A nonparametric procedure for analyzing repeated measures of spatially correlated data

Many agricultural, biological, and environmental studies involve detecting temporal changes of a response variable, based on data observed at sampling sites in a spatial region and repeatedly over several time points. That is, data are repeated measures over time and are potentially correlated across space. The traditional repeated-measures analysis allows for time dependence but assumes that the observations at different sampling sites are mutually independent, which may not be suitable for field data that are correlated across space. In this paper, a nonparametric large-sample inference procedure is developed to assess the time effects while accounting for the spatial dependence using a block bootstrap. For illustration, the methodology is applied to describe the population changes of root-lesion nematodes over time in a production field in Wisconsin.     

The Surface Roughness of a Rubber Soling Material Determines the Coefficient of Friction on Water-Lubricated Surfaces

For more than a decade, evidence has been accumulating that points to the fact that the microscopic roughness of the footwear soling surface is a major determinant of slip-resistance on lubricated surfaces, but conclusive experimental proof has been lacking. This article describes an experiment in which five pairs of shoes were soled with the same rubber compound. Four of the pairs were abraded by different grades of grit to produce a range of roughness values. The coefficient of friction (CoF) of the five solings was then measured repeatedly by the walking traction method on wet surfaces including glazed wall tiles, vinyl asbestos coated with the wax floor polish, and both sides of a sheet of float glass. The Kruskal-Wallis statistical test proved beyond doubt that the soling roughness is a major factor in determining the CoF of this rubber soling material; p < 10⁻⁵. Nearly all of the grip was due to surface roughness of the soling material on these atypically smooth floors, although the surface roughness of the floors also had a significant effect on CoF; p < 0.003. Float glass is shown to be a promising reference floor material for the measurement of CoF of footwear; there was no statistical difference between results for the two sides of the glass sheet. Float glass could be used in the development of a standard CoF test method because new sheets of glass from the same manufacturer are identical and extremely smooth. The specification of CoF values for solings/floors combinations in lubricated conditions is of little value unless associated with roughness measurements and knowledge of how wear will affect the surface roughness of the sole. This article reports the first evidence that any specification of flooring by measuring CoF based on dry surfaces could lead to an increase in the number of injuries caused by slipping on the wet surfaces. Published by Elsevier Science Ltd     

Bioaccumulation of PCBs in Arctic seabirds: influence of dietary exposure and congener biotransformation

Four seabird species and their prey (zooplankton or fish) were collected in the Barents Sea to determine how dietary exposure, cytochrome P450 (CYP) enzyme activities and sex influenced their hepatic PCB concentrations and accumulation patterns. Five males and five females from each seabird species (little auk (Alle alle), Brunnich's guillemot (Uria lomvia), black guillemot (Cepphus grylle) and black-legged kittiwake (Rissa tridactyla)) were analysed. PCB concentrations could not be explained directly by carbon source (delta13C) or trophic position (delta15N), but by a combination of dietary parameters (delta13C, delta15N, migratory pattern, age) and contaminant metabolism. Contrary to previous studies, the PCB pattern differed among seabirds, with a higher proportion of persistent congeners (% of PCB-153, RPCB-153) in black-legged kittiwake than in auks. The PCB pattern also differed among auks, with little auk as the most efficient biotransformer (highest RPCB-153 values of persistent congeners). Based on high RPCB-153 values, Brunnich's guillemot poorly metabolised ortho-meta-unsubstituted congeners, whereas black guillemot poorly metabolised meta-para unsubstituted congeners. Species-specific differences in PCB biotransformation were confirmed by metabolic indices, where PCB patterns in seabirds were adjusted for PCB pattern in prey. The relative contribution of ortho-meta-unsubstituted congeners to SigmaPCBs decreased with increasing EROD activity. There were no differences in PCB concentrations, PCB patterns or cytochrome P450 enzyme activities between males and females. CYP P450 activities (CYP1A- and CYP2B/3A-like: EROD and testosterone 6beta-hydroxylation, respectively) were low and did not correlate with concentrations of non- or mono-ortho Cl-substituted PCBs (NO- and MO-PCBs), or with total toxic equivalent concentrations (TEQs) for dioxin-like effects of NO- and MO-PCBs.     

