Pyrosequencing reveals bacteria carried in different wind-eroded sediments

Little is known about the microbial communities carried in wind-eroded sediments from various soil types and land management systems. The novel technique of pyrosequencing promises to expand our understanding of the microbial diversity of soils and eroded sediments because it can sequence 10 to 100 times more DNA fragments than previous techniques, providing enhanced exploration into what microbes are being lost from soil due to wind erosion. Our study evaluated the bacterial diversity of two types of wind-eroded sediments collected from three different organic-rich soils in Michigan using a portable field wind tunnel. The wind-eroded sediments evaluated were a coarse sized fraction with 66% of particles >106 μm (coarse eroded sediment) and a finer eroded sediment with 72% of particles <106 μm. Our findings suggested that (i) bacteria carried in the coarser sediment and fine dust were effective fingerprints of the source soil, although their distribution may vary depending on the soil characteristics because certain bacteria may be more protected in soil surfaces than others; (ii) coarser wind-eroded sediment showed higher bacterial diversity than fine dust in two of the three soils evaluated; and (iii) certain bacteria were more predominant in fine dust (, , and ) than coarse sediment ( and ), revealing different locations and niches of bacteria in soil, which, depending on wind erosion processes, can have important implications on the soil sustainability and functioning. Infrared spectroscopy showed that wind erosion preferentially removes particular kinds of C from the soil that are lost via fine dust. Our study shows that eroded sediments remove the active labile organic soil particulates containing key microorganisms involved in soil biogeochemical processes, which can have a negative impact on the quality and functioning of the source soil.     

Shadow attenuation with high dynamic range images

Shadow often interferes with accurate image analysis. To mitigate shadow effects in near-earth imagery (2 m above ground level), we created high dynamic range (HDR) nadir images and used them to measure grassland ground cover. HDR composites were created by merging three differentially exposed images spanning a wide exposure range and resulted in lightened shadows. HDR images showed more detail; reduced the numbers of pure black, pure white, and pixels visually indistinguishable from black and white; reapportioned skewed luma values towards a normal distribution; and increased the Euclidean distance between litter and bare ground RGB values--allowing increased feature separation; all of which facilitated an increase in real feature classification through manual image analysis. Drawbacks to the method included decreased image sharpness due to minor misalignment of images or moving vegetation, time required to create HDR images, and difficulty with acquiring primary images from a moving platform. We conclude that HDR imagery can provide more accurate measurements of bare soil cover for ecosystem monitoring and assessment.     

Acceptable Cancer Risks: Probabilities and Beyond

The acceptability of cancer risk requires consideration of factors that extend beyond mere numerical representations, such as either individual lifetime risk in excess of background and excess incidence. Recently, use of these numbers has been tempered by the addition of qualitative weights-of-evidence that describe the degree of support provided by animal and epidemiologic results. Nevertheless, many other factors, most of which are not quantitative, require incorporation but remain neglected by the analyst eager to use quantitative results.

In this paper we show that simple risk measures are often fraught with problems. Moreover, these measures do not incorporate the very essence of acceptability, which includes notions of responsibility, accountability, equity, and procedural legitimacy, among others. We link the process of risk assessment to those legal and regulatory standards that shape it. These standards are among the principal means to resolve risk-related disputes and to enhance the balancing of competing interests when science and law meet on uncertain and often conjectural ground.

We conclude the paper with a proposal for the portfolio approach to manage cancer risks and to deal with uncertain scientific information. This approach leads to the concept of "provisional acceptability," which reflects the choices available to the decisionmaker, and the trade-offs inherent to such choices.     

Waste Streams,Technology, and State Requirements

Biomedical wastes are generated by hospitals, laboratories, animal research facilities, and by other institutional sources. The disposal of these wastes is coming under critical public scrutiny, and regulations are being promulgated to control their disposal. Incineration is not a final disposal method since it generates a solid residue (ash) which must be buried or otherwise disposed. The incineration process, however, renders the waste non-toxic, non-hazardous, and non-putrescible, and reduces the volume of material for ultimate disposal by an order of magnitude. In some instances, the residue may have high levels of heavy metals; however, this is the exception rather than the rule.     

