Ecological implications of anti-pathogen effects of tropical fungal endophytes and mycorrhizae

We discuss studies of foliar endophytic fungi (FEF) and arbuscular mycorrhizal fungi (AMF) associated with Theobroma cacao in Panama. Direct, experimentally controlled comparisons of endophyte free (E-) and endophyte containing (E+) plant tissues in T. cacao show that foliar endophytes (FEF) that commonly occur in healthy host leaves enhance host defenses against foliar damage due to the pathogen (Phytophthora palmivora). Similarly, root inoculations with commonly occurring AMF also reduce foliar damage due to the same pathogen. These results suggest that endophytic fungi can play a potentially important mutualistic role by augmenting host defensive responses against pathogens. There are two broad classes of potential mechanisms by which endophytes could contribute to host protection: (1) inducing or increasing the expression of intrinsic host defense mechanisms and (2) providing additional sources of defense, extrinsic to those of the host (e.g., endophyte-based chemical antibiosis). The degree to which either of these mechanisms predominates holds distinct consequences for the evolutionary ecology of host-endophyte-pathogen relationships. More generally, the growing recognition that plants are composed of a mosaic of plant and fungal tissues holds a series of implications for the study of plant defense, physiology, and genetics.     

Projecting landscape changes in southern Chile: Simulation of human and natural processes driving land transformation

We describe a simulation model representing the most important human and natural factors driving land use and cover changes (LUCC) in southern Chile. We evaluate the model by examining its ability to simulate LUCC observed over the past three decades, conduct a sensitivity analysis of simulated trends to changes in important model parameters, and use the model to project likely landscape transformations over the next decade under “as usual,” “pessimistic,” and four “optimistic” scenarios. The model consists of five submodels representing LUCC on two distinct soil formations (volcanic ash and gleysols) and four major land use categories: native forest, agricultural land, shrubland, and urban land. Land use and cover sub-categories include old growth forests, secondary forests, and low and flooded shrubland. The model simulated well general historic trends in forest cover, agricultural land, shrubland, and urban land: from a forest-dominated landscape in 1976 to a landscape dominated by shrubland and agricultural land by 2007. Forest loss, forest degradation by logging and clearing for agriculture were the most important direct drivers of LUCC: forest logging and clearing were most important from 1976 to 1985, whereas after 1985 logging for firewood, driven by population growth, was most important. Sensitivity analysis indicated that model projections of general trends in the main land use and cover categories were not overly sensitive to changes in important model parameters, although further study is necessary to improve our estimates of the proportion of pasture requirements supplied by clearing low shrubland. Projections of LUCC suggested that a reduced amount of secondary forest would be left by 2017 if no actions are taken to reduce forest loss (“as usual”). Increasing population (“pessimistic scenario”) resulted in similar trajectories than those predicted by the as usual scenario, whereas reducing logging for firewood and increasing forest recruitment from shrubland could reduce loss of native forest by nearly one-third (“optimistic scenarios”). Surprisingly, shrubland exhibited the most complex and influential dynamics in all scenarios, being the immediate outcome of forest loss and the main long-term source of land for agriculture, urban expansion, and forest recovery. Few studies in Chile, or elsewhere, have considered the importance of this intermediate successional stage. Of the scenarios simulated, financial incentives targeted toward channeling shrubland into regenerated forest seemed most promising, although obstacles to such a management strategy exist.     

Polychlorinated biphenyls in two salt marsh sediments of the Venice Lagoon

Polychlorinated biphenyls (PCBs) were measured in two dated salt marsh cores of the Venice Lagoon to assess their input chronology and to evaluate the importance of atmospheric deposition as a source. Sampling sites were chosen in order to evidence the differences between areas located leeward and windward with respect to inputs originating in both the city of Venice and the industrial area. Concentrations of PCB indicators (0.13-15.6 ng g?1) increased gradually from the 1930s, reached maxima from the 1950s to the late 1970s, and then decreased. PCB loadings to marshes are driven by both the atmospheric deposition and the resuspension of subtidal sediments, this latter being more important for heavier congeners. The downwind marsh recorded higher fluxes (0.06-9.72 ng cm?2 year?1) than the upwind one (0.01-0.53 ng cm?2 year?1). Recent fluxes are rather consistent with bulk deposition measurements. A higher contribution of CB-101 and CB-118 was detected in the intermediate layers of the downwind site, suggesting a different PCB source for the corresponding time interval. In the other marsh, PCBs showed a rather constant composition at all levels (mostly CB-153, CB-138 and CB-180), accounting for a regional influence. Deep layers showed an enrichment of higher chlorinated congeners at both sites, whereas recent samples conserve the patterns typical of surficial and subsurficial subtidal sediments. The scientific approach adopted in this research can be considered as a sort of methodological procedure for the determination of fluxes and pathways of PCBs through the study of marsh cores.     

Blood lead levels and nutrition in an urban community in Venezuela

Previous lead (Pb) exposure studies identified the Michelena sector in Valencia as a ‘critical’ area for Pb contamination. An analytical-ecological study was designed to evaluate the association of environmental and nutritional variables with blood lead (BPb) in children attending two schools located in this sector. A questionnaire was administered and the findings were correlated with BPb and serum-calcium (S-Ca) levels. The total population examined included 60 children (4–9 years old). The mean BPb values was 10.5?±?3?ug/dL (61.7%?≥?10?ug/dL) and the mean S-Ca was 8.9?±?0.7?mg/dL. A significant difference (p?=?0.042) was found between BPbs in males (11.1?±?3.1?ug/dL) when compared with females (9.6?±?2.8?ug/dL). Child age, school, schedule at school, parents' occupational exposure, habits, and socioeconomic status had no significant association with BPbs. The relationship between BPb levels (dependent variable) and the S-Ca and daily Ca intake levels (independent variables) was analysed through multiple regression analysis. The results show an inverse relationship between BPb and both variables (β?=??0.073, and β?=??0.001) respectively, with the daily Ca intake being the only significant variable in the model (p?=?0.04). Although average BPbs was not very high, the percentage of children with BPbs higher than the US-CDC intervention level was greater than those found in other studies. Since detailed information is generally unavailable for these children in Venezuela, this study provides insights for future research.     

