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Vogel JR Stoeckel DM Lamendella R Zelt RB Santo Domingo JW Walker SR Oerther DB 《Journal of environmental quality》2007,36(3):718-729
Given known limitations of current microbial source-tracking (MST) tools, emphasis on small, simple study areas may enhance interpretations of fecal contamination sources in streams. In this study, three MST tools-Escherichia coli repetitive element polymerase chain reaction (rep-PCR), coliphage typing, and Bacteroidales 16S rDNA host-associated markers-were evaluated in a selected reach of Plum Creek in south-central Nebraska. Water-quality samples were collected from six sites. One reach was selected for MST evaluation based on observed patterns of E. coli contamination. Despite high E. coli concentrations, coliphages were detected only once among water samples, precluding their use as a MST tool in this setting. Rep-PCR classification of E. coli isolates from both water and sediment samples supported the hypothesis that cattle and wildlife were dominant sources of fecal contamination, with minor contributions by horses and humans. Conversely, neither ruminant nor human sources were detected by Bacteroidales markers in most water samples. In bed sediment, ruminant- and human-associated Bacteroidales markers were detected throughout the interval from 0 to 0.3 m, with detections independent of E. coli concentrations in the sediment. Although results by E. coli-based and Bacteroidales-based MST methods led to similar interpretations, detection of Bacteroidales markers in sediment more commonly than in water indicates that different tools to track fecal contamination (in this case, tools based on Bacteroidales DNA and E. coli isolates) may have varying relevance to the more specific goal of tracking the sources of E. coli in watersheds. This is the first report of simultaneous, toolbox approach application of a library-based and marker-based MST analyses to flowing surface water. 相似文献
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Lisa G. Shaffer Justine Coppinger S. Annie Morton Sarah Alliman Jessica Burleson Ryan Traylor Cathryn Walker Steve Byerly Allen N. Lamb Roger Schultz J. Britt Ravnan Catherine D. Kashork Beth S. Torchia Scott Sulpizio Kyle Sundin Mack Schermer Karl Adler Stephanie Dallaire Blake C. Ballif 《黑龙江环境通报》2011,31(8):778-787
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Barnacles: Possible indicators of zinc pollution? 总被引:1,自引:0,他引:1
Barnacles [Balanus balanoides (L.), Elminius modestus Darwin, Lepas anatifera (L.)] from several different sites were found to accumulate the heavy metal zinc. The majority of the zinc was deposited in the tissues associated with the gut, and the level of zinc in soft body tissue generally reflected well the level of zinc in the immediate sea-water environment. The zinc accumulated in the gut tissues was in the form of discrete granules, mainly within the parenchyma cells which surround the gut. These granules probably exist as an insoluble zinc salt. A comparison of the zinc level in barnacles with those recorded for some other marine organisms indicates the possible usefulness of barnacles as indicators of zinc pollution. 相似文献