Using vertebrate environmental DNA from seawater in biomonitoring of marine habitats

Conservation and management of marine biodiversity depends on biomonitoring of marine habitats, but current approaches are resource-intensive and require different approaches for different organisms. Environmental DNA (eDNA) extracted from water samples is an efficient and versatile approach to detecting aquatic animals. In the ocean, eDNA composition reflects local fauna at fine spatial scales, but little is known about the effectiveness of eDNA-based monitoring of marine communities at larger scales. We investigated the potential of eDNA to characterize and distinguish marine communities at large spatial scales by comparing vertebrate species composition among marine habitats in Qatar, the Arabian Gulf (also known as the Persian Gulf), based on eDNA metabarcoding of seawater samples. We conducted species accumulation analyses to estimate how much of the vertebrate diversity we detected. We obtained eDNA sequences from a diverse assemblage of marine vertebrates, spanning 191 taxa in 73 families. These included rare and endangered species and covered 36% of the bony fish genera previously recorded in the Gulf. Sites of similar habitat type were also similar in eDNA composition. The species accumulation analyses showed that the number of sample replicates was insufficient for some sampling sites but suggested that a few hundred eDNA samples could potentially capture >90% of the marine vertebrate diversity in the study area. Our results confirm that seawater samples contain habitat-characteristic molecular signatures and that eDNA monitoring can efficiently cover vertebrate diversity at scales relevant to national and regional conservation and management.     

Characteristics that make trophy hunting of giant pandas inconceivable

In November 1928, Theodore Jr. and Kermit Roosevelt led an expedition to China with the expressed purpose of being the first Westerners to kill the giant panda (Ailuropoda melanoleuca). The expedition lasted 8 months and resulted in the brothers shooting a giant panda in the mountains of Sichuan Province. Given the concurrent attention in the popular press describing this celebrated expedition, the giant panda was poised to be trophy hunted much like other large mammals around the world. Today, however, the killing of giant pandas, even for the generation of conservation revenue, is unthinkable for reasons related to the species itself and the context, in time and space, in which the species was popularized in the West. We found that the giant panda's status as a conservation symbol, exceptional charisma and gentle disposition, rarity, value as a nonconsumptive ecotourism attraction, and endemism are integral to the explanation of why the species is not trophy hunted. We compared these intrinsic and extrinsic characteristics with 20 of the most common trophy-hunted mammals to determine whether the principles applying to giant pandas are generalizable to other species. Although certain characteristics of the 20 trophy-hunted mammals aligned with the giant panda, many did not. Charisma, economic value, and endemism, in particular, were comparatively unique to the giant panda. Our analysis suggests that, at present, exceptional characteristics may be necessary for certain mammals to be excepted from trophy hunting. However, because discourse relating to the role of trophy hunting in supporting conservation outcomes is dynamic in both science and society, we suspect these valuations will also change in future.     

Applying the zoo model to conservation of threatened exceptional plant species

Maintaining a living plant collection is the most common method of ex situ conservation for plant species that cannot be seed banked (i.e., exceptional species). Viability of living collections, and their value for future conservation efforts, can be limited without coordinated efforts to track and manage individuals across institutions. Using a pedigree-focused approach, the zoological community has established an inter-institutional infrastructure to support long-term viability of captive animal populations. We assessed the ability of this coordinated metacollection infrastructure to support the conservation of 4 plant species curated in living collections at multiple botanic gardens around the world. Limitations in current practices include the inability to compile, share, and analyze plant collections data at the individual level, as well as difficulty in tracking original provenance of ex situ material. The coordinated metacollection framework used by zoos can be adopted by the botanical community to improve conservation outcomes by minimizing the loss of genetic diversity in collections. We suggest actions to improve ex situ conservation of exceptional plant species, including developing a central database to aggregate data and track unique individuals of priority threatened species among institutions and adapting a pedigree-based population management tool that incorporates life-history aspects unique to plants. If approached collaboratively across regional, national, and global scales, these actions could transform ex situ conservation of threatened plant species.     

Experimental investigation of a line plume in a filling box

Environmental Fluid Mechanics - A series of experiments were conducted to quantify the dynamics of a filling box driven by a line plume that spans the full width of the enclosure. Three...     

Circumpolar status of Arctic ptarmigan: Population dynamics and trends

Rock ptarmigan (Lagopus muta) and willow ptarmigan (L. lagopus) are Arctic birds with a circumpolar distribution but there is limited knowledge about their status and trends across their circumpolar distribution. Here, we compiled information from 90 ptarmigan study sites from 7 Arctic countries, where almost half of the sites are still monitored. Rock ptarmigan showed an overall negative trend on Iceland and Greenland, while Svalbard and Newfoundland had positive trends, and no significant trends in Alaska. For willow ptarmigan, there was a negative trend in mid-Sweden and eastern Russia, while northern Fennoscandia, North America and Newfoundland had no significant trends. Both species displayed some periods with population cycles (short 3–6 years and long 9–12 years), but cyclicity changed through time for both species. We propose that simple, cost-efficient systematic surveys that capture the main feature of ptarmigan population dynamics can form the basis for citizen science efforts in order to fill knowledge gaps for the many regions that lack systematic ptarmigan monitoring programs.