Invasive salmonids and lake order interact in the decline of puye grande Galaxias platei in western Patagonia lakes

Salmonid fishes, native to the northern hemisphere, have become naturalized in many austral countries and appear linked to the decline of native fishes, particularly galaxiids. However, a lack of baseline information and the potential for confounding anthropogenic stressors have led to uncertainty regarding the association between salmonid invasions and galaxiid declines, especially in lakes, as these have been much less studied than streams. We surveyed 25 lakes in the Aysén region of Chilean Patagonia, including both uninvaded and salmonid-invaded lakes. Abundance indices (AI) of Galaxias platei and salmonids (Salmo trutta and Oncorhynchus mykiss) were calculated using capture-per-unit-effort data from gillnets, minnow traps, and electrofishing. We also measured additional environmental variables, including deforestation, lake morphometrics, altitude, and hydrological position (i.e., lake order). An information-theoretic approach to explaining the AI of G. platei revealed that by far the strongest effect was a negative association with the AI of salmonids. Lake order was also important, and using structural equation modeling, we show that this is an indirect effect naturally constraining the salmonid invasion success in Patagonia. Supporting this conclusion, an analysis of an independent data set from 106 mountain lakes in western Canada showed that introduced salmonids are indeed less successful in low-order lakes. Reproductive failure due to insufficient spawning habitat and harsh environmental conditions could be the cause of these limits to salmonid success. The existence of this effect in Chilean Patagonia suggests that low-order lakes are likely to provide natural ecological refugia for G. platei. Finally, pristine, high-order lakes should be actively protected as these have become rare and irreplaceable unspoiled references of the most diverse, natural lake ecosystems in Patagonia.     

Occurrence of the non-native annual bluegrass on the Antarctic mainland and its negative effects on native plants

Few non-native species have colonized Antarctica, although increased human activity and accelerated climate change may increase their number, distributional range, and effects on native species on the continent. We searched 13 sites on the maritime Antarctic islands and 12 sites on the Antarctic Peninsula for annual bluegrass (Poa annua), a non-native flowering plant. We also evaluated the possible effects of competition between P. annua and 2 vascular plants native to Antarctica, Antarctic pearlwort (Colobanthus quitensis) and Antarctic hairgrass (Deschampsia antarctica). We grew the native species in experimental plots with and without annual bluegrass under conditions that mimicked the Antarctic environment. After 5 months, we measured photosynthetic performance on the basis of chlorophyll fluorescence and determined total biomass of both native species. We found individual specimens of annual bluegrass at 3 different sites on the Antarctic Peninsula during the 2007-2008 and 2009-2010 austral summers. The presence of bluegrass was associated with a statistically significant reduction in biomass of pearlwort and hairgrass, whereas the decrease in biomass of bluegrass was not statistically significant. Similarly, the presence of bluegrass significantly reduced the photosynthetic performance of the 2 native species. Sites where bluegrass occurred were close to major maritime routes of scientific expeditions and of tourist cruises to Antarctica. We believe that if current levels of human activity and regional warming persist, more non-native plant species are likely to colonize the Antarctic and may affect native species.     

Investigation of Motorcyclist Cervical Spine Trauma Using HUMOS Model

Objective: With 16 percent of the total road user fatalities, motorcyclists represent the second highest rate of road fatalities in France after car occupants. Regarding road accidents, a large proportion of trauma was on the lower cervical spine. According to different clinical studies, it is postulated that the cervical spine fragility areas are located on the upper and lower cervical spine. In motorcycle crashes, impact conditions occur on the head segment with various orientations and impact directions, leading to a combination of rotations and compression. Hence, motorcyclist vulnerability was investigated considering many impact conditions. Method: Using the human model for safety (HUMOS), a finite element model, this work aims to provide an evaluation of the cervical spine weaknesses based on an evaluation of injury mechanisms. This evaluation consisted of defining 2 injury risk factors (joint injury and bone fracture) using a design of experiment including various velocities, impact directions, and impact orientations. Results: The results confirmed previously reported clinical and epidemiological work on the fragility of the lower cervical spine and the upper cervical spine segments. Joint injuries appeared before bone fractures on both the upper and lower cervical spine. Bone fracture risk was greater on the lower cervical spine than on the upper cervical spine. The compression induced by a high impact angle was identified as an important injury severity factor. It significantly increased the injury incidence for both joint injuries and bone fractures. It also induced a shift in injury location from the lower to the upper cervical spine. The impact velocity exhibited a linear relationship with injury risks and severity. It also shifted the bone fracture risk from the lower to upper spinal segments.     

Eulerian and lagrangian transport time scales of a tidal active coastal basin

In this work the flushing features of a tidal active coastal basin, the Venice lagoon, have been investigated. The water transport time scale (TTS) has been computed by means of both an eulerian and a lagrangian approach. The obtained results have been compared in order to identify the main differences between the two methods.