The amplification refractory mutation system (ARMS): A rapid and direct prenatal diagnostic technique for β-thalassaemia in Singapore

β-Thalassaemia major patients have chronic anaemia and since 3–4 per cent of Singaporeans carry the β-gene, prenatal diagnosis is essential. We evaluated the amplification refractory mutation system (ARMS) technique as a routine test for prenatal diagnosis of β-major. Six mutations along the β-gene were studied—41–42 (-TCTT), IVSII #654 (C-T), 17β (A-T), – 28 TATA (A-G), IVSI #5 (G-C), and IVSI #1 (G-T). Our results indicate that prenatal diagnosis using these mutations can be offered to 90 per cent (35/39) of our Chinese couples and 54·6 per cent (12/22) of our Malay couples at risk. Confirmation of ARMS results was carried out using allele-specific oligonucleotide hybridization. Prenatal diagnosis using ARMS was successfully carried out in nine cases which included a set of triplets and twins. The triplets were diagnosed with the β-trait carrying the 41–42 mutation. The couple with twins possessed the #654 mutation and one twin was diagnosed with the β-trait and the other with #654 homozygosity. Genomic sequencing of the undefined mutations in the Chinese couples revealed rarer mutations at − 29 and an ATG-AGG base substitution at the initiation codon for translation. In the Malay couples, genomic sequencing detected mutations at codon 15 (TGG-TAG) and codon 26 (GAG-AAG). We conclude that ARMS with its direct detection of amplified products by gel electrophoresis provides an accurate, rapid, and simpler method for our β-thalassaemia prenatal diagnosis programme in Singapore.     

Is there a commercially viable market for crop insurance in rural Bangladesh?

The study aims to assess the commercial viability of a potential crop insurance market in Bangladesh. In a large scale household survey, agricultural farm households were asked for their preferences for a hypothetical crop insurance scheme using double bounded (DB) contingent valuation (CV) method. Both revenue and production cost based indemnity payment approaches were applied to assess the commercial viability of a crop insurance program assuming a partner-agent (PA) model of insurance supply. Crop insurance is found marginally commercially viable in riverine flood plain areas. The expected indemnity payable consistently exceeds the expected insurance premium receivable by the insurer for the households living in wetland basin and coastal floodplain. We conclude that a uniform structure of crop insurance market does not exist in Bangladesh. The nature of the disaster risks faced by the farm households and the socio-economic characteristics of the rural farm communities need to be taken into careful consideration while designing such an insurance scheme.

The effects of Environmental Kuznets Curve toward environmental pollution,energy consumption on sustainable economic growth through moderate role of technological innovation

Environmental Science and Pollution Research - South Asia is a hub for encompassing air contamination, with 37 of the top tiers of the 40 most contaminated urban communities around the globe...     

Recovery of gas waste from the petroleum industry: a review

Environmental Chemistry Letters - Globally, 150–170 billion cubic metres of gas are being flared annually from petroleum refineries, petrochemical industries and from landfills. In this...     

An efficient neural network model for aiding the coagulation process of water treatment plants

Environment, Development and Sustainability - In a water treatment plant, the decision to carry out a jar test, for determining the required coagulant dosage, is made based on notable changes in...     

Correction to: The effects of Environmental Kuznets Curve toward environmental pollution,energy consumption on sustainable economic growth through moderate role of technological innovation

Environmental Science and Pollution Research -     

Sustainability and mechanical property assessment of concrete incorporating eggshell powder and silica fume as binary and ternary cementitious materials

Environmental Science and Pollution Research - Cement production emits a significant carbon dioxide (CO2) gas, dramatically influencing the environment. Furthermore, a large amount of energy is...     

A pathway to involve consumers for exchanging electronic waste: a deep learning integration of structural equation modelling and artificial neural network

Journal of Material Cycles and Waste Management - The pandemic of COVID-19 has disrupted every human life by putting the global activities at halt. In such a situation, people while staying at home...     

Analytical solutions of contaminant transport in homogeneous and isotropic aquifer in three-dimensional groundwater flow

Environmental Science and Pollution Research - Modeling three-dimensional contaminant transport released from arbitrary shape source geometries is useful in hydrological and environmental sciences....