Mycorrhizal fungal identity and diversity relaxes plant-plant competition

There is a great interest in ecology in understanding the role of soil microbial diversity for plant productivity and coexistence. Recent research has shown increases in species richness of mutualistic soil fungi, the arbuscular mycorrhizal fungi (AMF), to be related to increases in aboveground productivity of plant communities. However, the impact of AMF richness on plant-plant interactions has not been determined. Moreover, it is unknown whether species-rich AMF communities can act as insurance to maintain productivity in a fluctuating environment (e.g., upon changing soil conditions). We tested the impact of four different AMF taxa and of AMF diversity (no AMF, single AMF taxa, and all four together) on competitive interactions between the legume Trifolium pratense and the grass Lolium multiflorum grown under two different soil conditions of low and high sand content. We hypothesized that more diverse mutualistic interactions (e.g., when four AMF taxa are present) can ease competitive effects between plants, increase plant growth, and maintain plant productivity across different soil environments. We used quantitative PCR to verify that AMF taxa inoculated at the beginning of the experiment were still present at the end. The presence of AMF reduced the competitive inequality between the two plant species by reducing the growth suppression of the legume by the grass. High AMF richness enhanced the combined biomass production of the two plant species and the yield of the legume, particularly in the more productive soil with low sand content. In the less productive (high sand content) soil, the single most effective AMF had an equally beneficial effect on plant productivity as the mixture of four AMF. Since contributions of single AMF to plant productivity varied between both soils, higher AMF richness would be required to maintain plant productivity in heterogeneous environments. Overall this work shows that AMF diversity promotes plant productivity and that AMF diversity can act as insurance to sustain plant productivity under changing environmental conditions.     

Biodegradation of superabsorbent polymers in soil

Biodegradation of two superabsorbent polymers, a crosslinked, insoluble polyacrylate and an insoluble polyacrylate/ polyacrylamide copolymer, in soil by the white-rot fungus, Phanerochaete chrysosporium was investigated. The polymers were both solubilized and mineralized by the fungus but solubilization and mineralization of the copolymer was much more rapid than of the polyacrylate. Soil microbes poorly solublized the polymers and were unable to mineralize either intact polymer. However, soil microbes cooperated with the fungus during polymer degradation in soil, with the fungus solubilizing the polymers and the soil microbes stimulating mineralization. Further, soil microbes were able to significantly mineralize both polymers after solubilization by P. chrysosporium grown under conditions that produced fungal peroxidases or cellobiose dehydrogenase, or after solubilization by photochemically generated Fenton reagent. The results suggest that biodegradation of these polymers in soil is best under conditions that maximize solubilization.     

Genetic relationships within and between clonal and solitary forms of the sea anemone Anthopleura elegantissima revisited: evidence for the existence of two species

Along the temperate Pacific coast of North America, the actiniarian sea anemone Anthopleura elegantissima exhibits two discrete life-history phenotypes. Although both forms sexually produce planula larvae, the clonal morph can also asexually propagate by fission, whereas the solitary morph does not. Whether the two forms constitute one or two species has long been contested. Hand originally designated the two forms as conspecifics, whereas Francis – on the basis of differences in microhabitat, biogeographic range and phenotypic frequencies – argued that the two forms constituted a sibling-species pair. From the results of an electrophoretic survey in which they pooled allelic frequencies across several geographic locations, Smith and Potts subsequently argued that the two forms were not genetically differentiated, and therefore represented a single species. We re-examined the relationship between the forms electrophoretically, substantially extending the geographic range and doubling the sample sizes beyond those used by Smith and Potts, and not pooling allelic frequencies in our analyses. Our analysis of patterns of genetic variation at ten highly polymorphic allozyme loci shows that although no fixed genetic differences distinguish the two forms, there are significant differences in allele frequencies between clonal and solitary A.␣elegantissima at every site we sampled throughout their range of sympatry (over 1000 km); within each form, however, there is little detectable genetic differentiation among populations. We therefore conclude that the two forms represent recently reproductively isolated taxa, and propose that the clonal form retain the binomial A. elegantissima (Brandt, 1835), whereas the solitary form be described and named a new species, Anthopleura sp. Received: 28 August 1996 / Accepted: 25 September 1996     

A multispecies test of source–sink indicators to prioritize habitat for declining populations

