Acoustical signals in the dance language of the giant honeybee,Apis dorsata

Summary Acoustical signals emitted by dancing bees have recently been shown to transmit information about the location of food sources in the western honeybee, Apis mellifera. Towne (1985) reported that in the Asian honeybee species Apis dorsata, which builds a single comb in the open under overhanging rocks or tree branches, sound signals were not emitted by the dancers. This led to the conclusion that acoustical communication is restricted to bees that nest in the dark, like A. mellifera. Here we show that in fact A. dorsata produces dance sounds similar to those emitted by A. mellifera, and that these acoustical signals contain information about distance, direction and profitability of food sources. The acoustical transfer of information has thus evolved independently of nesting in dark cavities. The significance of nocturnal activity in Apis dorsata for the evolution of sound communication is discussed. Correspondence to: W.H. Kirchner     

Biotic and abiotic transformations of methyl tertiary butyl ether (MTBE)

Background Methyl tertiary butyl ether (MTBE) is a fuel additive which is used all over the world. In recent years it has often been found in groundwater, mainly in the USA, but also in Europe. Although MTBE seems to be a minor toxic, it affects the taste and odour of water at concentrations of < 30 μg/L. Although MTBE is often a recalcitrant compound, it is known that many ethers can be degraded by abiotic means. The aim of this study was to examine biotic and abiotic transformations of MTBE with respect to the particular conditions of a contaminated site (former refinery) in Leuna, Germany. Methods Groundwater samples from wells of a contaminated site were used for aerobic and anaerobic degradation experiments. The abiotic degradation experiment (hydrolysis) was conducted employing an ion-exchange resin and MTBE solutions in distilled water. MTBE, tertiary butyl formate (TBF) and tertiary butyl alcohol (TBA) were measured by a gas chromatograph with flame ionisation detector (FID). Aldehydes and organic acids were respectively analysed by a gas chromatograph with electron capture detector (ECD) and high-performance ion chromatography (HPIC). Results and Discussion Under aerobic conditions, MTBE was degraded in laboratory experiments. Only 4 of a total of 30 anaerobic experiments exhibited degradation, and the process was very slow. In no cases were metabolites detected, but a few degradation products (TBF, TBA and formic acid) were found on the site, possibly due to the lower temperatures in groundwater. The abiotic degradation of MTBE with an ion-exchange resin as a catalyst at pH 3.5 was much faster than hydrolysis in diluted hydrochloric acid (pH 1.0). Conclusion Although the aerobic degradation of MTBE in the environment seems to be possible, the specific conditions responsible are widely unknown. Successful aerobic degradation only seems to take place if there is a lack of other utilisable compounds. However, MTBE is often accompanied by other fuel compounds on contaminated sites and anaerobic conditions prevail. MTBE is often recalcitrant under anaerobic conditions, at least in the presence of other carbon sources. The abiotic hydrolysis of MTBE seems to be of secondary importance (on site), but it might be possible to enhance it with catalysts. Recommendation and Outlook MTBE only seems to be recalcitrant under particular conditions. In some cases, the degradation of MTBE on contaminated sites could be supported by oxygen. Enhanced hydrolysis could also be an alternative. - * The basis of this peer-reviewed paper is a presentation at the 9th FECS Conference on 'Chemistry and Environment', 29 August to 1 September 2004, Bordeaux, France.     

Bioaccumulation of selected organochlorines in bats and tits: Influence of chemistry and biology

We analyzed six organochlorine compounds (HCB, lindane, p,p’-DDE, PCB # 138, #153, and # 180) from populations of the European great tit (Parus major) and from five species of bats (Pipistrellus pipistrellus, Nyctalus noctula, Plecotus auritus, Plecotus austriacus, Myotis myotis). A weak positive correlation between K_ow (n-octanol /water partition coefficient) and bioaccumulation (measured as residue values) was found within each species. However, the ratio of the concentrations in bats compared to titmice increased significantly with increasing K_ow. We argue that this is explained by the intensive juvenile feeding through long-term lactation and thus by the more intensive transfer of strongly lipophilic xenobiotics from mother to juvenile in mammals compared to birds. Further, there were clear variations between bat species with ranges of up to 11 times the lowest value for a single compound. These differences are related to different feeding habits of the adults and to different species-specific life history traits, such as age at maturity and number of offspring.     

