Improving nitrogen removal in two modified decentralized wastewater systems

Efficient nutrient removal in decentralized wastewater treatment systems is a challenging task. To improve the removal of organic matter and nitrogen from wastewater, two types of bioreactors using membrane-aerated biofilm reactor (MABR) and microbial fuel cell (MFC) techniques were evaluated. During more than 250 days of continuous-flow reactor operation, both reactors showed consistently high chemical oxygen demand removal (>86%). At an influent ammonium-nitrogen (NH4(+)-N) concentration of 30 mg N/L, the average effluent NH4(+)-N concentrations were 6.2 and 0.5 mg N/L for the MABR and MFC reactor, respectively, while the effluent nitrate-nitrogen (NO3(-)-N) concentrations were 5.4 mg/ L in the MABR and 19.2 mg/L in the MFC-based reactor. The overall total inorganic nitrogen removal efficiencies were 64% and 36% for the MABR and MFC reactor, respectively. At the measured dissolved oxygen concentrations of 5.2 and 0.23 mg/L in the aerobic/anoxic zone of the MFC and MABR, respectively, a specific oxygen uptake rate of 0.1 g O2/g VSS-d, resulting from ammonia oxidation, was detected in the settled sludge of the MFC, while no nitrifying activity of the sludge from the MABR was detected. Molecular microbial analysis demonstrated a link between the bacterial community structure and nitrifying activity. The relatively high abundance of Nitrosomonas europaea was associated with its detectable nitrification activity in the settled sludge of the MFC. The results suggest that MABR and MFC techniques have the potential to improve organic and nitrogen removal in decentralized wastewater systems.     

Occurrence of antimicrobials in animal manure-amended soils around the breeding farms: The case of the Saigon River (southern Vietnam)

The excessive use of antimicrobials in animal rearing and the associated environmental hazards have become a pressing issue. Animal agriculture is often viewed as a significant contributor to environmental degradation due to the residues of antimicrobials. It is a common practice to use livestock waste as a soil enhancer in farming. Despite some research into antimicrobials, there is room for more comprehensive data regarding these pollutants in animal farming environments. A handful of earlier studies have identified antimicrobials in animal waste. This research undertook the task of examining and evaluating soils amended with animal waste (from chickens, cows, and pigs) for the presence of seven specific antimicrobials. The antimicrobials under scrutiny included trimethoprim (TRI), ormethoprim (ORM), ofloxacin (OFL), norfloxacin (NOR), tetracycline (TET), chlortetracycline (CTE), and tylosin (TLS). Soil samples were collected from areas surrounding breeding farms located upstream of the Sai Gon River. These samples were then subjected to laboratory analysis, which involved solid-phase extraction using ultrasonic waves and the application of high-performance liquid chromatography-tandem mass spectrometry (LCMS/MS) to identify the antimicrobials. TRI, which had the highest average concentration (2.603–91.304 μg/kg), and OFL, with the second highest average concentration (1.815–15.832 μg/kg), were detected in all soil samples amended with manure. CTE, with the third highest average concentration, was found in soils amended with cow and pig waste (1.625–15.486 μg/kg). ORM and TE, with lower average concentrations (0.595–1.318 μ and 11.537–13.569 μg/kg, respectively), were only detected in soils amended with chicken waste, while NOR was only found in soils amended with cow waste. These findings indicate that the use of antimicrobials in animal farming can negatively impact the soil ecosystem. Consequently, these results can contribute to the creation of guidelines for monitoring antimicrobial residues in agricultural ecosystems.     

In vivo assessment of plant extracts for control of plant diseases: A sesquiterpene ketolactone isolated from Curcuma zedoaria suppresses wheat leaf rust

As an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. In this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Puccinia triticina, and Blumeria graminis f. sp. hordei. Of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plant diseases. In particular, a methanol extract of Curcuma zedoaria rhizomes exhibited strong activity against wheat leaf rust caused by P. triticina. When the C. zedoaria methanol extracts were partitioned with various solvents, the layers of n-hexane, methylene chloride, and ethyl acetate showed disease control values of 100, 80, and 43%, respectively, against wheat leaf rust. From the C. zedoaria rhizome extracts, an antifungal substance was isolated and identified as a sesquiterpene ketolactone based on the mass and nuclear magnetic resonance spectral data. The active compound controlled the development of rice sheath blight, wheat leaf rust, and tomato late blight. Considering the in vivo antifungal activities of the sesquiterpene ketolactone and the C. zedoaria extracts, these results suggest that C. zedoaria can be used as a potent fungicide in organic agriculture.     

