Fetal karyotype following ascertainment of fetal anomalies by ultrasound

Data pooled from contributors to a Registry for Cytogenetic Abnormalities and PKU (ReCAP) shows an unbalanced chromosome abnormality rate of 27 per cent (29 fetuses) for 107 fetuses with ultrasonically diagnosed fetal anomalies. Of the abnormal, 12 were trisomic, 6 were monosomy X and 6 were structural abnormalities, 4 were mosaics and one triploid.     

Widespread contribution of methane-cycle bacteria to the diets of lake profundal chironomid larvae

Reports of unexpectedly 13C-depleted chironomid larvae in lakes have led to an hypothesis that significant transfer of detrital organic matter to chironomid larvae may occur via methane-cycle bacteria. However, to date little is known of how such transfer might vary across species and lakes. We gathered data from 87 lakes to determine how widespread this phenomenon might be and to define boundaries for its likely magnitude. Carbon stable isotope values of chironomid larvae varied greatly between taxa. Very marked 13C-depletion was evident only in certain taxa, especially Chironomus plumosus, C. anthracinus, and C. tenuistylus, all characteristic of eutrophic or dystrophic lakes and known to be tolerant of low oxygen conditions. Furthermore, marked 13C-depletion was only found in larvae from lakes in which late-summer hypolimnetic oxygen depletion near the sediment surface was below an apparent threshold concentration of 2-4 mg O2/L. Similarly, application of a two-source mixing model suggested that methanotrophic bacteria made the greatest contribution to profundal chironomid growth (0-70% of larval carbon) when the late-summer oxygen concentration dropped below approximately 2 mg O2/L. Our study demonstrates that methane-derived carbon is an important, but often neglected, contribution to the flux of carbon through the food webs of many productive or dystrophic lakes.     

Testing the robustness of management decisions to uncertainty: Everglades restoration scenarios.

To effectively manage large natural reserves, resource managers must prepare for future contingencies while balancing the often conflicting priorities of different stakeholders. To deal with these issues, managers routinely employ models to project the response of ecosystems to different scenarios that represent alternative management plans or environmental forecasts. Scenario analysis is often used to rank such alternatives to aid the decision making process. However, model projections are subject to uncertainty in assumptions about model structure, parameter values, environmental inputs, and subcomponent interactions. We introduce an approach for testing the robustness of model-based management decisions to the uncertainty inherent in complex ecological models and their inputs. We use relative assessment to quantify the relative impacts of uncertainty on scenario ranking. To illustrate our approach we consider uncertainty in parameter values and uncertainty in input data, with specific examples drawn from the Florida Everglades restoration project. Our examples focus on two alternative 30-year hydrologic management plans that were ranked according to their overall impacts on wildlife habitat potential. We tested the assumption that varying the parameter settings and inputs of habitat index models does not change the rank order of the hydrologic plans. We compared the average projected index of habitat potential for four endemic species and two wading-bird guilds to rank the plans, accounting for variations in parameter settings and water level inputs associated with hypothetical future climates. Indices of habitat potential were based on projections from spatially explicit models that are closely tied to hydrology. For the American alligator, the rank order of the hydrologic plans was unaffected by substantial variation in model parameters. By contrast, simulated major shifts in water levels led to reversals in the ranks of the hydrologic plans in 24.1-30.6% of the projections for the wading bird guilds and several individual species. By exposing the differential effects of uncertainty, relative assessment can help resource managers assess the robustness of scenario choice in model-based policy decisions.     

Mutualism as reciprocal exploitation: African plant-ants defend foliar but not reproductive structures

The foundation of many plant-ant mutualisms is ant protection of plants from herbivores in exchange for food and/or shelter. While the role of symbiotic ants in protecting plants from stem- and leaf-feeding herbivores has been intensively studied, the relationship between ant defense and measures of plant fitness has seldom been quantified. We studied ant aggression, damage by herbivores and seed predators, and fruit production among Acacia drepanolobium trees occupied by four different acacia-ant species in an East African savanna. Levels of ant aggression in response to experimental disturbance differed strongly among the four species. All four ant species recruited more strongly to new leaf growth on host plants following disturbance, while recruitment to developing fruits was on average an order of magnitude lower. Host plants occupied by more aggressive ant species suffered significantly less vegetative damage from leaf-feeding insects, stem-boring beetles, and vertebrate browsers than host plants occupied by less aggressive ant species. However, there were no differences among fruiting host plants occupied by different ant species in levels of seed predation by bruchid seed predators. Fruit production on host trees was significantly correlated with tree stem diameter but not with the identity of resident ants. Our results demonstrate that defense of host plants may differ substantially among ant species and between vegetative and reproductive structures and that fruit production is not necessarily correlated with high levels of aggression by resident ants.     

