in all the compartments, and stomatal transfer of NH₃.The simulated compensation point is sensitive to paramaters related to the apoplastic compartment: pH, volume and active transport rate. Determining factors are leaf temperature, stomatal conductance and NH₄⁺ flux to the leaf. Atmospheric NH₃ concentration seem to have very little effect on the compensation point in conditions of high N fertilization. Comparison of model outputs to experimental results show that the model underestimates the NH₃ compensation point for high N fertilization and that a better parametrisation of sensitive parameters especially active trasport rate of NH₄⁺ may be required.     

Stability studies of cholesterol lowering statin drugs in aqueous samples using HPLC and LC–MS

In this study, stability of statin drugs in different conditions, such as various pH, diverse solvents ratio, presence of UV, and sunlight have been investigated. Results suggest strong dependence of statins upon pH, potential environmental persistence towards sun light, and UV light degradation via singlet excited state obtained by excitation into the π–π* band. In acidic conditions interconversion between lactone and hydroxy acid forms in aqueous solutions at room temperature is retarded, while for the same sun-exposed samples are accelerated. Longer exposures lead to the degradation processes. Statin interconversion in water is much lower than in acetonitrile.