Earthworms as biological monitors of cadmium, copper, lead and zinc in metalliferous soils

Earthworms (Lumbricus rebellus and Dendrodrilus rubidus) were sampled from one uncontaminated and fifteen metal-contaminated sites. Significant positive correlations were found between the earthworm and 'total' (conc. nitric acid-extractable) soil Cd, Cu, Pb and Zn concentrations (data log1) transformed). The relationships were linear, and the accumulation patterns for both species were similar when a single metal was considered, even though there were species difference in mean metal concentrations. Generally, the earthworm Cd concentration exceeded that of the soil; by contrast, the worm Pb concentration was lower than the soil Pb concentration in all but one (acidic, low soil Ca) site. Our observations suggest that Cu and Zn accumulation may be physiologically regulated by both species. Total-soil Cd explained 82-86% of the variability (V2) in earthworm Cd concentration; 52-58% of worm Pb and worm Zn concentrations were explained by the total-soil concentrations of the respective metals. Total-soil Cu explained only 11-32% of the worm Cu concentration. The effect of soil pH, total Ca concentration, cation-exchange capacity (CEC) and organic carbon on metal accumulation by L. rubellus and D. rubidus was investigated by multiple regression analysis. Soil pH (coupled with CEC) and soil Ca had a major influence on Pb accumulation (V2 of worm Pb increased to 77-83%), and there was some evidence that Cd accumulation may be suppressed in extremely organic soils. The edaphic factors investigated had no effect on Cu or Zn accumulation by earthworms. In the context of biomonitoring, it is proposed that earthworms have a potential in a dual role: (1) as 'quantitative' monitors of total-soil metal concentrations (as shown for Cd); and (2) as estimators of 'ecologically significant' soil metal, integrating the effects of edaphic factors (as shown for Pb).     

Are leaves that fall from imidacloprid-treated maple trees to control Asian longhorned beetles toxic to non-target decomposer organisms?

The systemic insecticide imidacloprid may be applied to deciduous trees for control of the Asian longhorned beetle, an invasive wood-boring insect. Senescent leaves falling from systemically treated trees contain imidacloprid concentrations that could pose a risk to natural decomposer organisms. We examined the effects of foliar imidacloprid concentrations on decomposer organisms by adding leaves from imidacloprid-treated sugar maple trees to aquatic and terrestrial microcosms under controlled laboratory conditions. Imidacloprid in maple leaves at realistic field concentrations (3-11 mg kg(-1)) did not affect survival of aquatic leaf-shredding insects or litter-dwelling earthworms. However, adverse sublethal effects at these concentrations were detected. Feeding rates by aquatic insects and earthworms were reduced, leaf decomposition (mass loss) was decreased, measurable weight losses occurred among earthworms, and aquatic and terrestrial microbial decomposition activity was significantly inhibited. Results of this study suggest that sugar maple trees systemically treated with imidacloprid to control Asian longhorned beetles may yield senescent leaves with residue levels sufficient to reduce natural decomposition processes in aquatic and terrestrial environments through adverse effects on non-target decomposer organisms.     

Response of Organic and Inorganic Carbon and Nitrogen to Long-Term Grazing of the Shortgrass Steppe

