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281.
Green sea urchins, Strongylocentrotus droebachiensis OF Müller, collected off the coast of New Hampshire, USA, in late February 1995, were brought into the laboratory and fed
an artificial diet ad libitum, and subjected to a photoperiod advanced by 4 months. During this study, temperatures and salinities
for experimental urchins mirrored those recorded at the collection site. We examined the effects of changes in feeding regime
and photoperiod on gametogenesis and compared the experimental urchins with those from the source population. During the 7-month
period, experimental urchins showed no detectable changes in mean test height or diameter. Experimental urchins had a significantly
higher gonad index (GI) in March, April and May (18 ± 6%) compared with field urchins in March (11 ± 3%). Subsequently, experimental
urchins had a mean monthly GI of 25 to 30%, while the mean GI for field urchins was 11 to 13%. Gonial cell mitosis and gametogenesis
occurred earlier in experimental male and female urchins compared with field urchins. Stereological and histological observations
and stage–frequency data showed that the ovaries of experimental urchins were large because of the accelerated development
of nutritive phagocytes, the volume fraction (V
v) of which was 89 to 90% of the gonad, while new vitellogenic primary oocytes occupied <1% V
v. In males, stereological and histological observations and stage–frequency data suggested a mobilization of materials from
the nutritive phagocytes beginning between June and August, i.e. earlier than in females, and, by September, new gametes occupied
a V
v of 49 ± 3% of the testes. Oocyte size–frequency distributions demonstrated that most primary oocytes were <80 μm in diameter
between March and September, suggesting that cold temperatures may be needed for completion of vitellogenesis. We describe
changes in the two principal cell types in the germinal epithelium of urchin gonads and indicate how knowledge of their population
dynamics may be useful in aquaculture applications.
Received: 21 February 1997 / Accepted: 3 June 1998 相似文献
282.
Although scyphomedusae have received increased attention in recent years as important predators in coastal and estuarine
environments, the factors affecting zooplankton prey vulnerability to these jellyfish remain poorly understood. Current models
predicting feeding patterns of cruising entangling predators, such as Chrysaora quinquecirrha (Desor, 1948), fail to account for the selection of fast-escaping prey such as copepods. Nevertheless, our analysis of gastric
contents of field-collected medusae showed that this scyphomedusa fed selectively on the calanoid copepod Acartia tonsa (Dana, 1846) and preferentially ingested adult over copepodite stages. We measured feeding rates in a planktonkreisel while
simultaneously videotaping predator–prey interactions. C. quinquecirrha consumed adult A. tonsa ten times faster than copepodites. Differences in prey behavior, in the form of predator–prey encounter rates or post-encounter
escape responses, could not account for the elevated feeding rates on adults. Prey size, however, had a dramatic impact on
the vulnerability of copepods. In experiments using heat-killed prey, feeding rates on adults (1.5 times longer than copepodites)
were 11 times higher than on copepodites. In comparison, medusae ingested heat-killed prey at only two to three times the
rate of live prey. These results suggest that during scyphomedusan–copepod interactions, prey escape ability is important,
but ultimately small size is a more effective refuge from predation.
Received: 26 September 1997 / Accepted: 20 May 1998 相似文献
283.
We examined larval response to a range of sharp haloclines and determined the effect of dietary conditioning on that response
in the sea urchins Echinometra lucunter and Arbacia punctulata. We reared larvae in the laboratory under a high or low concentration of either single (Isochrysis galbana) or mixed (Isochrysis galbana, Dunaliella tertiolecta, Thalassiosira weissflogii) microalgal species. For both species of sea urchins, rate of larval development was faster and age-specific larval length
and width were greater in high-ration than low-ration diets. We examined the distribution of two- and four-arm larvae of E. lucunter from each diet treatment and of four-arm larvae of A. punctulata from the high-ration diets in cylinders with experimentally constructed haloclines. In three of the halocline treatments,
the salinity of the bottom layer was 33‰ and that of the top layer was 21, 24 or 27‰ (21/33, 24/33 and 27/33) and in a fourth
one, the salinities of the bottom and top layer were 30 and 21‰, respectively (21/30). The position of larvae in the cylinders
varied with the steepness of the halocline and with dietary conditioning for both sea urchin species and all developmental
stages tested. Significantly more larvae crossed the haloclines into water of 24 and 27‰ salinity than into water of 21‰ salinity.
We observed an effect of diet on the position of larvae in the cylinders, and that effect varied among halocline treatments
for both species. The proportion of larvae of E.lucunter that crossed the halocline was greater in low- than high-ration diets in the 24/33 and 27/33 treatments. Position of four-arm
larvae in the cylinders also varied with food quality in high-ration diets: for E.lucunter in the 24/33 treatments, and for A.
punctulata in the 21/30 treatments, more larvae from the single- than from the mixed-species diets were present above the halocline.
Salinity in the adult habitat during most of the active reproductive period ranged from 15 to 40‰. We showed that larvae can
respond to gradients in salinity, and therefore can remain within a water mass of higher salinity overlying the adult habitat.
However, survival of poorly fed larvae may be increased if they are introduced into a new water mass and carried away from
a nutritionally poor environment.
Received: 9 July 1997 / Accepted: 12 January 1998 相似文献
284.
Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization 总被引:1,自引:0,他引:1
F. Fontana J. Tagliavini L. Congiu M. Lanfredi M. Chicca C. Laurente R. Rossi 《Marine Biology》1998,132(3):495-501
A karyotype analysis by several staining techniques was carried out on the great sturgeon, Huso huso (Linnaeus, 1758). The karyotype (2n = 118 ± 2) was composed of 42 pairs of meta-/submetacentric chromosomes and 17 pairs of acrocentrics/microchromosomes. Constitutive
heterochromatin was mainly located at the centromeric regions of the acrocentric chromosomes. The biarmed chromosomes showed
weak C-bands. Fluorescent staining with GC-specific chromomycin A3 showed clearly recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific
4,6-diamidino-2 phenylindole. After Ag-staining, nucleolar organizer regions could be observed on the short arms of two medium-sized
submetacentrics and on two acrocentrics. Digoxigenated 28S and 5S rDNA probes, prepared from Acipenser naccarii DNA and hybridized to metaphase chromosomes, showed signals on six and two chromosomes, respectively. The telomeric sequence
(TTAGGG)
n
detected by FISH was located at both ends of each chromosome. Results are discussed in relation to karyotype organization
and evolution in sturgeons.
Received: 1 April 1998 / Accepted: 21 July 1998 相似文献
285.
Starch-gel electrophoresis of allozymes was used to differentiate the two red mullet species (Mullus barbatus L. and M. surmuletus L.) in the Mediterranean Sea and, further, to investigate the genetic stock structure of M. barbatus in the eastern Mediterranean area. Twenty putative enzyme-coding loci were examined in eight M. barbatus samples caught in the Aegean and Ionian Seas (Greece) and in the Gulf of Lion (France), and two M. surmuletus samples caught in the Aegean and Gulf of Lion. A high degree of genetic polymorphism was found in both species. Species-specific
electrophoretic patterns were found in PGI* and PGM*. Estimates of variance of allele frequencies among samples (F
ST) and 2 analyses both revealed significant differences (P < 0.05) among the M. barbatus samples. Most of the genetic variation was among samples regardless of region. The mean value of Nei's genetic distance between
the two species was 0.329. Genetic distance among M. barbatus samples was low (maximum Nei's D = 0.012), with the sample from Platania differing most from other M. barbatus samples. This is probably be due to founder effects existing at this area. These results suggest that allozyme analysis may
provide important information on the genetic structure of the red mullet to ensure sustainable management of this species.
Received: 7 May 1997 / Accepted: 13 October 1997 相似文献
286.
Jacobus C. Biesmeijer Mark G. L. van Nieuwstadt Saskia Lukács Marinus J. Sommeijer 《Behavioral ecology and sociobiology》1998,42(2):107-116
Social insect foragers have to make foraging decisions based on information that may come from two different sources: information
learned and memorised through their own experience (“internal” information) and information communicated by nest mates or
directly obtained from their environment (“external” information). The role of these sources of information in decision-making
by foragers was studied observationally and experimentally in stingless bees of the genus Melipona. Once a Melipona forager had started its food-collecting career, its decisions to initiate, continue or stop its daily collecting activity
were mainly based upon previous experience (activity on previous days, the time at which foraging was initiated the day(s)
before, and, during the day, the success of the last foraging flights) and mediated through direct interaction with the food
source (load size harvested and time to collect a load). External information provided by returning foragers advanced the
start of foraging of experienced bees. Most inexperienced bees initiated their foraging day after successful foragers had
returned to the hive. The start of foraging by other inexperienced bees was stimulated by high waste-removal activity of nest
mates. By experimentally controlling the entries of foragers (hence external information input) it was shown that very low
levels of external information input had large effect on the departure of experienced foragers. After the return of a single
successful forager, or five foragers together, the rate of forager exits increased dramatically for 15 min. Only the first
and second entry events had large effect; later entries influenced forager exit patterns only slightly. The results show that
Melipona foragers make decisions based upon their own experience and that communication stimulates these foragers if it concerns the
previously visited source. We discuss the organisation of individual foraging in Melipona and Apis mellifera and are led to the conclusion that these species behave very similarly and that an information-integration model (derived
from Fig. 1) could be a starting point for future research on social insect foraging.
Received: 16 April 1997 / Accepted after revision: 30 August 1997 相似文献
287.
Bushcricket song structure and predation by the acoustically orienting parasitoid fly Therobia leonidei (Diptera: Tachinidae: Ormiini) 总被引:2,自引:0,他引:2
Gerlind U. C. Lehmann Klaus-Gerhard Heller 《Behavioral ecology and sociobiology》1998,43(4-5):239-245
Males of most bushcricket species produce acoustic advertisement signals to attract females for mating. These signals can
also increase conspicuousness to predators. In the genus Poecilimon (Orthoptera: Phaneropterinae) males are attacked by the parasitoid fly Therobia leonidei (Diptera: Tachinidae: Ormiini) which locates males by their calls. In Greece T.leonidei parasitizes several Poecilimon species with different song structures: we examined whether host choice is related to song structure by comparing parasitism
rates in two closely related Poecilimon species. One of these species produces monosyllabic songs, the songs of the other species being polysyllabic. The tachinid
fly parasitized the polysyllabic species to a greater extent. We demonstrate in a field-experiment that this preference for
the polysyllabic species does not depend on local adaptations of the fly. The most probable explanation for the preference
of the fly for the polysyllabic singing species seems to be better detection of longer songs. This result is discussed in
the context of male song evolution.
Received: 4 November 1997 / Accepted after revision: 22 March 1998 相似文献
288.
289.