Identification and quantitation of semi-crystalline microplastics using image analysis and differential scanning calorimetry

Environmental Science and Pollution Research - There are several techniques used to analyze microplastics. These are often based on a combination of visual and spectroscopic techniques. Here we...     

Perceptions regarding the challenges and constraints faced by smallholder farmers of vanilla in Mexico

Mexico was the main vanilla producer worldwide many years ago. However, it currently provides just around 5 % of the global production. The issues that have led to the current stagnation of the vanilla production in Mexico have been addressed from various perspectives, but few studies have included the opinion of smallholder farmers. For this reason, the aim of this study was to conduct a participatory diagnostic to identify the challenges and constraints that affect the vanilla productive sector in Mexico from the smallholder farmers perspective. This study was conducted under the of Participatory Rural Appraisal (PRA) approach; consequently, we used PRA techniques for data collection. The qualitative data obtained were transcribed and analyzed using the Content Analysis Method (CAM) and Constant Comparative Method (CCM). The results showed that most of the challenges and limitations referred to by smallholder farmers result from the lack of training to improve production, processing, and marketing. Likewise, various restrictive aspects were identified in the production process that affect crop productivity, such as cultivation in small parcels, high incidence of diseases, premature abortion of fruits, and low tolerance of plants to stress. An aspect common to all processes is the need to promote organization and training schemes, since under the conditions described for the vanilla production chain in Mexico, relationships have been built in highly competitive communities and, in general, the targets seek have been mutually exclusive. For the above, we consider that achieving more profitable and sustainable production schemes require placing smallholder farmers at the base of an inclusive production system supported by fair trade, with organization, institutions, technology transfer, level of trust, and cooperation as core elements.     

The optimal pricing of pollution when enforcement is costly

We consider the pricing of a uniformly mixed pollutant with a model of optimal, possibly firm-specific, emissions taxes and their enforcement under incomplete information about firms’ abatement costs, enforcement costs, and pollution damage. We argue that optimality requires an enforcement strategy that induces full compliance by every firm, except possibly when a regulator can base the probabilities of detecting individual violations on observable correlates of violators’ actual emissions. Moreover, optimality requires discriminatory taxes, except when a regulator is unable to use observable firm-level characteristics to gain some information about the variation in firms’ abatement costs or monitoring costs.     

Retention and rainfastness of mancozeb as affected by physicochemical characteristics of adaxial apple leaf surface after enhanced UV-B radiation

It is not clear so far whether alteration of leaf micromorphology and surface wax chemistry due to the impact of environmental factors, such as UV-B radiation, affects retention and rainfastness of applied pesticide solutions. In this study; UV-B treated and untreated adaxial leaf surfaces of apple seedlings (Malus domestica Borkh.) were characterized in terms of chemical composition, micromorphological fine structure, hydrophobicity, and wettability. Furthermore, the retention and rainfastness of applied fungicide mancozeb were studied. The samples were examined 0, 24 and 48 h after ultraviolet (UV)-B radiation (0.022 kW m^{? 2} for 150 min) The total wax mass, recovered from the adaxial leaf surface, amounted from 0.38 μ g cm^{? 2} (control) up to 0.49 μ g cm^{? 2} (24 h). Chemical composition of surface wax altered, whereas the contact angle of applied water droplets on leaf surface of UV-B treated plants did not change significantly compared to the control. The alteration of surface wax quantity and quality significantly affected retention of a.i.; it increased at a sampling time of 24 h after UV-B irradiation, whereas rainfastness of the fungicide spray solution was not significantly influenced.     

Retention and rainfastness of mancozeb as affected by physicochemical characteristics of adaxial apple leaf surface after enhanced UV-B radiation

It is not clear so far whether alteration of leaf micromorphology and surface wax chemistry due to the impact of environmental factors, such as UV-B radiation, affects retention and rainfastness of applied pesticide solutions. In this study; UV-B treated and untreated adaxial leaf surfaces of apple seedlings (Malus domestica Borkh.) were characterized in terms of chemical composition, micromorphological fine structure, hydrophobicity, and wettability. Furthermore, the retention and rainfastness of applied fungicide mancozeb were studied. The samples were examined 0, 24 and 48 h after ultraviolet (UV)-B radiation (0.022 kW m(-2) for 150 min) The total wax mass, recovered from the adaxial leaf surface, amounted from 0.38 microg cm(-2) (control) up to 0.49 microg cm(-2) (24 h). Chemical composition of surface wax altered, whereas the contact angle of applied water droplets on leaf surface of UV-B treated plants did not change significantly compared to the control. The alteration of surface wax quantity and quality significantly affected retention of a.i.; it increased at a sampling time of 24 h after UV-B irradiation, whereas rainfastness of the fungicide spray solution was not significantly influenced.     