We investigated the influence of long-term (56 years) grazing on organic and inorganic carbon (C) and nitrogen (N) contents of the plant–soil system (to 90 cm depth) in shortgrass steppe of northeastern Colorado. Grazing treatments included continuous season-long (May–October) grazing by yearling heifers at heavy (60–75% utilization) and light (20–35% utilization) stocking rates, and nongrazed exclosures. The heavy stocking rate resulted in a plant community that was dominated (75% of biomass production) by the C₄ grass blue grama (Bouteloua gracilis), whereas excluding livestock grazing increased the production of C₃ grasses and prickly pear cactus (Opuntia polycantha). Soil organic C (SOC) and organic N were not significantly different between the light grazing and nongrazed treatments, whereas the heavy grazing treatment was 7.5 Mg ha^–1 higher in SOC than the nongrazed treatment. Lower ratios of net mineralized N to total organic N in both grazed compared to nongrazed treatments suggest that long-term grazing decreased the readily mineralizable fraction of soil organic matter. Heavy grazing affected soil inorganic C (SIC) more than the SOC. The heavy grazing treatment was 23.8 Mg ha^–1 higher in total soil C (0–90 cm) than the nongrazed treatment, with 68% (16.3 Mg ha^–1) attributable to higher SIC, and 32% (7.5 Mg ha^–1) to higher SOC. These results emphasize the importance in semiarid and arid ecosystems of including inorganic C in assessments of the mass and distribution of plant–soil C and in evaluations of the impacts of grazing management on C sequestration.     

Estimates of Particle Hygroscopicity during the Southeastern Aerosol and Visibility Study

ABSTRACT

Aerosol water content was determined from relative humidity controlled optical particle counter (ASASP-X) size distribution measurements made during the Southeastern Aerosol and Visibility Study (SEAVS) in the Great Smoky Mountains National Park during summer 1995. Since the scattering response function of the ASASP-X is sensitive to particle refractive index, a technique for calibrating the ASASP-X for any real refractive index was developed. A new iterative process was employed to calculate water mass concentration and wet refractive index as functions of relative humidity. Experimental water mass concentrations were compared to theoretically predicted values assuming only ammonium sulfate compounds were hygroscopic. These comparisons agreed within experimental uncertainty. Estimates of particle hygroscopicity using a rural aerosol model of refractive index as a function of relative humidity demonstrated no significant differences from those made with daily varying refractive index estimates. Although aerosol size parameters were affected by the assumed chemical composition, forming ratios of these parameters nearly canceled these effects.     

Response to “Comment on ‘Use of local tidal records to identify relative sea level change: accuracy and error for decision makers’ by Powell VA, McGlashan DJ, Duck RW (2012) J Coast Conserv” by Rickards L, Brown J, Hibbert A, Woodworth P, Plater AJ (2013) J Coast Conserv

Tide gauge data have become increasing accessible to non-specialists through the availability of records over the Internet. Our original paper, “Use of local tidal records to identify relative sea level change: accuracy and error for decision makers”, focused on highlighting the potential pitfalls in tide gauge data manipulation that non-specialists may not be aware of. Since publication, Rickards et al. have made helpful observations and raised queries about certain elements within our paper, which we respond to here.     

Use of local tidal records to identify relative sea level change: accuracy and error for decision makers

The Intergovernmental Panel on Climate Change (IPCC) considers eustatic sea level rise to be a major impact driven by climate change. Relative sea level change, whether positive or negative, will affect industries, communities and ecology along the world??s coastlines and estuaries. Estimates of global eustatic sea level rise between 1961 and 2003 are 1.8?±?0.5?mm a^?1, reflecting results from validated global tide gauge records. Over the last two decades, several studies have used automatic tide gauge records with at least 80?years of data to generate global prediction models. The IPCC recognises that global change is not uniform, therefore local policy for flood management and coastal protection should rely on local change models that incorporate glacio-isostatic adjustment (GIA) and apply accurate data correction techniques. Some of the longest tidal records are held within the Northern Hemisphere, e.g. Cascais, Amsterdam, Aberdeen, Sheerness and Newlyn. The UK provides several important case studies highlighting changes in relative sea level between the north and the south, primarily due to variations in GIA rates of land uplift and subsidence. Tide gauge records are held by a variety of governmental, non-governmental and private organisations. However, each source may typically compile data in different ways, relying on diverse equipment and recording techniques, often with variations in frequency, length, quality and corrections applied. Even within a single organisation there may be differences in dataset quality. This paper examines some of the key sources of error when working with historical tidal datasets in local geographic areas and aims to identify the limitations of locally derived data thereby assisting in the determination of relative sea level trends that are of widespread value to infrastructure and policy makers.