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991.
Early development of the Antarctic sea urchin Sterechinus neumayeri was examined under two differ-ent culture regimes: one to simulate development near-bottom (“demersal development”) and the
other to simulate the development of embryos in the water column (“pelagic development”). When embryos of both treatments
reached the hatching blastula stage at 5 d post-fertilization (−1.5 °C), the blastulae that had undergone demersal development
evidenced significant differences (by ANOVA or suitable non-parametric comparison) in the following: a thicker blastoderm
layer (12%, P < 0.001), higher ash-free dry weights (19%, P < 0.01), lower mass-specific respiration rates (50%, P < 0.001), higher incorporation rates of 35S-methionine into protein (23%, P < 0.003), and a differential pattern of protein synthesis. When embryos developed demersally, they remained in the jelly-coat
material released with the eggs at spawning. Quantitative isolation of this jelly-coat material in S. neumayeri demonstrated that it contained a significant amount of organic matter, 115 ng ash-free dry mass per egg, equivalent to 17%
of the egg's initial organic mass. Uptake of external nutrients during embryogenesis may be a significant component of the
physiological energetics of this polar invertebrate by allowing the utilization of jelly-coat material released by a female
during spawning.
Received: 21 April 1999 / Accepted: 5 June 2000 相似文献
992.
F. Maltagliati A. P. Peru M. Casu F. Rossi C. Lardicci M. Curini-Galletti A. Castelli 《Marine Biology》2000,136(5):871-879
The genetic relationships between morphologically indistinguishable marine and brackish populations of Syllis gracilis Grube, 1840 (Polychaeta: Syllidae) were studied by means of allozyme electrophoresis. Samples of S. gracilis from marine coastal and brackish-water habitats were examined for variation at 13 presumptive loci. In addition, a sample
of the closely related species S. prolifera (Krohn, 1852) was analysed. Five loci were multiallelic in at least one population of S. gracilis and eight loci in S. prolifera. Low to moderate levels of within-population genetic variability were found, with average expected heterozygosity values
ranging from H = 0.068 (±0.043 SE) to 0.187 (±0.069 SE) in the populations of S. gracilis; higher values were found in S. prolifera (H = 0.325 ± 0.076). The presence of various private alleles indicated a marked genetic divergence among populations of S. gracilis, with Nei's genetic distances ranging from D = 0.000 to 0.833 and a highly significant F
ST value. Furthermore, evidence for strong genetic heterogeneity between two sympatric marine populations was found. UPGMA cluster
analysis and multidimensional scaling pointed out a clear genetic divergence between brackish and marine populations. At least
two genetically divergent entities occurred in marine and brackish habitats. This could be due to local adaptation of individuals
coming from marine populations to brackish habitats, but more presumably to the occurrence of a species complex within S. gracilis.
Received: 6 June 1999 / Accepted: 7 February 2000 相似文献
993.
We examined the genetic identities of 49 individuals of four species of eels in the genus Anguilla Shaw, A. anguilla, A. japonica, A. reinhardti and A. rostrata, using the random(ly) amplified polymorphic DNA (RAPD)-PCR technique. We used 15 random decamer primers. Out of the 22 148
amplification products detected, 454 markers were evaluated using Nei's distance coefficient, two numerical methods (neighbor-joining,
UPGMA), and phylogenetic analysis using parsimony (PAUP). Both numerical and parsimony methods were congruent and confirmed
the specific status of all four examined Anguilla species. In all dendrograms, all individuals of each of the species clustered together within four closed groups with highly
significant bootstrap values (between 97 and 100%). Additionally 1 to 18 species-specific monomorphic diagnostic fragments
were detected in three of the species; none were detected in A. anguilla. Mean interspecific genetic distances ranged from 0.384 to 0.559, with the minimum between A. anguilla and A. rostrata and the maximum between A. anguilla and A. reinhardti. Individuals collected at one locality and belonging to a single species did not cluster together, and neither were any diagnostic
monomorphic fragments found for individuals of single localities. This probably reflects a random dispersal of larvae in ocean
currents before they reach coastal waters. Among all possible species pairs, only the two Atlantic species were consistently
clustered in all dendrograms, with highly significant bootstrap values (100%). Additionally, we detected ten diagnostic markers
for this pair of eel species. From RAPD data, we suggest a phylogram which was routed by the descending analysis method: A. reinhardti appears to have been the first species to diverge from a putative common ancestor of the four eel species. Later, A. japonica separated from the hypothetical ancestor of the Atlantic species, which then split recently into A. anguilla and A. rostrata. The close relationship of A. anguilla and A. rostrata is indicated by their low genetic distance (GD = 0.384) and a high degree of shared RAPD fragments (SF = 71.2%); this compares with the following means for all other species pairs: ( = 0.531, = 44.2%). The prerequisites for using RAPD data for the reconstruction of phylogenies are discussed.