     

Hurricane Harvey and Greater Houston households: comparing pre-event preparedness with post-event health effects,event exposures,and recovery

Most disaster studies rely on convenience sampling and ‘after-only’ designs to assess impacts. This paper, focusing on Hurricane Harvey (2017) and leveraging a pre-/post-event sample of Greater Houston households (n=71) in the United States, establishes baselines for disaster preparedness and home structure flood hazard mitigation, explores household-level ramifications, and examines how preparedness and mitigation relate to health effects, event exposures, and recovery. Between 70 and 80 per cent of participants instituted preparedness measures. Mitigation actions varied: six per cent had interior drainage systems and 83 per cent had elevated indoor heating/cooling components. Sixty per cent reported home damage. One-half highlighted allergies and two-thirds indicated some level of post-traumatic stress (PTS). Three-quarters worried about family members/friends. The results of generalised linear models revealed that greater pre- event mitigation was associated with fewer physical health problems and adverse experiences, lower PTS, and faster recovery. The study design exposed the broad benefits of home structure flood hazard mitigation for households after Harvey.     

Fine-resolution mapping of wildfire fuel types for Canada: Fuzzy logic modeling for an Alberta pilot area

The Canadian Forest Fire Danger Rating System (CFFDRS) is used daily across Canada for evaluating forest fire danger. Fuel-type information is one of the inputs required by the models used in the CFFDRS. In this project, three fuel-type maps with a 25 m resolution were produced for a pilot study area located in Alberta using land cover only; land cover and biomass; and, land cover, biomass and leaf area index data derived from satellite imagery. The relationships between inputs and fuel types were determined mainly by the opinions of forest fire scientists and incorporated into a computer program using fuzzy set methodology. Not all the CFFDRS fuel types could be distinguished using these inputs; three of the coniferous types had to be grouped into one common fuel type. Overall accuracy was between 74 and 83% based on ground-truth comparisons. The most accurate map resulted from land cover and biomass data. Detailed accuracy assessment indicated that the overall accuracy increased up to 86% if ambiguous fuel type identification was considered. No combination of inputs was able to define a fuel type with absolute certainty, which is a reflection of differing expert opinions and the small number of inputs used to produce the maps.     

A Simulation Study to Assess the Sensitivity of a Forest Health Monitoring Network to Outbreaks of Defoliating Insects

The sensitivity of the United States Forest Health Monitoring network to outbreaks of defoliating insects was examined by means of a simulation study. A model constructed specifically for the study was used to generate a wide variety of defoliation patterns in forested landscapes. Forest configuration was that of Minnesota, USA, as expressed by the GAP land cover classification. Combinations of model parameters were based on a Latin Hypercube sample. The relationship between the average number of plots defoliated and outbreak characteristics was then examined via multiple regression. Both theoretical and model results pointed to a strong, linear relationship between the average number of plots defoliated and outbreak size. Model results provided additional insight, suggesting a significant relationship between the average number of plots defoliated and other outbreak characteristics after outbreak size was taken into account.     

Modelling Phosphorus Retention in Lakes and Reservoirs

Steady-state models for the prediction of P retention coefficient (R) in lakes were evaluated using data from 93 natural lakes and 119 reservoirs situated in the temperate zone. Most of the already existing models predicted R relatively successfully in lakes while it was seriously under-estimated in reservoirs. A statistical analysis indicated the main causes of differences in R between lakes and reservoirs: (a) distinct relationships between P sedimentation coefficient, depth, and water residence time; (b) existence of significant inflow–outflow P concentration gradients in reservoirs. Two new models of different complexity were developed for estimating R in reservoirs: , where τ is water residence time (year), was derived from the Vollenweider/Larsen and Mercier model by adding a calibrated parameter accounting for spatial P non-homogeneity in the water body, and is applicable for reservoirs but not lakes, and , where [P_in] is volume-weighted P concentration in all inputs to the water body (μg l⁻¹), was obtained by re-calibrating the OECD general equation, and is generally applicable for both lakes and reservoirs. These optimised models yield unbiased estimates over a large range of reservoir types.     

Implications of changing national policies on land use in the Chittagong Hill Tracts of Bangladesh

Land use in the Chittagong Hill Tracts (CHT) of Bangladesh had undergone changes over the past several centuries. The landscape, which was mostly covered with forest with interspersed shifting cultivation plots until the beginning of the colonial period, has gradually changed into a landscape with a blend of land uses. Overall, the forest area has gradually declined, while the area under shifting cultivation and sedentary agriculture has expanded. The process of the change was multi-directional. National forestry, land use, land taxation, population migration policies, and development activities, such as construction of a hydroelectric dam and roads, played an important role in this process. Shifting cultivation had inflicted little damage on the forest until the beginning of the colonial period. The pace of deforestation accelerated with the nationalization of forests which abolished tribal people's customary use and management rights to the forest, and allowed large-scale commercial logging both legally and illegally. The pace was further intensified by the policy encouraging population migration to CHT and construction of a reservoir on the Karnafuli River. Efforts were made to replace shifting cultivation with more productive types of sedentary agriculture. However, much change could not take place in the absence of secure land rights, supportive trade policies, and the required support services and facilities, including infrastructure. Locationally suitable land use evolved in areas where transportation facilities were available and farmers were granted land title with the necessary extension services and credit facilities. These findings have important policy implications for the promotion of environmentally and economically sound land use in CHT.