In‐well sediment incubators to evaluate microbial community stability and dynamics following bioimmobilization of uranium

An in‐well sediment incubator (ISI) was developed to investigate the stability and dynamics of sediment‐associated microbial communities to prevailing subsurface oxidizing or reducing conditions. Herein we describe the use of these devices at the Old Rifle Uranium Mill Tailings Remedial Action (UMTRA) site. During a seven‐month period in which oxidized Rifle Aquifer background sediment (RABS) were deployed in previously biostimulated wells under iron‐reducing conditions, cell densities of known iron‐reducing bacteria, including Geobacteraceae, increased significantly, showing the microbial community response to local subsurface conditions. Phospholipid fatty acid (PLFA) profiles of RABS following in situ deployment were strikingly similar to those of adjacent sediment cores, suggesting ISI results could be extrapolated to the native material of the test plots. Results for ISI deployment with laboratory‐reduced sediments showed only slight changes in community composition and pointed toward the ability of the ISI to monitor microbial community stability and response to subsurface conditions. © 2009 Wiley Periodicals, Inc.     

Studies on Soil Microarthropods Associated With Mechanic Workshops in Benin City

This study assessed the abundance of soil microarthropods in the soil in proximity to three auto mechanic workshops in Benin City, Nigeria, to determine the effect of petroleum hydrocarbon wastes on these organisms. A Berlese funnel was used to collect the microarthropods from soil samples collected from one mechanic workshop located in each of the three local government areas within Benin City: Oredo (site 1), Egor (site 2), and Ikpoba‐Okha (site 3). A Control Site soil sample was also collected from an area where no mechanic workshops were present. Inspection of the soil samples revealed a total number of 198 soil microarthropods belonging to two classes (Insecta and Acarina), eight families, and 10 species: Achegozetes longisetosus, Dermatophagoides pteronyssinus, Eremaeus columbianus, Eremaeus oblongus, Euzetes globules, Isotomurus palustris, Phlebotomus duboscqi, Solenopsis invicta, Hydrachna magnicutata, and an unidentified species were recorded. S. invicta was the most abundant, whereas the least abundant were E. columbianus, E. oblongus, and E. globules. Although there were no significant differences (p > 0.05) in mean temperature, pH, or soil moisture content across the study sites, there was a significant difference (p < 0.01) in the mean total hydrocarbon content (THC) measurements for sites 1, 2, 3, and the Control Site (48.62 ± 6.19, 51.70 ± 4.86, 52.69 ± 4.58, and 0.05 ± 0.01, respectively). Furthermore, soil microarthropod abundance and physiochemical parameters were evenly distributed from month to month. We observed a negative correlation of THC with soil microarthropod abundance across sites, although the microarthropods abundance was independent of the THC concentrations (expressed in parts per million [ppm]). In addition, although our analysis revealed no significant difference (p > 0.05) in microarthropod abundance across sites 1 through 3, abundance varied significantly (p < 0.05) between sites 1 through 3 collectively when compared with the abundance observed in samples from the Control Site. The implication of this survey is that the indiscriminate dumping of spent oils onto the soil has adverse effects on both the environment and the abundance of soil microarthropods.     

Atmospheric oxidation capacity in the summer of Houston 2006: Comparison with summer measurements in other metropolitan studies

Both similarities and differences in summertime atmospheric photochemical oxidation appear in the comparison of four field studies: TEXAQS2000 (Houston, 2000), NYC2001 (New York City, 2001), MCMA2003 (Mexico City, 2003), and TRAMP2006 (Houston, 2006). The compared photochemical indicators are OH and HO₂ abundances, OH reactivity (the inverse of the OH lifetime), HO_x budget, OH chain length (ratio of OH cycling to OH loss), calculated ozone production, and ozone sensitivity. In terms of photochemical activity, Houston is much more like Mexico City than New York City. These relationships result from the ratio of volatile organic compounds (VOCs) to nitrogen oxides (NO_x), which are comparable in Houston and Mexico City, but much lower in New York City. Compared to New York City, Houston and Mexico City also have higher levels of OH and HO₂, longer OH chain lengths, a smaller contribution of reactions with NO_x to the OH reactivity, and NO_x-sensitivity for ozone production during the day. In all four studies, the photolysis of nitrous acid (HONO) and formaldehyde (HCHO) are significant, if not dominant, HO_x sources. A problematic result in all four studies is the greater OH production than OH loss during morning rush hour, even though OH production and loss are expected to always be in balance because of the short OH lifetime. The cause of this discrepancy is not understood, but may be related to the under-predicted HO₂ in high NO_x conditions, which could have implications for ozone production. Three photochemical indicators show particularly high photochemical activity in Houston during the TRAMP2006 study: the long portion of the day for which ozone production was NO_x-sensitive, the calculated ozone production rate that was second only to Mexico City's, and the OH chain length that was twice that of any other location. These results on photochemical activity provide additional support for regulatory actions to reduce reactive VOCs in Houston in order to reduce ozone and other pollutants.     