For species at risk of decline or extinction in source–sink systems, sources are an obvious target for habitat protection actions. However, the way in which source habitats are identified and prioritized can reduce the effectiveness of conservation actions. Although sources and sinks are conceptually defined using both demographic and movement criteria, simplifications are often required in systems with limited data. To assess the conservation outcomes of alternative source metrics and resulting prioritizations, we simulated population dynamics and extinction risk for 3 endangered species. Using empirically based habitat population models, we linked habitat maps with measured site‐ or habitat‐specific demographic conditions, movement abilities, and behaviors. We calculated source–sink metrics over a range of periods of data collection and prioritized consistently high‐output sources for conservation. We then tested whether prioritized patches identified the habitats that most affected persistence by removing them and measuring the population response. Conservation decisions based on different source–sink metrics and durations of data collection affected species persistence. Shorter time series obscured the ability of metrics to identify influential habitats, particularly in temporally variable and slowly declining populations. Data‐rich source–sink metrics that included both demography and movement information did not always identify the habitats with the greatest influence on extinction risk. In some declining populations, patch abundance better predicted influential habitats for short‐term regional persistence. Because source–sink metrics (i.e., births minus deaths; births and immigrations minus deaths and emigration) describe net population conditions and cancel out gross population counts, they may not adequately identify influential habitats in declining populations. For many nonequilibrium populations, new metrics that maintain the counts of individual births, deaths, and movement may provide additional insight into habitats that most influence persistence.     

Prenatal diagnosis of 47,XX,der(15)t(15;16)(q13;p13.2)

A case of prenatally detected partial trisomy 15 and 16 is reported. Amniocentesis was performed at 14 weeks' gestation because a 6-mm nuchal translucency was detected on a dating ultrasound evaluation. Karyotype from amniocytes was suspect of an aberration concerning a marker chromosome. FISH analysis demonstrated that this marker chromosome was a der(15). A maternal chromosomal rearrangement t(15;16)(q13;p13.2) was confirmed. At birth, the proband was severely hydropic and had dysmorphic features, which included hypertelorism, micrognathia, incomplete separation of the maxilla and mandible, hyperflexed hands with overlapping fingers, hyposegmented right lung, and a single umbilical artery. Copyright © 2004 John Wiley & Sons, Ltd.     

Fluometuron and pendimethalin runoff from strip and conventionally tilled cotton in the southern atlantic coastal plain

In the Atlantic Coastal Plain region of southern Georgia (USA), cotton (Gossypium hirsutum L.) acreage increased threefold in the past decade. To more effectively protect water quality in the region, best management practices are needed that reduce pesticide runoff from fields in cotton production. This study compared runoff of two herbicides, fluometuron [N,N-dimethyl-N'-[3-(trifluoromethyl)-phenyl]-urea] and pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitro-benzenamine], from plots in strip-tillage (ST) and conventional-tillage (CT) management near Tifton, GA. Rainfall simulations were conducted one day after preemergence herbicide applications to 0.0006-ha plots and runoff from 0.15-ha plots due to natural rainfall following preemergence pendimethalin and fluometuron and postemergence fluometuron use was monitored. Pendimethalin runoff was greater under CT than ST due to strong pendimethalin soil sorption and higher erosion and runoff under CT. The highest losses, 1.3% of applied in CT and 0.22% of applied in ST, were observed during rainfall simulations conducted 1 DAT. Fluometuron runoff from natural rainfall was substantially lower from ST than from CT plots but the trend was reversed in rainfall simulations. In all studies, fluometuron runoff was also relatively low (<1% of applied), and on plots under natural rainfall, desmethylfluometuron (DMF) represented about 50% of total fluometuron runoff. Fluometuron's relatively low runoff rate appeared linked to its rapid leaching, and high DMF detection rates in runoff support DMF inclusion in fluometuron risk assessments. Results showed that ST has the potential to reduce runoff of both herbicides, but fluometuron leaching may be a ground water quality concern.     

Ammonia,methane, and nitrous oxide emission from pig slurry applied to a pasture in New Zealand

Much animal manure is being applied to small land areas close to animal confinements, resulting in environmental degradation. This paper reports a study on the emissions of ammonia (NH3), methane (CH4), and nitrous oxide (N2O) from a pasture during a 90-d period after pig slurry application (60 m3 ha-1) to the soil surface. The pig slurry contained 6.1 kg total N m-3, 4.2 kg of total ammoniacal nitrogen (TAN = NH3 + NH4) m-3, and 22.1 kg C m-3, and had a pH of 8.14. Ammonia was lost at a fast rate immediately after slurry application (4.7 kg N ha-1 h-1), when the pH and TAN concentration of the surface soil were high, but the loss rate declined quickly thereafter. Total NH3 losses from the treated pasture were 57 kg N ha-1 (22.5% of the TAN applied). Methane emission was highest (39.6 g C ha-1 h-1) immediately after application, as dissolved CH4 was released from the slurry. Emissions then continued at a low rate for approximately 7 d, presumably due to metabolism of volatile fatty acids in the anaerobic slurry-treated soil. The net CH4 emission was 1052 g C ha-1 (0.08% of the carbon applied). Nitrous oxide emission was low for the first 14 d after slurry application, then showed emission peaks of 7.5 g N ha-1 h-1 on Day 25 and 15.8 g N ha-1 h-1 on Day 67, and decline depending on rainfall and nitrate (NO3) concentrations. Emission finally reached background levels after approximately 90 d. Nitrous oxide emission was 7.6 kg N ha-1 (2.1% of the N applied). It is apparent that of the two major greenhouse gases measured in this study, N2O is by far the more important tropospheric pollutant.