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Sofia A. Kyriakeas Mary C. Watzin 《Journal of the American Water Resources Association》2006,42(2):425-441
Benthic macroinvertebrate communities in streams adjacent to cornfields, streams where cows had unrestricted access, and reference locations without agriculture were compared to examine the effects of local land use and land use/land cover in the watershed. At each local site, macroinvertebrates and a variety of habitat parameters were measured upstream, adjacent, downstream, and farther downstream of the local land use. A geographic information system (GIS) was used to calculate drainage basin area, land use/land cover percentages in each basin, and the distance from sample sites to the stream source. Three‐way analysis of covariance (ANCOVA) tests with date, site type, and sampling location as main effects were used to explore differences in macroinvertebrate metrics using median substrate size, percent hay/pasture area, and stream depth as covariates. The covariates significantly improved model fit and showed that multiple contributing factors influence community composition. Local impacts were greatest at sites where cows had access, probably because of sedimentation and embeddedness in the substrate. Differences between the upstream and the adjacent and downstream locations were not as great as expected, perhaps because upstream recolonization was reduced by agricultural impacts or because of differences in the intensity or proximity of agriculture to riparian areas in the watershed. The results underscore the importance of both local and watershed factors in controlling stream community composition. 相似文献
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Alan R. Thornhill John A. McGrath Robin A. J. Eady Peter R. Braude Alan H. Handyside 《黑龙江环境通报》2001,21(6):490-497
Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR-based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. A number of different strategies (including the use of lysis buffers to break down the cell and make the DNA accessible) have been employed to combat ADO with varying degrees of success, yet there is still no consensus among PGD centres over which lysis buffer should be used (ESHRE PGD Consortium, 1999 ). To address this issue, PCR amplification of three genes (CFTR, LAMA3 and PKP1) at different chromosomal loci was investigated. Single lymphocytes from individuals heterozygous for mutations within each of the three genes were collected and lysed in either alkaline lysis buffer (ALB) or proteinase K/SDS lysis buffer (PK). PCR amplification efficiencies were comparable between alkaline lysis and proteinase K lysis for PCR products spanning each of the three mutated loci (ΔF508 in CFTR 90% vs 88%; R650X in LAMA3 82% vs 78%; and Y71X in PKP1 91% vs 87%). While there was no appreciable difference between ADO rates between the two lysis buffers for the LAMA3 PCR product (25% vs 26%), there were significant differences in ADO rates between ALB and PK for the CFTR PCR product (0% vs 23%) and the PKP1 PCR product (8% vs 56%). Based on these results, we are currently using ALB in preference to PK/SDS buffer for the lysis of cells in clinical PGD. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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Steinar Sandφy 《Ambio-人类环境杂志》2003,32(3):162-164
20世纪70和80年代,"酸雨"成为一个主要公共政策问题.在加拿大和北欧的斯堪的纳维亚半岛的酸化水体中,生物群落及生境遭到频繁破坏,诸如土著鲑鱼和鳟鱼种群受损乃至灭绝的现象屡见不鲜. 相似文献
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Whereas past research has treated co-management of common pool resources as if villagers and project implementing authorities
were the only relevant actors, numerous external factors beyond the control of these two partners create barriers to successful
co-management. This paper draws on discussions with Forest Department officials to examine the influence of these forces on
the outcomes of Joint Forest Management (JFM) in Tamil Nadu, India. An empirical inquiry into the operational aspects of JFM
indicates the important roles of political parties, powerful people, and other state institutions and functionaries as well
as the flow of foreign funding. Further, the strong demand by local people for socio-economic development interventions as
opposed to improvement of degraded forests belittles the role of the Forest Department relative to other departments. Numerous
other conditioning factors and relationships are explored. The authors call for reforms in public governance to allow better
participation of all the actors involved for this participatory management approach to succeed and sustain. 相似文献
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John F. Potter 《The Environmentalist》2006,26(4):329-329
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