Interrelationships of the two families constituting the Lophogastrida (Crustacea: Mysidacea) inferred from morphological and molecular data

The Lophogastrida are primitive Mysidacea and comprise only six genera. One of these, Eucopia is considered as highly specialized and constitutes the family Eucopiidae; the other genera constitute the Lophogastridae. Among the latter family, the genus Gnathophausia is closely related to Eucopia, with two species (G. gracilis and E. sculpticauda) sharing similar morphological characteristics [i.e. ornamentation (spines) of the uropods, and the gastric mill]. This indicates that these species are phylogenetically related. To test this hypothesis, the partial 16S mitochondrial ribosomal RNA gene from various representative species of Gnathophausia and Eucopia were compared. The resulting phylogenetic tree suggests that each genus is monophyletic, and that Gnathophausia, which is the deepest-branching genus, is the most primitive, with the Eucopiidae originating from the Lophogastridae. The molecular results support the morphological hypothesis, and suggest an early separation of the two genera or a rapid divergence of Eucopia due to morphological specialization. Received: 29 June 1997 / Accepted: 16 March 1998     

Effects of ozone fumigation treatment on the removal of residual difenoconazole from strawberries and on their quality

The effect of ozone fumigation on the reduction of difenoconazole residue on strawberries was studied. Strawberries were immersed in 1.0 L of aqueous solution containing 400 μL of the commercial product (250 g L^?1 of difenoconazole) for 1 min. Then, they were dried and exposed to ozone gas (O₃) at concentrations of 0.3, 0.6 and 0.8 mg L^?1 for 1 h. The ozone fumigation treatments reduced the difenoconazole residue on strawberries to concentrations below 0.5 mg kg^?1, which corresponds to a 95% reduction. The strawberries treated with ozone and the control group, which was not treated with ozone, were stored at 4°C for 10 days. Some characteristics of the fruit were monitored throughout this period. Among these, pH, weight loss and total color difference did not change significantly (P > 0.05). The fumigation with ozone significantly affected the soluble solids, titratable acidity and ascorbic acid content (vitamin C) of the strawberries preventing a sharp reduction of these parameters during storage.     

Influence of sugar cane vinasse on the sorption and degradation of herbicides in soil under controlled conditions

This study reports the influence of sugar cane vinasse on the persistence, sorption and leaching potential of diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea), hexazinone (3-cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4-dione) and tebuthiuron (1-(5-tert-butyl-1,3,4-thiadiazol-2-yl)-1,3-dimethylurea) in both a clay and sandy soil from a tropical area of Brazil. The experiments were conducted out under controlled laboratory conditions. The addition of sugarcane vinasse to soil influenced the persistence and sorption of the herbicides in both the studied clay and sandy soils, with a considerable decrease in the diuron DT₅₀ values in clay soil. The Ground Water Ubiquity Score (GUS) Index classifies the herbicides as leachers in both soils and treatments, with the exception of diuron, which is classified as a non-leacher in clay soil-vinasse and as a transient herbicide in sandy soil. These results suggest that special attention should be given to areas such as those where the sandy soil was collected in this study, which is a recharge area of the Guarani Aquifer and is likely to experience groundwater contamination due to the high leaching potential of the applied pesticides.     

Long-term sustainability of metal immobilization by soil amendments: Cyclonic ashes versus lime addition

A soil column leaching experiment was used to gain insight into the long-term metal immobilization capacity of cyclonic ashes (CAH) compared to lime (LIME). Twenty six years of rainfall were simulated. Initially, all amended soils were brought to an equal soil pH. This was done to obtain optimal conditions for the detection of metal immobilization mechanisms different from just a pH effect. During the simulation period, soil pH in all treatments decreased in parallel. However, the evolution of metal mobility and phytoavailability showed a clearly distinct pattern. The strong reduction in metal immobilizing efficiency observed in the lime treatment at the end of the simulation period was much less pronounced, or even absent, in the CAH treatments. Moreover, metal accumulation in plants grown on the CAH amended soil was significantly lower compared to the untreated and the lime treated soil. CAH + SS treatment delivered the strongest reductions in metal mobility and bioavailability.     