Macrofaunal recolonisation following the intertidal placement of fine-grained dredged material

Dredged material is increasingly being regarded as a potential resource, and one of its many uses is to create and/or improve intertidal habitats (i.e. beneficial use). However, uncertainties over the longer-term environmental consequences of such schemes have, to date, limited the practice to small-scale applications in UK waters. This paper studies the macrofaunal recolonisation of fine-grained dredged material recharged concurrently at four adjacent recharge areas along the south-east coast of the UK in order to facilitate predictions regarding the recolonisation of comparable schemes and, thereby, to promote effective environmental management. During the 2-year study period, the four recharge areas were distinctly different in terms of their environmental characteristics, primarily wave exposure and bed level. These conditions resulted in different macrofaunal recolonisation rates and community structures. While the low-level, wave-sheltered area experienced rapid recolonisation, the process was delayed until 12 months post-recharge at the relatively wave-exposed areas. Bed level differences resulted in distinctly different communities in wave-sheltered areas but not under wave-exposed conditions. While we are unable to separate the effects of individual environmental variables on recolonisation, these results provide general conclusions as to the importance of environmental conditions on resulting macrofaunal communities.     

Vasculature of the hive: heat dissipation in the honey bee (Apis mellifera) hive

Eusocial insects are distinguished by their elaborate cooperative behavior and are sometimes defined as superorganisms. As a nest-bound superorganism, individuals work together to maintain favorable nest conditions. Residing in temperate environments, honey bees (Apis mellifera) work especially hard to maintain brood comb temperature between 32 and 36 °C. Heat shielding is a social homeostatic mechanism employed to combat local heat stress. Workers press the ventral side of their bodies against heated surfaces, absorb heat, and thus protect developing brood. While the absorption of heat has been characterized, the dissipation of absorbed heat has not. Our study characterized both how effectively worker bees absorb heat during heat shielding, and where worker bees dissipate absorbed heat. Hives were experimentally heated for 15 min during which internal temperatures and heat shielder counts were taken. Once the heat source was removed, hives were photographed with a thermal imaging camera for 15 min. Thermal images allowed for spatial tracking of heat flow as cooling occurred. Data indicate that honey bee workers collectively minimize heat gain during heating and accelerate heat loss during cooling. Thermal images show that heated areas temporarily increase in size in all directions and then rapidly decrease to safe levels (<37 °C). As such, heat shielding is reminiscent of bioheat removal via the cardiovascular system of mammals.     

"It's not that simple": A collaborative comparison of sea ice environments, their uses, observed changes, and adaptations in Barrow, Alaska, USA, and Clyde River, Nunavut, Canada

The Arctic environment, including sea ice, is changing. The impacts of these changes to Inuit and I?upiat ways of life vary from place to place, yet there are common themes as well. The study reported here involved an exchange of hunters, Elders, and others from Barrow, Alaska, USA, and Clyde River, Nunavut, Canada, as members of a larger research team that also included visiting scientists. Although the physical environments of Barrow and Clyde River are strikingly different, the uses of the marine environment by residents, including sea ice, had many common elements. In both locations, too, extensive changes have been observed in recent years, forcing local residents to respond in a variety of ways. Although generally in agreement or complementary to one another, scientific and indigenous knowledge of sea ice often reflect different perspectives and emphases. Making generalizations about impacts and responses is challenging and should therefore be approached with caution. Technology provides some potential assistance in adapting to changing sea ice, but by itself, it is insufficient and can sometimes have undesirable consequences. Reliable knowledge that can be applied under changing conditions is essential. Collaborative research and firsthand experience are critical to generating such new knowledge.