A conductive polypyrrole based ammonium ion selective electrode

In view of the development of miniaturized sensor arrays, a solid-contact ammonium ion selective electrode has been investigated. A conductive polypyrrole film was electrochemicallydeposited on a glassy carbon surface and used as an internal solid contact layer between the sensing membrane and solid electrode surface. A systematic evaluation of the importantparameters affecting the electromotive force (emf) response ispresented. The performances of this solid-contact sensor were verified using a batch-mode measurement setup and a wall-jet flow cell system. The designed sensor exhibited excellent selectivity for the primary ion and a linear response over the pNH₄ ⁺ range 1–5 with a slope of 56.3 mV decade^-1. The sensor has a fast response and is relativelyrobustness, and was also used to determine ammonium concentrations in natural waters, with promising results.     

How honeybees perceive communication dances,studied by means of a mechanical model

Summary A mechanical model of a dancing honeybee was used to investigate the role of various components of the wagging dance in the transfer of information to follower bees. The model simulates the dance, carries a scent, and has an acoustic near-field similar to that of live dancers. The movements of the model are controlled by a computer, and selected components of the dance can be manipulated independently of others. The number of bees approaching scented baits at various distances and directions from the hive was observed, both during simulated normal dances and dances in which different components provided potentially conflicting information about the location of the food. The results indicate that the wagging run is the master component of the dance. The figure-of-eight dance path does not seem to convey information. Both sound and wagging must be present in the dance, but no specific roles were found for these components. Both sound and wagging convey information about distance and direction, and they appear to be largely redundant. Offprint requests to: A. Michelsen     

Community concerns about landfills: A case study of Hanoi,Vietnam

This paper examines the nature of community concerns about a landfill in Hanoi, Vietnam and compares these concerns to those found in studies of North American landfills. Based on a survey of 174 households living near Hanoi's landfill, it was found that concerns were uniformly high within the study area. Although the community initially believed the proponent's claims that the landfill would not pollute the environment, their faith in those claims disappeared once the landfill opened and began to produce numerous negative impacts on the community. Residents called for a variety of technical, management and economic measures to mitigate these impacts.     

An approach for particle sinking velocity measurements in the 3–400?μm size range and considerations on the effect of temperature on sinking rates

The flux of organic particles below the mixed layer is one major pathway of carbon from the surface into the deep ocean. The magnitude of this export flux depends on two major processes—remineralization rates and sinking velocities. Here, we present an efficient method to measure sinking velocities of particles in the size range from approximately 3–400?μm by means of video microscopy (FlowCAM^?). The method allows rapid measurement and automated analysis of mixed samples and was tested with polystyrene beads, different phytoplankton species, and sediment trap material. Sinking velocities of polystyrene beads were close to theoretical values calculated from Stokes’ Law. Sinking velocities of the investigated phytoplankton species were in reasonable agreement with published literature values and sinking velocities of material collected in sediment trap increased with particle size. Temperature had a strong effect on sinking velocities due to its influence on seawater viscosity and density. An increase in 9?°C led to a measured increase in sinking velocities of ~40?%. According to this temperature effect, an average temperature increase in 2?°C as projected for the sea surface by the end of this century could increase sinking velocities by about 6?% which might have feedbacks on carbon export into the deep ocean.     

Developments in forward osmosis and membrane distillation for desalination of waters

Membrane technology has become a common separation technology over the past decennia. Membranes are used more and more for the production of drinkable water from groundwater, surface water and wastewater. Membranes are now competitive versus conventional techniques. Desalination is predominantly used to eradicate the problem of water scarcity. The sustainability of all desalination processes depends mainly on the reduction of energy costs (production cost) and the increase in water recovery. Forward osmosis and membrane distillation are emerging technologies for sustainable desalination. Here we review membrane processes of forward osmosis and membrane distillation and the advancements in membrane material and modules. We also discuss the capability of membrane distillation in treating highly concentrated aqueous solutions derived from other desalination processes. Furthermore, the advancements in fabrication of high-performance membrane is reviewed and the performance of different membranes and optimization of membrane distillation process are summarized.     

Working in small enterprises – Is there a special risk?

Ownership structure is an important factor. For independent enterprises, the ergonomic, physical and chemical work environment is more hazardous in small enterprises than in large ones. For enterprises that are part of a larger organization, the work environment tends to be more hazardous in large enterprises when controlling for the same factors. For the psychosocial factors, the trend is different – better in small than in large enterprises independent of ownership. In all enterprises, both public and private, the quality of health and safety management systems and workplace assessment is remarkably higher in large than in small enterprises.These differences in work environment for small and large enterprises have been studied in the ‘Danish Work Environment Cohort Study’ and ‘Surveillance of health and safety activities in enterprises’. These datasets are linked together to a reliable database with data on work environment and enterprises including size, ownership structure, and health and safety management.