Evaluation of SARS-CoV-2 in Indoor Air of Sina and Shahid Beheshti Hospitals and Patients' Houses

Food and Environmental Virology - Side by side air sampling was conducted using a PTFE filter membrane as dry sampler and an impinger containing a suitable culture medium as a wet sampler. Most of...     

Active Surveillance and Genetic Characterization of Prevalent Velogenic Newcastle Disease and Highly Pathogenic Avian Influenza H5N8 Viruses Among Migratory Wild Birds in Southern Egypt During 2015–2018

Food and Environmental Virology - A total of 1007 samples (910 fecal droplets and 97 cloacal swabs) were collected from 14 species of migratory wild birds in most wetlands during 3 successive...     

Detection of Y chromosome-specific DNA in the plasma and urine of pregnant women using nested polymerase chain reaction

The present study was undertaken to evaluate a nested polymerase chain reaction (PCR) for detection of Y chromosome-specific fetal DNA in maternal plasma and urine of pregnant women during different gestational stages. DNA isolated from plasma and urine samples of 80 pregnant women (between 7 and 40 weeks' gestation) underwent amplification for Y chromosome-specific 198 bp DNA by nested PCR. The postpartum analysis of fetal gender showed that 55 women carried male and 25 female fetuses. Among the 55 women bearing male fetuses, Y chromosome-specific signals were detected in 53 (96%) plasma and 21 (38%) urine samples. Moreover, out of 25 women bearing female fetuses, 3 (12%) and 1 (4%) women had Y chromosome-specific signal in plasma and urine, respectively. Analysis of results with respect to gestational age revealed that there was no significant difference in the detection of Y chromosome-specific DNA between different trimesters in maternal plasma of women bearing male fetuses. These results showed that fetus-specific DNA was detected with high sensitivity (96%) and specificity (88%) in the maternal plasma by nested PCR, and therefore the method could be useful as a non-invasive procedure for fetal sex determination and prenatal diagnosis. Copyright © 2001 John Wiley & Sons, Ltd.     

Protective effect of Spirulina platensis against aluminium-induced nephrotoxicity and DNA damage in rats

The protective effect of Spirulina platensis (SP) powder against aluminium-induced nephrotoxicity and DNA damage in rats was studied. Male rats receiving daily 40 mg/kg b.wt. aluminium chloride (AlCl₃) orally had increased serum levels of urea and creatinine, up regulated kidney injury molecule-1 and tissue inhibitor of metalloproteinase-1 genes, down regulated catalase and glutathione peroxidase genes, and increased all parameters of kidney DNA damage using comet assay. Treatment with SP alleviated all AlCl₃-induced effects of toxicity, especially when the animals were pre-treated.     

Synthesis,extraction, and identification of light polycyclic aromatic hydrocarbons (PAH) in microwave oven fried Aseel chicken tissues

The present study was conducted to determine the effects of microwave oven frying on production of four, light polycyclic aromatic hydrocarbons (PAH) anthracene, fluorene, phenathrene, and pyrene in four tissues heart, liver, neck, and wings of Aseel chickens. The microwave oven-fried samples of tissues along with respective control after appropriate preparation were analyzed using high-performance liquid chromatography. These PAH were identified by comparing retention time of the unknown with known ones and concentration was calculated. Data showed that among the four chicken tissues, the neck contained the maximum level (0.079 mg/kg) of anthracene while wings contained the minimum (0.006 mg/kg). Similarly, in case of flourene, the fried heart showed greater percentage increase (225.04%) while wings showed 16.18% fluorene compared with other two organs, while phenanthrene and pyrene were maximal in wings at 0.249 mg/kg and 0.094 mg/kg, respectively. Results demonstrate that high-temperature treatment such as microwave oven frying of chicken meat might be responsible to generate these toxicants. Further study is to determine the production of other PAH under the different barbecuing conditions using various meat varieties.     

Effect of sediment properties on the sorption of C12-2-LAS in marine and estuarine sediments

Linear alkylbenzene sulfonates (LAS) are anionic high production volume surfactants used in the manufacture of cleaning products. Here, we have studied the effect of the characteristics of marine and estuarine sediments on the sorption of LAS. Sorption experiments were performed with single sediment materials (pure clays and sea sand), with sediments treated to reduce their organic carbon content, and with field marine and estuarine sediments. C₁₂-2-LAS was used as a model compound. Sorption to the clays montmorillonite and kaolinite resulted in non-linear isotherms very similar for both clays. When reducing the organic content, sorption coefficients decreased proportionally to the fraction removed in fine grain sediments but this was not the case for the sandy sediment. The correlation of the sediment characteristics with the sorption coefficients at different surfactant concentrations showed that at concentrations below 10 μg C₁₂-2-LAS/L, the clay content correlated better with sorption, while the organic fraction became more significant at higher concentrations.