Comparison of methods used to calculate typical threshold values for potentially toxic elements in soil

The environmental quality of land can be assessed by calculating relevant threshold values, which differentiate between concentrations of elements resulting from geogenic and diffuse anthropogenic sources and concentrations generated by point sources of elements. A simple process allowing the calculation of these typical threshold values (TTVs) was applied across a region of highly complex geology (Northern Ireland) to six elements of interest; arsenic, chromium, copper, lead, nickel and vanadium. Three methods for identifying domains (areas where a readily identifiable factor can be shown to control the concentration of an element) were used: k-means cluster analysis, boxplots and empirical cumulative distribution functions (ECDF). The ECDF method was most efficient at determining areas of both elevated and reduced concentrations and was used to identify domains in this investigation. Two statistical methods for calculating normal background concentrations (NBCs) and upper limits of geochemical baseline variation (ULBLs), currently used in conjunction with legislative regimes in the UK and Finland respectively, were applied within each domain. The NBC methodology was constructed to run within a specific legislative framework, and its use on this soil geochemical data set was influenced by the presence of skewed distributions and outliers. In contrast, the ULBL methodology was found to calculate more appropriate TTVs that were generally more conservative than the NBCs. TTVs indicate what a “typical” concentration of an element would be within a defined geographical area and should be considered alongside the risk that each of the elements pose in these areas to determine potential risk to receptors.     

Sequential element extraction of soils from abandoned farms: an investigation of the partitioning of anthropogenic element inputs from historic land use

Enhanced soil element concentrations may serve as indicators not only of modern pollution, but also of former historic and/or pre-historic human activity. However, there is little consensus over the most appropriate means of extraction for identifying chemical signatures of modern and archaeological pollution. This study addressed this question by using a 5-step sequential extraction to examine the partitioning of elements within the soil. Samples were taken from known functional areas (hearth, house, byre, arable, and grazing areas) on a 19th century abandoned croft (small farm). A hot nitric acid digest and five-stage sequential extraction method were used to examine the partitioning of elements in soil and identify the current elemental distribution of anthropogenic contamination. The results indicate that although a significant proportion of Ca tends to be bound with exchangeable and weak acid soluble fractions, in the hearth and house areas there is also a significant proportion held within the recalcitrant residue. Pb concentrations tend to be associated with organic matter, ammonium oxalate extractable fractions and the residue, whilst Zn generally has a more even partitioning between the six soil fractions. The implications of this for extraction methodology are element and soil specific. However, the presence of a significant proportion of anthropogenically significant elements (including Ca, Pb, Zn, Sr, and Ba) within the resistant residue suggests the use of only a weak acid or an exchangeable fraction extraction would result in the loss of information from contamination resulting from former human activity. Hence, a total or pseudo-total extraction method is recommended for this type of study.     

Sequential concentration of bacteria and viruses from marine waters using a dual membrane system

The ability to rapidly and effectively concentrate diverse microbes is an essential component for monitoring water quality at recreational beaches. The purpose of this study was to develop a 0.45 microm pore size dual membrane system, which can sequentially concentrate both viruses and bacteria. The top PVDF membrane was used to filter bacteria by physical straining while the bottom HA membrane retained viruses through adsorption. The recovery of this system was assessed using test organisms: enterococci and somatic coliphage. Volumes of 100 to 400 mL of unspiked and sewage-spiked beach water were filtered through both types of membranes. The PVDF membrane recovered statistically equivalent amounts of enterococci when compared to traditional membranes. All of the coliphage passed through the PVDF membrane, while 22% passed through the HA membrane. Increasing the volume from 100 to 400 mL did not significantly influence recoveries. Up to 35% of coliphage was eluted from the bottom membrane using beef extract solution. Rinsing bottom membranes with 0.5 mmol L(-1) H(2)S0(4) was found to deactivate somatic coliphage. This research demonstrates the potential of using a dual membrane adsorption system for the concentration of both bacteria and viruses from recreational beaches. A proposed bi-layer filtration system can be designed for simultaneous bacteria and virus filtration. Future experiments should focus on measurements utilizing additional bacteria and viruses.     

The perceived scenic effects of clearcutting in the White Mountains of New Hampshire, USA

The scenic effects of clearcutting have been a volatile issue for the American public for much of the past century. A better understanding of the scenic perceptions associated with the cumulative visible effects of clearcutting should contribute to better decisions about its use. This study evaluates the scenic impacts of simulated alternative clearcutting management systems for the White Mountain National Forest. Alternatives represented an unharvested view, and views with removal of 1–5% of the timber every 12 years using either scattered or concentrated clearcut units of 5, 15 and 30 acres in two viewsheds. A random sample of local citizens, four groups of opinion leaders, and US Forest Service employees evaluated these alternatives. Each factor accounts for significant differences in scenic value. All groups rated the scenic value of unharvested scenes very high. All groups find a large drop in scenic value with the introduction of even the least intense harvesting activity. All groups indicated a statistically significant decrease in visual quality as the intensity of harvesting increases. This effect continues throughout the range of harvesting intensities until the level of sustainable yield is reached. In addition, patches 12–15 acres in size were preferred to larger and smaller ones.