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Up to now, sediment studies have mainly used standardized aquatic bioassays with organisms of the three trophic levels. Among these, algae are the only representatives of the entire plant kingdom. Bioassays with higher plants have hardly been used for the assessment of sediments. The aim of the present study is to demonstrate that the use of higher plants in biotests for analyses of anthropogenically contaminated sediments yields valuable results, which may be included in a concept for the integrated assessment of waters. The contamination by environmental chemicals was determined in the River Elbe by means of samples taken at the Fahlberg-List industrial site at Magdeburg in combination with studies in a reference area on the Müritz-Elde Waterway (Eldenburger Kanal). Sediment extracts and pore waters were tested with the aquatic lemna test. For comparison, first sediment contact tests withLemna minor were tried out. The whole extract of the sediment from Fahlberg-List Magdeburg and one fraction F5 caused distinct damages of the plants. Obviously, the phytotoxic substances of the whole extract are preferentially concentrated in the highly polar fraction F5. A comparison with chemical analytical data proved that the whole fraction F0 and the fraction F5 were heavily contaminated with chemicals. Assays with pore water from this sediment and with the whole sediment also caused a strong inhibition of growth. These results confirm the high contamination of the sediment from Magdeburg. The tests with the reference sediment suggested only a moderate toxic contamination. The results of this study prove that the selected aquatic plant,Lemna minor, is basically able to indicate contamination. In the aquatic test of the sediment extracts, it showed weak, but very selective, responses to certain classes of contaminants. Fractionating of the sample and subsequent chemical analysis combined with toxicity tests allow to narrow down the groups of substances causing toxic effects. This toxicity was confirmed by analyses of the pore waters and whole sediment samples. Together with other toxicity tests (e.g. standardized bioassays) and combined with biological benthos examinations, an overall judgment can be given for the integrated assessment of waters. 相似文献
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Frank Strieder Claus Rolfs Claudio Spitaleri Pietro Corvisiero 《Die Naturwissenschaften》2001,88(11):461-467
It is in the nature of astrophysics that many of the processes and most of the objects one tries to understand are physically inaccessible. Thus, it is important that those aspects that can be studied in the laboratory be rather well understood. The electron-screening effect on fusion reactions addresses one such aspect: the observed effect under laboratory conditions is presently not understood. 相似文献
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Background and aim Aquatic organisms of the three trophic levels (producers, consumers, reducers) have been used for a long time for ecotoxicological assessments of water quality (HABAB 2000; HABAK 1999). In biotest systems that have become established standards, algae, daphnia, and luminescent bacteria as test organisms are often used so far. However, comparative studies have shown that algae are not sufficiently sensitive to all phytotoxic agents and thus cannot be the exclusive indicator organism of any toxic effects on autotrophic life. Moreover, the EU Water Framework Directive (EU-WFD 2000) sets macrobenthos, fish, phytoplankton, and macrophytes equally side by side as indicators of the status of waters. Nevertheless, biotest systems relying on higher plants have been rarely used in assessments of aquatic sediments so far. Against this background, the aquatic duckweed test (DIN EN ISO 20079) was developed and standardized, and a sediment contact test was developed with Myriophyllum aquaticum (Feiler et al. 2004). The latter was subject of a joint research project of the German Federal Ministry of Education and Research (BMBF-Verbundprojekt “SeKT”) that examined and compared several sediment contact tests (Feiler et al. 2005). Compared to tests with algae, a biotest using macrophytes has the advantage to be more representative of higher plants, since target organism and test organism are closer related in terms of evolutionary history. The present study demonstrates that the application of biotests with higher plants in analyses of contaminated sediments yields valuable results that may contribute to a concept for the integrative assessment of water quality. 相似文献