Received: 2 September 1999 / Accepted: 8 May 2000 相似文献
994.
L. Zane L. Ostellari L. Maccatrozzo L. Bargelloni J. Cuzin-Roudy F. Buchholz T. Patarnello 《Marine Biology》2000,136(2):191-199
Meganyctiphanes norvegica (M. Sars)is a pelagic crustacean that plays a key role in marine food webs of North Atlantic Ocean and marginal seas. We
studied eight population samples collected in the European Atlantic and Mediterranean Sea. By means of single strand conformation
polymorphism analysis (SSCP) and direct sequencing, we investigated a segment of 158 base pairs of the mitochondrial gene
coding for the subunit 1 of NADH dehydrogenase. We found 12 sequence variants among the 385 individuals studied. Analysis
of molecular variance (AMOVA) showed that 14.75% of the total genetic variability was explained by differences between populations,
thus indicating absence of panmixia for these populations. Pairwise comparisons revealed three distinct genetic pools: the
first one represented by Cadiz Bay, the second one by the Ligurian Sea, and the third one included all the NE Atlantic samples.
We also investigated one population from the Alboran Sea (within the Mediterranean basin, east of the Strait of Gibraltar).
This population was found to be genetically intermediate between the NE Atlantic samples and the Ligurian sample, suggesting
that the restriction to the gene flow is not associated with the Strait of Gibraltar, but possibly with the Oran–Almeria oceanographic
front. The present work indicates that M. norvegica, although endowed with a high dispersal capacity because of its pelagic habit, can develop separate breeding units inside
the same oceanic basin (the Atlantic). Furthermore, the Ligurian sample should be considered as a distinct evolutionary entity,
separated from the Atlantic population.
Received: 2 May 1999 / Accepted: 26 November 1999 相似文献
995.
Relatively few insects have invaded the marine environment, and only five species of sea skaters, Halobates Eschscholtz (Hemiptera: Gerridae), have successfully colonized the surface of the open ocean. All five species occur in the
Pacific Ocean, H. germanus White also occurs in the Indian Ocean, whereas H. micans Esch- scholtz is the only species found in the Atlantic Ocean. We sequenced a 780 bp long region of the mitochondrial cytochrome
oxidase subunit I gene (COI) for a total of 66 specimens of the five oceanic Halobates species. Our purpose was to investigate the genetic variation within species and estimate the amount of gene flow between
populations. We defined 27 haplotypes for H. micans and found that haplotype lineages from each of the major oceans occupied by this species are significantly different, having
sequences containing five to seven unique base substitutions. We conclude that gene flow between populations of H. micans inhabiting the Atlantic, Pacific, and Indian Ocean is limited and hypothesize that these populations have been separated
for 1 to 3 million years. Similarly, there may be limited gene flow between H. germanus populations found in the Pacific and Indian Ocean and between H. sericeus populations inhabiting the northern and southern parts of the Pacific Ocean. Finally, we discuss our findings in relation
to recent hypotheses about the influence of oceanic diffusion on the distribution and population structure of oceanic Halobates spp.
Received: 29 July 1999 / Accepted: 23 November 1999 相似文献
996.