Genotype-specific responses of fluctuating asymmetry and of preadult survival to the effects of lead and temperature stress in Drosophila melanogaster

Although fluctuating asymmetry (FA) increases with exposure to certain types of environmental stressors such as temperature extremes, relatively little is known about the effects of interaction (e.g., synergism) between known sources of environmental stress on FA. Knowledge of such interaction effects, and of the magnitude of genotype-by-environment interaction, are of fundamental importance toward predicting the usefulness of FA as a bioindicator of environmental pollution. We tested for synergistic effects on FA between elevated temperature and exposure to lead, and examined FA responses simultaneously in four genetic strains of Drosophila melanogaster known to differ in their degree of developmental instability, and presumably in their buffering capacity. In the absence of heavy metal, bristle FA increased with temperature, but in the presence of lead, FA at high temperature (30 (degrees)C) was reduced to levels similar, or below, that at lower temperature (25 (degrees)C). This temperature by lead interaction was statistically significant, but paradoxical in that the disruptive effects of temperature appeared to be attenuated in the presence of the heavy metal. In no case was there a significant effect of lead on bristle FAs, despite documented assimilation of heavy metal by flies, and in no case was the genotype by environment interaction significant. Whereas lead treatment did not influence survival, survival was reduced at the high temperature, but significantly so only in one genetic strain (Oregon-R). There was no relationship between survival and FA across stress treatments within lines. Thus, any disproportionate stress-induced mortality in developmentally unstable classes (developmental selection) was unlikely to bias the FA results. Our results underscore the need for independent replication of significant findings before FA-based biomonitoring can be responsibly and effectively implemented. The results call for caution in using FA as a biomarker of stress, because stress factors may interact in complex and unpredictable ways, which could result in erroneous conclusions about real levels of stress present in field populations, under the unduly simplistic assumption that stress factors will act additively to increase FA.     

Resistance of nutrient cycling systems to perturbations of the flow rates

The resistance of a nutrient cycling system to a disturbance that alters the flow rates, measured by the displacement of the nutrient levels while the disturbance continues, is as important an aspect of stability as the rate of return to equilibrium after the disturbance ends. Two important characteristics of the resistance of a system are the ultimate displacement that would occur if the disturbance lasted indefinitely, and the rate at which the system approaches the ultimate displacement.The mathematical theory is worked out for linear systems and applied to a group of eight nutrient cycling models. The relative resistance of individual compartments of different systems depends heavily on the period of interest, because of the wide range of magnitudes of the eigenvalues, which determine the response times. However, when measuring the change in total nutrients in a system with small exterior flows compared with the amount of internal cycling, only the critical root is significant in determining the response rate.     

Terrestrische ökotoxikologische Testmethoden für die Tropen

Goal, Scope, and Background

Soil organisms play a crucial role in terrestrial ecosystems. Plant Protection Products (PPPs) are known to affect these organisms and might have negative impacts on soil functions influenced by these organisms. Little research has been done to day on the impact of PPPs on tropical ecosystems. Therefore, in this study it was investigated whether the effects of pesticides differ between tropical and temperate regions and whether data generated under temperate conditions can be used for the Environmental Risk Assessment (ERA) in tropical regions.

Methods

The effects of one fungicide (Carbendazim) and one insecticide (lambda-Cyhalothrin) on soil invertebrates (earthworms and arthropods) and functional parameters (organic matter (OM) breakdown, feeding rate) were evaluated in semi-field tests using Terrestrial Model Ecosystems (TMEs) and in field tests using litter-bags. The soil for the TMEs was extracted from the same site near Manaus (Amazonas, Brazil) where the field test was conducted (see Part 1, Römbke et al. 2005 〈DOI: http://dx.doi.org/10.1065/uswf2004.12.088.1〉). The soil was acid clay, typical for tropical regions. In the TMEs the standard test species as well as native tropical species were introduced. Several application scenarios, selected according to their relevance for practical conditions, were realized in TME-tests and field (duration: 150 and 365 days, respectively). Finally, an Environmental Risk Assessment (ERA) for the two chemicals was performed.

Results and Conclusions

The results from the TME-tests show that both test chemicals (depending on their concentration) had toxic effects on the tested organisms and organic matter breakdown, but not on the feeding rates. In the field organic matter breakdown was affected for some time too, but effects on single species were, with one exception (the native earthworm Andiorrhinus amazonicus) not observed; a result probably influenced by the small size of the test plots. Observed differences in OM breakdown between control and treated TMEs were often significant, while due to the higher variability even with differences >10% between means this was not the case in the field.

Recommendation and Perspective

According to the preliminary results of the Environmental Risk, Assessment presented here, the use of the three model chemicals in the tropics (e.g. in Brazil) can cause a damage to the environmental compartment soil. Therefore, a more detailed investigation of these PPPs is recommended, in particular a better evaluation of the exposure (residue analysis). In general, the work performed in Amazonia can be summarised as follows: (1) Depending on the properties of the PPP, the effects of pesticides in tropical resp, temperate regions can differ. (2) If a PPP is going to be used in the tropics, data from tests modified for these conditions should be performed and their results should be incorporated in an Environmental Risk Assessment (ERA) for tropical regions.     