Mercury contamination in alligators (Melanosuchus niger) from Mamirauá Reservoir (Brazilian Amazon) and human health risk assessment

Mercury (Hg) concentrations in muscles of wild alligators (Melanosuchus niger) from the Mamirauá Reservoir (a reference area in the Brazilian Amazon) and the human health risks associated with its consumption were assessed. The mean Hg concentration in alligator muscles was 0.407?±?0.114 μg/g (N?=?61). Close to 5 % of the muscle samples showed Hg levels above the World Health Organization guideline for fish consumption (0.5 μg/g). A positive and significant relationship was observed between Hg concentrations in muscle and the age of the specimens. The dose-response approach suggests that close to 27.4 years is required for half of the exposed specimens to attain 0.5 μg/g. The hazard quotient (HQ) is a risk indicator which defines the ratio of exposure level and a toxicological reference dose. HQ resulted above the unity for all the specimens when the ingestion rate for riverine communities (200 g of muscle per day) is considered, indicating the existence of hazard. When the ingestion rate for market consumers (28.57 g/day) is considered, the risks are much lower (mean HQ?=?0.55), suggesting that such group is not at risk. The establishment of local and regional ingestion rates for riverine populations and market consumers is extremely recommended.     

Spatial and temporal dynamic of trophic relevant parameters in a subtropical coastal lagoon in Brazil

Coastal lagoons are ecologically and economically important environments but a relative low number of studies were carried out in subtropical and permanently closed coastal lagoons. The present study aimed at assessing the temporal and spatial dynamic of trophic relevant water quality parameters in the small, deep and freshwater Peri coastal lagoon, South Brazil. During the 19 sampled months (March/2008-September/2009) spatial homogeneity (horizontal and vertical) was registered in all seasons for all variables, a condition related to the strong wind influence and low human occupation in the lagoon watershed. Seasonal variations of the water quality could be observed and they can be explained mainly by variation on temperature, wind forces and direction and rainfall, characteristic from the subtropical weather. Comparing this study with two others conducted in Peri lagoon in 1996 and 1998, no critical differences that evidence alteration in the water quality were found, but climate differences may have influenced in some small variations observed. The use of four trophic state indices indicated that indices designed for temperate lakes are inappropriate for the subtropical Peri lagoon. The lagoon was classified as oligotrophic for nutrients concentrations and meso-eutrophic for transparency and chlorophyll-a, which can be explained by the high densities and monodominance of the cyanobacterium Cylindrospermopsis raciborskii and the high recycling rates observed in warmer water bodies, when compared to the temperate ones.     

Evidence of at least two evolutionary lineages in Melipona subnitida (Apidae, Meliponini) suggested by mtDNA variability and geometric morphometrics of forewings

Melipona subnitida, a tropical stingless bee, is an endemic species of the Brazilian northeast and exhibits great potential for honey and pollen production in addition to its role as one of the main pollinators of the Caatinga biome. To understand the genetic structure and better assist in the conservation of this species, we characterized the population variability of M. subnitida using geometric morphometrics of the forewing and cytochrome c oxidase I gene fragment sequencing. We collected workers from six localities in the northernmost distribution. Both methodologies indicated that the variability among the sampled populations is related both to the environment in which samples were collected and the geographical distance between the sampling sites, indicating that differentiation among the populations is due to the existence of at least evolutionary lineages. Molecular clock data suggest that this differentiation may have begun in the middle Pleistocene, approximately 396 kya. The conservation of all evolutionary lineages is important since they can present differential resistance to environmental changes, as resistance to drought and diseases.     

The impact of air pollution on COVID-19 pandemic varied within different cities in South America using different models

Environmental Science and Pollution Research - There is a rising concern that air pollution plays an important role in the COVID-19 pandemic. However, the results were not consistent on the...     

Trophic interactions of the endangered Southern river otter (Lontra provocax) in a Chilean Ramsar wetland inferred from prey sampling, fecal analysis, and stable isotopes

Non-invasive methodological approaches are highly recommended and commonly used to study the feeding ecology of elusive and threatened mammals. In this study, we use multiple lines of evidence to assess the feeding strategies of the endangered Southern river otter, by determining seasonal prey availability (electrofishing), analysis of undigested prey remains (spraints), and the use of stable isotopes (δ¹⁵N and δ¹³C) in otter spraints (n?=?262) and prey in a wetland ecosystem of southern Chile (39°49′S, 73°15′W). Fecal and isotopic analyses suggest that the otter diet is restricted to a few prey items, particularly the less-mobile, bottom-living, and larger prey such as crayfish (Samastacus spinifrons, 86.11 %) and crabs (Aegla spp., 32.45 %), supplemented opportunistically by cyprinids (Cyprinus carpio, 9.55 %) and catfish (Diplomystes camposensis, 5.66 %). The results suggest that the river otter is highly specialized in bottom foraging. Isotopic signatures of food sources and feces revealed a mid-upper trophic position for the Southern river otter, with either higher or lower δ¹⁵N values than their potential prey items. δ¹³C values for river otters were less enriched than their potential food resources. We suggest that due to their narrow trophic niche and possible dependence on only a few food items, this species may be highly vulnerable to the reduction in its prey populations. Finally, maintaining the ecological interactions between Southern river otters and their prey is considered a central priority for the survival of this endangered carnivore mammal.