Bioaccumulation of glyphosate and its formulation Roundup Ultra in Lumbriculus variegatus and its effects on biotransformation and antioxidant enzymes

The bioaccumulation potential of glyphosate and the formulation Roundup Ultra, as well as possible effects on biotransformation and antioxidant enzymes in Lumbriculus variegatus were compared by four days exposure to concentrations between 0.05 and 5 mg L⁻¹ pure glyphosate and its formulation. Bioaccumulation was determined using ¹⁴C labeled glyphosate. The bioaccumulation factor (BCF) varied between 1.4 and 5.9 for the different concentrations, and was higher than estimated from log P_ow. Glyphosate and its surfactant POEA caused elevation of biotransformation enzyme soluble glutathione S-transferase at non-toxic concentrations. Membrane bound glutathione S-transferase activity was significantly elevated in Roundup Ultra exposed worms, compared to treatment with equal glyphosate concentrations, but did not significantly differ from the control. Antioxidant enzyme superoxide dismutase was significantly increased by glyphosate but in particular by Roundup Ultra exposure indicating oxidative stress. The results show that the formulation Roundup Ultra is of more ecotoxicological relevance than the glyphosate itself.     

Agricultural livelihood transition in the southern Yucatán region: diverging paths and their accompanying land changes

Land change science has demonstrated that rural livelihoods around the world both drive and reflect changing environmental regimes and political economic/structural transformations. This article explores the relationship between increasingly globalized rural livelihoods and in-place land change, assessing results from social surveys of smallholding households in the southern Yucatán region. We examine evidence for a transition in agricultural livelihood strategies as smallholders adjust to changing political economic and institutional conditions, and link these transitioning strategies to land use changes. Based on household surveys in 1997 and 2003, we comparatively assess both changes in the selection of livelihood strategies and in the land use and cover impacts of those strategies. Our results indicate that although impacts of given strategies have changed little over this period, there are increasing proportions of households pursuing two divergent adjustment paths—one of agricultural withdrawal and one of agricultural intensification and commercialization. We investigate what sociodemographic characteristics differentiate the groups of households following distinct livelihood strategies. Our findings point to the possibility of simultaneous and contradictory land change outcomes as smallholders adjust in different ways to their intensified incorporation into global economies.     

The impact of socio-economics and climate change on tropical cyclone losses in the USA

Tropical cyclones that make landfall on the coast of the USA are causing increasing economic losses. It is assumed that the increase in losses is largely due to socio-economic developments, i.e. growing wealth and greater settlement of exposed areas. However, it is also thought that the rise in losses is caused by increasing frequency of severe cyclones resulting from climate change, whether due to natural variability or as a result of human activity. The object of this paper is to investigate how sensitive the losses are to socio-economic changes and climate changes and how these factors have evolved over the last 50 years. We will then draw conclusions about the part the factors concerned play in the observed increase in losses. For analysis purposes, storm loss is depicted as a function of the value of material assets affected by the storm (the capital stock) and storm intensity. The findings show the increase in losses due to socio-economic changes to have been approximately three times greater than that due to climate-induced changes.     

Changes in the spatial patterns of human appropriation of net primary production (HANPP) in Europe 1990–2006

Understanding patterns, dynamics, and drivers of land use is crucial for improving our ability to cope with sustainability challenges. The human appropriation of net primary production (HANPP) framework provides a set of integrated socio-ecological indicators that quantify how land use alters energy flows in ecosystems via land conversions and biomass harvest. Thus, HANPP enables researchers to systematically and consistently assess the outcome of changes in land cover and land-use intensity across spatio-temporal scales. Yet, fine-scale HANPP assessments are so far missing, an information important to address site-specific ecological implications of land use. Here, we provide such an assessment for Europe at a 1-km scale for the years 1990, 2000, and 2006. The assessment was based on a consistent land-use/biomass flow dataset derived from statistical data, remote sensing maps, and a dynamic global vegetation model. We find that HANPP in Europe amounted to ~43 % of potential productivity, well above the global average of ~25 %, with little variation in the European average since 1990. HANPP was highest in Central Europe and lower in Northern and Southern Europe. At the regional level, distinct changes in land-use intensity were observed, most importantly the decline of cropland areas and yields following the breakdown of socialism in Eastern Europe and the subsequent recovery after 2000, or strong dynamics related to storm events that resulted in massive salvage loggings. In sum, however, these local dynamics cancelled each other out at the aggregate level. We conclude that this finding warrants further research into aspects of the scale-dependency of dynamics and stability of land use.     

Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries

The effectiveness of genetic bioaugmentation relies on efficient plasmid transfer between donor and recipient cells as well as the plasmid’s phenotype in the recipient cell. In the present study, the effects of varying organic carbon substrates, initial recipient-to-donor cell density ratios, and mixtures of known recipient bacterial strains on the conjugation and function of a TOL plasmid were tested in sterile soil slurry batch reactors. The presence of soil organic carbon was sufficient in ensuring TOL plasmid transconjugant occurrence (up to 2.1 ± 0.5%) for most recipient strains in soil slurry batch mating experiments. The addition of glucose had limited effects on transconjugant occurrence; however, glucose amendment increased the specific toluene degradation rates of some Enterobacteriaceae transconjugants in soil slurry. Initial cell density ratios and mixtures of recipient strains had smaller impacts on plasmid conjugation and resulting phenotype functionality. These observations suggest that genetic bioaugmentation may be improved by minimal altering of environmental conditions.     

Solid beef cattle manure application impacts on soil properties and 17β-estradiol fate in a clay loam soil

Livestock manure applied to agricultural land is one of the ways natural steroid estrogens enter soils. To examine the impact of long-term solid beef cattle (Bos Taurus) manure on soil properties and 17β-estradiol sorption and mineralization, this study utilized a soil that had received beef cattle manure over 35 years. The 17β-estradiol was strongly sorbed and sorption significantly increased (P < 0.05) with increasing soil organic carbon content (SOC) and with an increasing annual rate of beef cattle manure. The 17β-estradiol mineralization half-life was significantly negatively correlated, and the total amount of 17β-estradiol mineralized at 90 days (MAX) was significantly positively correlated with 17β-estradiol sorption. The long-term rate of manure application had no significant effect on MAX, but the addition of fresh beef cattle manure in the laboratory resulted in significantly (P < 0.05) smaller MAX values. None of the treatments showed MAX values exceeding one-third of the 17β-estradiol applied.     

Biochemical modifications in Pinus pinaster Ait. as a result of environmental pollution

Exposure to chemical pollution can cause significant damage to plants by imposing conditions of oxidative stress. Plants combat oxidative stress by inducing antioxidant metabolites, enzymatic scavengers of activated oxygen and heat shock proteins. The accumulation of these proteins, in particular heat shock protein 70 and heme oxygenase, is correlated with the acquisition of thermal and chemical adaptations and protection against oxidative stress. In this study, we used Pinus pinaster Ait. collected in the areas of Priolo and Aci Castello representing sites with elevated pollution and reference conditions, respectively. The presence of heavy metals and the levels of markers of oxidative stress (lipid hydroperoxide levels, thiol groups, superoxide dismutase activity and expression of heat shock protein 70, heme oxygenase and superoxide dismutase) were evaluated, and we measured in field-collected needles the response to environmental pollution. P. pinaster Ait. collected from a site characterized by industrial pollution including heavy metals had elevated stress response as indicated by significantly elevated lipid hydroperoxide levels and decreased thiol groups. In particular, we observed that following a chronic chemical exposure, P. pinaster Ait. showed significantly increased expression of heat shock protein 70, heme oxygenase and superoxide dismutase. This increased expression may have protective effects against oxidative stress and represents an adaptative cellular defence mechanism. These results suggest that evaluation of heme oxygenase, heat shock protein 70 and superoxide dismutase expression in P. pinaster Ait. could represent a useful tool for monitoring environmental contamination of a region and to better understand mechanisms involved in plant defence and stress tolerance.     

Expert knowledge-based assessment of farming practices for different biotic indicators using fuzzy logic

The study presented here describes a modeling approach for the ex-ante assessment of farming practices with respect to their risk for several single-species biodiversity indicators. The approach is based on fuzzy-logic techniques and, thus, is tolerant to the inclusion of sources of uncertain knowledge, such as expert judgment into the assessment. The result of the assessment is a so-called Index of Suitability (IS) for the five selected biotic indicators calculated per farming practice. Results of IS values are presented for the comparison of crops and for the comparison of several production alternatives per crop (e.g., organic vs. integrated farming, mineral vs. organic fertilization, and reduced vs. plow tillage). Altogether, the modeled results show that the different farming practices can greatly differ in terms of their suitability for the different biotic indicators and that the farmer has a certain scope of flexibility in opting for a farming practice that is more in favor of biodiversity conservation. Thus, the approach is apt to identify farming practices that contribute to biodiversity conservation and, moreover, enables the identification of farming practices that are suitable with respect to more than one biotic indicator.