Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning detoxification

Nile tilapia (Oreochromis niloticus) were fed by diets supplemented with cyanobacteria containing in part the cyanotoxin microcystin-LR (MC-LR) to determine the potential impacts on detoxification. Four different diets were prepared based on a commercial diet: (1) control, (2) MC-5% (containing 5% dried Microcystis sp. biomass with 4.92 μg MC-LR g(-1) diet), (3) MC-20% (containing 20% dried Microcystis sp. biomass with 19.54 μg MC-LR g(-1) diet), and (4) Arthr-20% (containing 20% dried Arthrospira sp. biomass without MC-LR). Blood and liver samples were taken after one, 7, and 28 days and protein has been determined in plasma and liver. In the liver, impacts on detoxification were measured by glutathione-S-transferase (GST) activities and gene expression of multi drug resistance protein (MDRP). Plasma protein did not change between all four diets at any sampling time whereas liver protein was significantly elevated already after one day in Arthr-20% and after 28 days in both, MC-20% and Arthr-20%. Biochemical measurements of GST activities revealed no significant impact at any sampling time. In order to characterize the potential effect of MC-LR on MDRP, RT-qPCR method was established. However, as for GST activities no significant changes in MDRP gene expression have been observed. Thus, in summary, oral exposure of MC-LR containing cyanobacteria to Nile tilapia via feed ingestion did not impact significantly detoxification in liver concerning GST activities and MDRP expression despite biochemical composition concerning liver protein was significantly elevated by the diets containing 20% cyanobacteria biomass, regardless whether they contained MC-LR or not.     

Effects of walnut husk washing waters and their phenolic constituents on horticultural species

INTRODUCTION: The reuse of wastewaters for agricultural purposes is a common practice in many countries and is increasingly recommended by organizations that promote sustainable development. Yet, it is restricted by the potential negative impact of these materials on soil and crops. The aim of this study was therefore to evaluate the environmental impact of walnut husk washing waters (WHWW) and their organic fractions, in order to conceive their agricultural exploitation. DISCUSSION: Phytotoxicity tests and morphological investigations on representative plant species of horticultural interest indicated that WHWW and their organic fractions can elicit a concentration-dependent stimulating effect on the growth of radish, lettuce cv. cavolo Napoli with effects up to 165 %. An opposite inhibitory effect up to 70 % was observed on spinach and lettuce cv. Gentilina. Proapoptotic effects were observed by acridine orange/ethidium bromide assay in the species inhibited by WHWW treatment. High-performance liquid chromatography-mass spectrometry analysis of the WHWW revealed the presence of a main component which was extracted selectively in organic solvents and purified by preparative chromatography. Complete spectral analysis allowed identification as 4,8-dihydroxy-1-tetralone, commonly known as regiolone. Regiolone exhibited the same concentration-dependent activity on root elongation with a stimulation in the case of radish up to 135 % with respect to control. These results open perspectives in the exploitation of WHWW and the main phenolic constituent readily available by a straightforward isolation procedure as a natural fertilizer for specific crops.     

Preparation and Properties of Chitosan–PVA Fibers Produced by Wet Spinning

Chitosan fibers were prepared by wet spinning in three stages. Initially, a polymer solution of chitosan and polyvinyl alcohol (PVA) was solidified in a mixture of potassium hydroxide and ethanol. The polymers were then crosslinked with sodium tripolyphosphate (TPP) or glutaraldehyde, and finally dried in methanol or acetone. The effect of these conditions was evaluated based on scanning electron microscopy images, water-holding capacity, and swelling and mechanical properties. The miscibility of the mixture was evaluated using Fourier Transform Infrared spectroscopy and differential scanning calorimetry. The results obtained showed that chitosan fibers containing 45% (v/v) PVA and crosslinked using TPP have properties similar to those of commercial sutures prepared using other biomaterials.     

Can a key boreal Calanus copepod species now complete its life-cycle in the Arctic? Evidence and implications for Arctic food-webs

The changing Arctic environment is affecting zooplankton that support its abundant wildlife. We examined how these changes are influencing a key zooplankton species, Calanus finmarchicus, principally found in the North Atlantic but expatriated to the Arctic. Close to the ice-edge in the Fram Strait, we identified areas that, since the 1980s, are increasingly favourable to C. finmarchicus. Field-sampling revealed part of the population there to be capable of amassing enough reserves to overwinter. Early developmental stages were also present in early summer, suggesting successful local recruitment. This extension to suitable C. finmarchicus habitat is most likely facilitated by the long-term retreat of the ice-edge, allowing phytoplankton to bloom earlier and for longer and through higher temperatures increasing copepod developmental rates. The increased capacity for this species to complete its life-cycle and prosper in the Fram Strait can change community structure, with large consequences to regional food-webs.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13280-021-01667-y.     

Exploring work unit context and leader–member exchange: a multi‐level perspective

Leader–member exchange relationships have rarely been explored within the context of the work unit. Employing a sample of 285 employees of a southeastern county library system, the work unit contextual factors of work group size, work group cohesiveness, organizational climate, and leader power were examined for their relationship with LMX from the subordinate's perspective, using a within and between analysis (WABA) approach. All of the contextual variables except size were significantly related to LMX. The WABA and r_WG analyses revealed that effects occurred at both within‐ and between‐group levels, indicating individual difference effects. Thus, practitioners should emphasize enhancements to group and organizational processes as well as contributions toward relationship building in LMX dyads. Future research should expand the network of contextual variables that may have potential relevance with LMX, since this study demonstrated that the work context in which the dyad resides contributes in part to the LMX phenomenon. Additional study of the work unit size and LMX relationship is also warranted, as the current research failed to replicate previous research with a predicted negative relationship. Copyright © 2000 John Wiley & Sons, Ltd.     