The accumulation of lipofuscin, which is an indictor of physiological age, in the brain of juvenile European lobster (Homarus gammarus L.) was monitored for 22 mo in three experimental temperature regimes that simulated seasonal variation in temperature in
the geographic range of this species. Metabolic rate responses to changes in temperature were estimated by measuring the activity
of the electron transport system (ETS) in muscle tissue and in vivo rates of oxygen consumption. Lipofuscin accumulation oscillated
with simulated seasonal changes in temperature and was described by seasonalised von Bertalanffy growth functions. The incremental
accumulation in lipofuscin between sampling dates was linearly related to the number of degree days that accumulated between
dates, irrespective of the amplitude of temperature fluctuation that had occurred. ETS activity increased with acclimation
temperature and was modelled using a polynomial function. This indicated a lower temperature sensitivity in the temperature
mid-range (12 to 16 °C), although the Q10 for this mid-range was 2.1. ETS activity in lobsters acclimated to 8 and 18 °C and assayed at 13 °C was similar, indicating
no compensation for changes in environmental temperature. Oxygen consumption rate was significantly higher at 14 °C than at
10.5 °C and had a Q10 of 3.6, again suggesting no compensation to temperature change. The absence of metabolic compensation in response to temperature
change in H. gammarus is consistent with the predictability of changes in temperature and food availability in the sub-littoral environment of
this species. As lipofuscin accumulates according to metabolic rate, and metabolic rate in H. gammarus is directly correlated with temperature, geographic differences and long-term temporal trends in temperature will need to
be considered when converting physiological age indices, obtained from lipofuscin estimates, to a chronological scale.
Received: 27 April 2000 / Accepted: 21 July 2000 相似文献
997.
The endemic New Zealand echinoid, Evechinus chloroticus (Valenciennes), was sampled approximately monthly from September 1990 to October 1994 at three sites in Tory Channel, Marlborough
Sounds, New Zealand. These channel sites (outer, mid and inner) were up to 20 km from the open ocean and differed in their
shore type, exposure to wave action and macrophyte abundance. E. chloroticus showed an annual reproductive cycle at each site, with gametogenesis commencing in the late austral winter and spawning in
summer. Maximum gonad indices (reproductive potential) varied spatially, with the outer site generally having higher maximum
gonad indices than the inner site, and temporally, with maximum gonad indices occurring earlier in 3 of the 4 seasons at the
outer site than the inner site. The mid and inner sites showed much greater variation in maximum gonad indices (range 15.83
to 26.99% and 11.87 to 20.90%, respectively) than the outer site (range 19.31 to 22.95%). Reproductive output (weight of gametes
released per gram echinoid) also varied, with the different sites showing significantly different outputs in the different
years. A regression of maximum reproductive potential against reproductive output was significant ( p < 0.001), and had a positive slope with an r
2 of 0.79. While, the initiation of gametogenesis was relatively synchronous between sites and years, and is possibly cued
by increasing daylength, it progressed at different rates among populations. Spawning did show spatial and temporal variability,
occurring near the time of highest sea-surface temperatures (∼15 °C). The observed variations in reproductive cycle may be
related to small-scale variability in diet and environmental conditions. Furthermore, asynchronous spawning, variable spawning
duration, and variable reproductive output are likely to strongly influence annual recruitment variability in E. chloroticus, with different larval subpopulations contributing unequally in different years. The ecological consequences of this, both
for the ability of E. chloroticus to propagate itself in space and time and for the management of the developing E. chloroticus fishery in New Zealand, are discussed.
Received: 9 December 1998 / Accepted: 13 June 2000 相似文献
998.