Regulatory structures and operational environment in the Portuguese waste sector

This research computes the influence of the operational environment on the efficiency of the Portuguese urban solid waste services. A sample of 29 solid waste utilities encompassing the whole continental country was used for this purpose. Particularly, we apply the non-parametric double bootstrap model to estimate the effect of various explanatory factors on robust data envelopment analysis estimates. In general, we find a significant influence of the environmental context on the solid waste utilities’ performance. The environmental context is characterized by gross domestic product per capita, distance to treatment facilities, population density, regulation, type of management, composting and incineration services.     

Dissipation and translocation of saisenxin in tobacco and soil under conventional field and controlled laboratory conditions

Abstract

The aim of this investigation was to investigate the fate and translocation characteristics of saisenxin (SSX), a novel organic zinc fungicide, in the environment and tobacco plants under conventional field and laboratory conditions. A rapid and sensitive analytical technique based on high-performance liquid chromatography was used for determination of SSX, in soil samples and tobacco leaf, stem and root samples. The method had satisfactiry linearity (R^2?=?0.9999) and the limits of detection and of quantitation of the target compound were 0.06 and 0.20?mg kg^?1, respectively. The average recoveries were in the range of 89.74–94.24% in soil, leaf, stem and root samples, with relative standard deviations of <8%. For conventional field trials, the half-life (t_1/2) of SSX was 5.9–6.5 days in soil and 4.8–5.3 days in tobacco leaves; the corresponding values under controlled laboratory conditions were extended to 7.1 and 7.6?days. The translocation factor (TF) values were in the range of 0–2.25 and 0–0.25 for foliage and root irrigation treatments, respectively. The TFs of SSX in tobacco indicated that tobacco had a high ability to transfer SSX upward.     

The Relationship between “Soiling Index” and Suspended Particulate Matter Concentrations

Experimental relationships between the mass concentration of suspended particulate matter and the optical density of particulates collected on paper tape have been determined for the atmospheric aerosol and for aerosols of constant optical properties. Simultaneous samples were obtained on membrane filters (for gravimetric analysis) and on Whatman No. 4 paper tape (for optical evaluation). Sampling procedures were adopted which ensured that the efficiency of sampling was the same in both cases.

Consistent relationships between mass concentrations of suspended particulate matter and optical density expressed in terms of per cent transmittance or per cent reflectance were found for dispersions of coal, limestone, fly ash, and a coal-limestone mixture.

For atmospheric aerosol samples collected on the roof of the Graduate School of Public Health, University of Pittsburgh, the relationship between mass concentration and per cent transmittance or per cent reflectance was found to be linear over the range of values observed. The correlation coefficients were ?0.93 (for concentration versus per cent transmittance) and ?0.89 (for concentration versus per cent reflectance).     

Using forest historical information to target landscape ecological restoration in Southwestern Patagonia

Ambio - Ecotonal zones between eastern semi-arid steppes and Nothofagus spp. forests in western Patagonia are the result of broad ecosystem changes, which have intensified in the last...     

Aluminium: The Need for a Re-Evaluation of Its Toxicity and Solubility in Mature Forest Stands

Aluminium (Al) is a key element in critical loadcalculations for forest. Here, we argue for re-evaluating theimportance of Al. Effects of two levels of enhanced Alconcentrations and lowered Ca:Al ratios in the soil solutionin a field manipulation experiment in a mature spruce stand(1996–1999) on tree vitality parameters were tested. Inaddition, Al solubility controls were tested. Various loads ofAl were added to forest plots by means of an irrigationsystem. Potentially toxic Al concentrations and criticalratios of Ca to inorganic Al were established. The ratio of Cato total Al was not a suitable indicator for unfavourableconditions for plant growth. No significant effects on crowncondition, tree growth and fine root production were observedafter three years of treatment. In 1999, foliar Mg content inthe highest Al addition treatment had declined significantly.This agreed with the known response to Al stress of seedlingsin nutrient solution experiments. No support was found forusing the chemical criterion Ca:Al ratio in soil solution,foliar and root tissue as an indicator for forest damage dueto acidification. Al solubility was considerably lower thanimplied by the assumption of equilibrium with gibbsite,particularly in the root zone. The gibbsite equilibrium iscommonly used in critical load models. Substitution of thegibbsite equilibrium with an Al-organic matter complexationmodel to describe Al solubility in soil water may have largeconsequences for calculation of critical loads. The resultsindicate that critical load maps for forests should bereconsidered.