Sample Design Considerations for Indoor Air Exposure Surveys

Concern about the potential for indoor air pollution has prompted recent surveys of radon and NO₂ concentrations In homes and personal exposure studies of volatile organics, carbon monoxide and pesticides, to name a few. The statistical problems in designing sample surveys that measure the physical environment are diverse and more complicated than those encountered in traditional surveys of human attitudes and attributes. This paper addresses issues encountered when designing indoor air quality (IAQ) studies. General statistical concepts related to target population definition, frame creation, and sample selection for area household surveys and telephone surveys are presented. The implications of different measurement approaches are discussed, and response rate considerations are described.     

Back to Basics

ABSTRACT

Tests of the compatibility of geomembrane (GM) samples with waste were conducted using U.S. Environmental Protection Agency (EPA) Method 9090 and the Comprehensive Testing System (CTS). The CTS is a multi-axial performance test capable of simultaneous cyclic mechanical loads and chemical exposure. The test chemicals consisted of solvents, transportation-related compounds, and synthesized landfill leachate. Method 9090 testing was unable to distinguish between the effects of individual chemicals to which the GM was subjected, while the CTS was able to provide statistically-significant differences that were also traceable to chemical properties of the solvent and the GM liner. Further, the time required for changes in mechanical properties of the GM was significantly shorter than would be expected based upon diffusion of the solvent into the GM alone. The combination of chemical attack with mechanical load was found to enhance both reduction in mechanical properties and the ability of the solvent to diffuse into the GM. The CTS is a more realistic test than the existing standard test methods because of its ability to provide multi-axial loads and chemical exposure simultaneously.     

Revised Air Quality Surveillance Requirements for State Implementation Plans

The National Ambient Air Quality Monitoring Program is carried out by state and local air pollution control agencies in support of their State Implementation Plans (SIP’s). The current EPA regulations which specify the characteristics of these state monitoring programs are undergoing change as a result of a comprehensive review by an independent work group. These revised regulations, which are described in the paper, are intended to improve the quality, timeliness, and usability of the data generated by the states for all data users. In addition, the revised regulations seek to bring about; (a) national consistency in monitoring site locations through standardized siting procedures; (b) improved network operations by means of a minimum quality assurance program; (c) reduced network inflexibility through an annual network review process; and (d) reduced data reporting through changed data reporting procedures.     

Ambient Ozone Concentration Patterns among Eastern U.S. Urban Areas Using Factor Analysis

Data on dally maximum ozone concentrations measured at ambient air monitoring stations operated by state and local air pollution control agencies in the Eastern United States were analyzed using principal factor analysis. Four orthogonal factors representing O₃ formation potentials were derived using the statistical package SPSS; these factors accounted for over two-thirds of the variations in 1978 summer O₃ levels at 21 urban-oriented stations. The analysis confirmed that O₃ variations are similar among stations within defined geographical areas; this confirmation supports the widely held theory that ambient O₃ formations are reglonwlde. The analysis suggested that trends analysis for determining general progress in improving O₃ air quality should be based on aggregate statistics from clusters of monitors rather than from a single monitoring station within areas associated with the derived factors.     

Managing Baltic Sea fisheries under contrasting production and predation regimes: ecosystem model analyses

Based on an earlier published ecosystem model, we have explored possible effects of different management scenarios for the Baltic Sea. The scenarios include an oligotrophication of the system, a drastic increase in the number of seals, and changes in the fishery management. From these simulations we conclude that fisheries, seals, and eutrophication all have strong and interacting impacts on the ecosystem. These interactions call for integrated management. The modeling highlights the potential for conflicts among management mandates such as flourishing fisheries, rebuilt seal populations, and substantially reduced eutrophication. The results also suggest that fisheries management reference points have to be adjusted in response to changes in the presence of natural predators or ecosystem productivity.