Microbiological characterization and specific methanogenic activity of anaerobe sludges used in urban solid waste treatment

This study presents the microbiological characterization of the anaerobic sludge used in a two-stage anaerobic reactor for the treatment of organic fraction of urban solid waste (OFUSW). This treatment is one alternative for reducing solid waste in landfills at the same time producing a biogas (CH(4) and CO(2)) and an effluent that can be used as biofertilizer. The system was inoculated with sludge from a wastewater treatment plant (WWTP) (Río Frío Plant in Bucaramanga-Colombia) and a methanogenic anaerobic digester for the treatment of pig manure (Mesa de los Santos in Santander). Bacterial populations were evaluated by counting groups related to oxygen sensitivity, while metabolic groups were determined by most probable number (MPN) technique. Specific methanogenic activity (SMA) for acetate, formate, methanol and ethanol substrates was also determined. In the acidogenic reactor (R1), volatile fatty acids (VFA) reached values of 25,000 mg L(-1) and a concentration of CO(2) of 90%. In this reactor, the fermentative population was predominant (10(5)-10(6)MPN mL(-1)). The acetogenic population was (10(5)MPN mL(-1)) and the sulphate-reducing population was (10(4)-10(5)MPN mL(-1)). In the methanogenic reactor (R2), levels of CH(4) (70%) were higher than CO(2) (25%), whereas the VFA values were lower than 4000 mg L(-1). Substrate competition between sulphate-reducing (10(4)-10(5)MPN mL(-1)) and methanogenic bacteria (10(5)MPN mL(-1)) was not detected. From the SMA results obtained, acetoclastic (2.39 g COD-CH(4)g(-1)VSS(-1)day(-1)) and hydrogenophilic (0.94 g COD-CH(4)g(-1)VSS(-1)day(-1)) transformations as possible metabolic pathways used by methanogenic bacteria is suggested from the SMA results obtained. Methanotrix sp., Methanosarcina sp., Methanoccocus sp. and Methanobacterium sp. were identified.     

Transformation of diclofenac by the indigenous microflora of river sediments and identification of a major intermediate

Diclofenac is a non-steroidal anti-inflammatory drug, which tends to be relatively persistent in the environment. Now, a fixed-bed column bioreactor filled with sediment from the creek Münzbach (Freiberg/Saxony) under aerobic conditions showed rapid removal of diclofenac in a concentration range of 3-35 microM without previous adaptation. The conversion of higher concentrations up to 260 microM was accompanied by conspicuously decreased turnover rates indicating a toxic effect of this drug or its resulting metabolic burden on the indigenous microflora. A major metabolite occurred transiently and was identified by NMR and MS to be the p-benzoquinone imine of 5-hydroxydiclofenac. Abiotic adsorption to the biofilm was shown to determine the further fate of this reactive product of 5-hydroxydiclofenac (aut-)oxidation. The apparent lack of a degradative potential for this compound as well as the failure to detect an enrichment of diclofenac-depleting microbial activity both indicate a cometabolic nature of diclofenac transformation. 4'-Hydroxy-diclofenac, the favoured transformation product of eucaryotic diclofenac metabolism, could not be identified. The ability to convert diclofenac was shown to be widespread among biofilms from different river sediments, but measured rates obviously do not correlate with the total microbial activity. In addition, application of sediments from locations exposed to communal waste water effluents did not indicate any form of adaptation measured as an increased specific diclofenac depletion rate.     

Bioakkumulation und Deposition von Antimon, Blei und polyzyklischen aromatischen Kohlenwasserstoff-Immissionen

The combination of biomonitoring with standardized grass cultures and deposition monitoring of antimony (Sb), lead (Pb) and 12 polycyclic aromatic hydrocarbon compounds (PAH) was carried out in the conurbation of Munich, Germany in 1993 and resumed at the German-Czech border in 1995. Results from different location types in different areas and years seem to be comparable for Sb and PAH, because relevant background data fit in the same range. Biomonitoring reveals a seasonal course of PAH accumulation. Mean PAH contents are found to be significantly higher in grass cultures at locations along heavily traveled roads, compared to sites in living areas or in a rural background. The traffic related PAH bioaccumulation of both regions fits in the same range, although traffic composition is different and traffic density is ten times smaller at the German-Czech border. Correlation of Pb and Sb bioaccumulation in grass vs. deposition suggested abrasions of brake linings and tires to be one possible emission source. This might be the explanation for the mean contents of Pb and Sb in grass cultures at traffic locations in Munich exceeding those in living or rural areas. Deposition (Bergerhoff method) indicates analogous pollution dynamics in association with traffic. Pb bioaccumulation and deposition along the main roads at the German-Czech border show wider ranges compared to Munich. On the whole, Pb and Sb values at border traffic are found to be lower than those in Munich two years previously.     

Division of labor between scouts and recruits: genetic influence and mechanisms

Every recruitment system in social insects requires some individuals that serve as scouts, foragers that search independently for food sources. It is not well understood which factors influence whether an individual becomes a scout or a recruit, nor how the division of labor between the two forager groups is regulated. It is shown here for honeybees (Apis mellifera), using two different molecular techniques, that there is a genetically based difference in the probability that individuals will scout independently for food. In contrast to earlier suggestions, experimental tests showed that the age of a bee does not seem to influence its probability of becoming a scout or a recruit. Furthermore, scout bees do not search opportunistically for either pollen or nectar but, rather, individuals have preferences that are genetically based. These findings are discussed in the framework of foraging regulation by specialization in honeybees and the adaptive significance of polyandry. Received: 23 October 1997 / Accepted after revision: 10 April 1998