Western sandpiper (Calidris mauri) predation was examined by concurrent experiments and direct observations of foraging behaviour on high intertidal mudflats
of the Fraser River estuary, British Columbia. Western sandpipers foraged by either “pecking” on the surface (64% of observational
time) or probing into sediment (29%). The first experiment (probe-mark method) consisted of collecting small-volume cores
(21.2 cm3) of probed (experimental) and non-probed (control) sediment on the tidal flat, following a 22.5-min feeding period. The second
experiment (exclosure method) involved deploying exclosures immediately prior to the feeding period and subsequent collection
of cores from inside (control) and outside (experimental) the exclosures. Sediment cores were analysed for both macrofaunal
and meiofaunal size fractions. Comparisons between macro- and meiofaunal invertebrate densities in experimental and control
sediments revealed significant differences, attributed to shorebird predation, for both experiments. The probe-mark experiment
detected the removal of large infaunal polychaetes (∼ 20 mm), while the exclosure experiment showed depletion of epifaunal
harpacticoid copepods (0.063–0.5 mm). Predation on macrofaunal cumaceans was detected in both experiments. Invertebrates selected
by western sandpipers neither fell within traditional infaunal size classifications (macro- vs. meiofauna; 500 μm delineation)
nor corresponded to the highest densities of taxa. Rather, inference from experimental results and observations is that western
sandpipers forage in two modes, by: (1) surface gleaning of epibenthic copepods and cumaceans in the macro- and meiofaunal
size ranges and (2) selective probing for larger infauna, such as polychaetes. These findings were facilitated by the combination
of methodologies employed.
Received: 29 December 1999 / Accepted: 11 September 2000 相似文献
999.
Biomass partitioning of benthic microbes in a Baltic inlet: relationships between bacteria, algae, heterotrophic flagellates and ciliates 总被引:6,自引:0,他引:6
The structure of a benthic microbial food web and its seasonal changes were studied in the shallow brackish waters of the
island of Hiddensee, northeastern Germany, at two sites in close proximity by monthly or bimonthly sampling from July 1995
to June 1996. Abundance and biomass of phototrophic and non-phototrophic bacteria, heterotrophic flagellates (HF) and ciliates
as well as the biomass of microphytobenthos were determined in the upper 0.3 cm sediment layer. Abundance of organisms showed
strong positive correlation with water temperature, with the exception of the bacteria. Non-phototrophic bacterial numbers
ranged from 7 × 108 to 6.7 × 109 cells cm−3 and phototrophic bacterial abundance from 4 × 107 to 2.7 × 108. Heterotrophic protist abundance ranged from 8 × 103 to 104 × 103 ind cm−3 for HF and from 39 to 747 ind cm−3 for ciliates. The biomass partitioning demonstrated the primary importance of non-phototrophic bacteria (min. 0.83, max.
84.87 μg C cm−3), followed by the microphytobenthos (min. 1.32, max. 50.93 μg C cm−3). The heterotrophic protists contributed roughly the same fraction to the total microbial biomass, with the biomass of the
HF being slightly higher (HF 0.23 to 1.76 μg C cm−3, ciliates 0.04 to 1.17 μg C cm−3). Taxonomic classification of the benthic HF revealed the euglenids to be the most important group in terms of abundance
and biomass, followed by thaumatomastigids and kinetoplastids. Other important groups were apusomonads, cercomonads, pedinellids
and cryptomonads. The structure of the HF assemblage showed strong seasonal changes with euglenids being the most abundant
taxa in summer, while apusomonads and thaumatomastigids were predominant in winter. Similar to the pelagic microbial food
web, benthic picophototrophic bacteria were occasionally abundant, and the feeding modes of heterotrophic protists exhibited
a great variety (predominantly omnivores, bacterivores, herbivores or predators). Filter-feeding HF were of little importance.
Contrary to the pelagic environment, a top-down control on total benthic bacterial numbers by HF seemed unlikely at the studied
stations which were characterised by muddy sand.
Received: 6 January 1999 / Accepted: 21 October 1999 相似文献
1000.
This paper reviews the impact of the literature in depletable resources and energy economics over the period 1973–1998, particularly the initial period of publication of the Journal of Environmental Economics and Management, 1974–1998. A discussion of prominent policy issues in this arena is provided, along with an indication of what academic economics papers have contributed to that debate. This is followed by a citation analysis of contributions in the fields of energy and exhaustible resource economics. For each of these two fields, a list of the top papers in each five-year period from 1974 to 1998 is presented, along with a list of the top journals in each decade, based on average citations per article. The top ten cited articles in the fields in the Journal of Environmental Economics and Management are also presented. 相似文献