Anthropogenic threats to evolutionary heritage of angiosperms in the Netherlands through an increase in high-competition environments

Present biodiversity comprises the evolutionary heritage of Earth's epochs. Lineages from particular epochs are often found in particular habitats, but whether current habitat decline threatens the heritage from particular epochs is unknown. We hypothesized that within a given region, humans threaten specifically habitats that harbor lineages from a particular geological epoch. We expect so because humans threaten environments that dominated and lineages that diversified during these epochs. We devised a new approach to quantify, per habitat type, diversification of lineages from different epochs. For Netherlands, one of the floristically and ecologically best-studied regions, we quantified the decline of habitat types and species in the past century. We defined habitat types based on vegetation classification and used existing ranking of decline of vegetation classes and species. Currently, most declining habitat types and the group of red-listed species are characterized by increased diversification of lineages dating back to Paleogene, specifically to Paleocene-Eocene and Oligocene. Among vulnerable habitat types with large representation of lineages from these epochs were sublittoral and eulittoral zones of temperate seas and 2 types of nutrient-poor, open habitats. These losses of evolutionary heritage would go unnoticed with classical measures of evolutionary diversity. Loss of heritage from Paleocene-Eocene became unrelated to decline once low competition, shade tolerance, and low proportion of non-Apiaceae were accounted for, suggesting that these variables explain the loss of heritage from Paleocene-Eocene. Losses of heritage from Oligocene were partly explained by decline of habitat types occupied by weak competitors and shade-tolerant species. Our results suggest a so-far unappreciated human threat to evolutionary heritage: habitat decline threatens descendants from particular epochs. If the trends persist into the future uncontrolled, there may be no habitats within the region for many descendants of evolutionary ancient epochs, such as Paleogene.     

Social parasitism by honeybee workers (Apis mellifera capensis Esch.): evidence for pheromonal resistance to host queen’s signals

Social parasites exploit their host’s communication system to usurp resources and reproduce. In the honeybee, Apis mellifera, worker reproduction is regulated by pheromones produced by the queen and the brood. Workers usually reproduce when the queen is removed and young brood is absent. However, Cape honeybee workers, Apis mellifera capensis, are facultative intraspecific social parasites and can take over reproduction from the host queen. Investigating the manner in which parasitic workers compete with host queens pheromonally can help us to understand how such parasitism can evolve and how reproductive division of labour is regulated. In A. m. capensis, worker reproduction is associated with the production of queen-like pheromones. Using pheromonal contest experiments, we show that Apis mellifera scutellata queens do not prevent the production of queen-like mandibular gland compounds by the parasites. Given the importance of these pheromones in acquiring reproductive status, our data suggest that the single invasive lineage of parasitic workers occurring in the range of A. m. scutellata was selected for its superior ability to produce these signals despite the presence of a queen. Such resistance was indeed less frequent amongst other potentially parasitic lineages. Resistance to reproductive regulation by host queens is probably the key factor that facilitates the evolution of social parasitism by A. m. capensis workers. It constitutes a mechanism that allows workers to evade reproductive division of labour and to follow an alternative reproductive option by acquiring direct fitness in foreign colonies instead of inclusive fitness in their natal nests.     

Electrosensory basis for individual recognition in a weakly electric, mormyrid fish, Pollimyrus adspersus (Günther, 1866)

Pollimyrus adspersus discriminates the individually variable waveforms of Electric Organ Discharges (EODs) of conspecifics of only 150–250 s duration. We examined: (1) the discrimination threshold for artificially generated EODs of similar waveform, (2) the mechanism of signal analysis (spectral vs temporal) present, by determining the discrimination between different waveforms of identical amplitude spectra, and (3) the threshold field intensity and reach of discrimination. The triphasic P. adspersus EOD waveform was artificially generated by superimposing two Gaussians, one wide, the second narrow, inverted, and of threefold amplitude. The natural variability among individual EOD waveforms was simulated by phase-shifting one Gaussian relative to the other. The symmetrical waveform where the peaks of the two Gaussians coincided was used as a reference (phase shift=0, rewarded stimulus S+). Results were: (1) in food-rewarded conditioning experiments, trained fish (N=7) detected a phase-shift in artificial EOD stimuli as low as 2 s (N=2 fish), 6 s (N=1) and 10 s (N=1). (2) All fish tested (N=3) discriminated between artificial EODs of identical amplitude spectra but different waveforms (hence, different phase spectra), demonstrating a temporal mechanism of signal analysis. (3) The maximum reach of waveform discrimination was 130 cm at 4.9 V_p-p/cm and 100 S/cm water conductivity (test signal generated at natural amplitude), that is, similar to the reach of EOD detection. Therefore, among the three kinds of electroreceptor organ present in mormyrids, we consider Knollenorgane the relevant sensory organs for EOD waveform discrimination.Communicated by J. Krause     

THE PRACTICE OF COMBINED SEWER SYSTEM MODELING1

ABSTRACT: A combined sewer system is a complex system subject to the dynamic stimuli of precipitation, runoff pollution loads, and sanitary wastes. The system response is a random series of pollutant loads to a receiving water. In many localities, these discharges create considerable water quality problems. Mathematical models can and have played a useful role in predicting the behavior of combined sewer systems and evaluating abatement strategies. Based on the authors' experiences over the past several years, this paper explores some of the positive and negative aspects of using deterministic mathematical models to simulate the behavior of combined sewer systems. A case study follows a discussion on modeling objectives, limitations of modeling, model selection, establishing model credibility, and many other considerations necessary in modeling and simulating these systems.